LOCUS       sequence-252944-       11690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 11690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH70
FEATURES             Location/Qualifiers
     source          1..11690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2023..2049,2050..2052,2053..6126)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6139..6159
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6352..6417
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6424..7047
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             7051..7071
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7093..7345
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7393..7417
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(7573..8721)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(9057..9645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9137..9156)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9816..10607,10608..10676))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10439..10458
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10677..10781)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10868..11499
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    11591..11615
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtgcagc gtgacccggt cgtgcccctc
       61 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
      121 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
      181 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
      241 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
      301 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
      361 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
      421 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
      481 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
      541 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
      601 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
      661 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
      721 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
      781 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg
      841 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      901 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      961 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
     1021 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1081 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1141 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1201 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1261 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1321 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1381 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct
     1441 ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt
     1501 gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact
     1561 gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg
     1621 gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt
     1681 aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg
     1741 aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga
     1801 tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac
     1861 aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct
     1921 atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt
     1981 ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gaatggataa gaagtactct
     2041 atcggactcg ctatcggaac taactctgtg ggatgggctg tgatcaccga tgagtacaag
     2101 gtgccatcta agaagttcaa ggttctcgga aacaccgata ggcactctat caagaaaaac
     2161 cttatcggtg ctctcctctt cgattctggt gaaactgctg aggctaccag actcaagaga
     2221 accgctagaa gaaggtacac cagaagaaag aacaggatct gctacctcca agagatcttc
     2281 tctaacgaga tggctaaagt ggatgattca ttcttccaca ggctcgaaga gtcattcctc
     2341 gtggaagaag ataagaagca cgagaggcac cctatcttcg gaaacatcgt tgatgaggtg
     2401 gcataccacg agaagtaccc tactatctac cacctcagaa agaagctcgt tgattctact
     2461 gataaggctg atctcaggct catctacctc gctctcgctc acatgatcaa gttcagagga
     2521 cacttcctca tcgagggtga tctcaaccct gataactctg atgtggataa gttgttcatc
     2581 cagctcgtgc agacctacaa ccagcttttc gaagagaacc ctatcaacgc ttcaggtgtg
     2641 gatgctaagg ctatcctctc tgctaggctc tctaagtcaa gaaggcttga gaacctcatt
     2701 gctcagctcc ctggtgagaa gaagaacgga cttttcggaa acttgatcgc tctctctctc
     2761 ggactcaccc ctaacttcaa gtctaacttc gatctcgctg aggatgcaaa gctccagctc
     2821 tcaaaggata cctacgatga tgatctcgat aacctcctcg ctcagatcgg agatcagtac
     2881 gctgatttgt tcctcgctgc taagaacctc tctgatgcta tcctcctcag tgatatcctc
     2941 agagtgaaca ccgagatcac caaggctcca ctctcagctt ctatgatcaa gagatacgat
     3001 gagcaccacc aggatctcac acttctcaag gctcttgtta gacagcagct cccagagaag
     3061 tacaaagaga ttttcttcga tcagtctaag aacggatacg ctggttacat cgatggtggt
     3121 gcatctcaag aagagttcta caagttcatc aagcctatcc tcgagaagat ggatggaacc
     3181 gaggaactcc tcgtgaagct caatagagag gatcttctca gaaagcagag gaccttcgat
     3241 aacggatcta tccctcatca gatccacctc ggagagttgc acgctatcct tagaaggcaa
     3301 gaggatttct acccattcct caaggataac agggaaaaga ttgagaagat tctcaccttc
     3361 agaatccctt actacgtggg acctctcgct agaggaaact caagattcgc ttggatgacc
     3421 agaaagtctg aggaaaccat caccccttgg aacttcgaag aggtggtgga taagggtgct
     3481 agtgctcagt ctttcatcga gaggatgacc aacttcgata agaaccttcc aaacgagaag
     3541 gtgctcccta agcactcttt gctctacgag tacttcaccg tgtacaacga gttgaccaag
     3601 gttaagtacg tgaccgaggg aatgaggaag cctgcttttt tgtcaggtga gcaaaagaag
     3661 gctatcgttg atctcttgtt caagaccaac agaaaggtga ccgtgaagca gctcaaagag
     3721 gattacttca agaaaatcga gtgcttcgat tcagttgaga tttctggtgt tgaggatagg
     3781 ttcaacgcat ctctcggaac ctaccacgat ctcctcaaga tcattaagga taaggatttc
     3841 ttggataacg aggaaaacga ggatatcttg gaggatatcg ttcttaccct caccctcttt
     3901 gaagatagag agatgattga agaaaggctc aagacctacg ctcatctctt cgatgataag
     3961 gtgatgaagc agttgaagag aagaagatac actggttggg gaaggctctc aagaaagctc
     4021 attaacggaa tcagggataa gcagtctgga aagacaatcc ttgatttcct caagtctgat
     4081 ggattcgcta acagaaactt catgcagctc atccacgatg attctctcac ctttaaagag
     4141 gatatccaga aggctcaggt ttcaggacag ggtgatagtc tccatgagca tatcgctaac
     4201 ctcgctggat ctcctgcaat caagaaggga atcctccaga ctgtgaaggt tgtggatgag
     4261 ttggtgaagg tgatgggaag gcataagcct gagaacatcg tgatcgaaat ggctagagag
     4321 aaccagacca ctcagaaggg acagaagaac tctagggaaa ggatgaagag gatcgaggaa
     4381 ggtatcaaag agcttggatc tcagatcctc aaagagcacc ctgttgagaa cactcagctc
     4441 cagaatgaga agctctacct ctactacctc cagaacggaa gggatatgta tgtggatcaa
     4501 gagttggata tcaacaggct ctctgattac gatgttgatc atatcgtgcc acagtcattc
     4561 ttgaaggatg attctatcga taacaaggtg ctcaccaggt ctgataagaa caggggtaag
     4621 agtgataacg tgccaagtga agaggttgtg aagaaaatga agaactattg gaggcagctc
     4681 ctcaacgcta agctcatcac tcagagaaag ttcgataact tgactaaggc tgagagggga
     4741 ggactctctg aattggataa ggcaggattc atcaagaggc agcttgtgga aaccaggcag
     4801 atcactaagc acgttgcaca gatcctcgat tctaggatga acaccaagta cgatgagaac
     4861 gataagttga tcagggaagt gaaggttatc accctcaagt caaagctcgt gtctgatttc
     4921 agaaaggatt tccaattcta caaggtgagg gaaatcaaca actaccacca cgctcacgat
     4981 gcttacctta acgctgttgt tggaaccgct ctcatcaaga agtatcctaa gctcgagtca
     5041 gagttcgtgt acggtgatta caaggtgtac gatgtgagga agatgatcgc taagtctgag
     5101 caagagatcg gaaaggctac cgctaagtat ttcttctact ctaacatcat gaatttcttc
     5161 aagaccgaga ttaccctcgc taacggtgag atcagaaaga ggccactcat cgagacaaac
     5221 ggtgaaacag gtgagatcgt gtgggataag ggaagggatt tcgctaccgt tagaaaggtg
     5281 ctctctatgc cacaggtgaa catcgttaag aaaaccgagg tgcagaccgg tggattctct
     5341 aaagagtcta tcctccctaa gaggaactct gataagctca ttgctaggaa gaaggattgg
     5401 gaccctaaga aatacggtgg tttcgattct cctaccgtgg cttactctgt tctcgttgtg
     5461 gctaaggttg agaagggaaa gagtaagaag ctcaagtctg ttaaggaact tctcggaatc
     5521 actatcatgg aaaggtcatc tttcgagaag aacccaatcg atttcctcga ggctaaggga
     5581 tacaaagagg ttaagaagga tctcatcatc aagctcccaa agtactcact cttcgaactc
     5641 gagaacggta gaaagaggat gctcgcttct gctggtgagc ttcaaaaggg aaacgagctt
     5701 gctctcccat ctaagtacgt taactttctt tacctcgctt ctcactacga gaagttgaag
     5761 ggatctccag aagataacga gcagaagcaa cttttcgttg agcagcacaa gcactacttg
     5821 gatgagatca tcgagcagat ctctgagttc tctaaaaggg tgatcctcgc tgatgcaaac
     5881 ctcgataagg tgttgtctgc ttacaacaag cacagagata agcctatcag ggaacaggca
     5941 gagaacatca tccatctctt cacccttacc aacctcggtg ctcctgctgc tttcaagtac
     6001 ttcgatacaa ccatcgatag gaagagatac acctctacca aagaagtgct cgatgctacc
     6061 ctcatccatc agtctatcac tggactctac gagactagga tcgatctctc acagctcggt
     6121 ggtgattcaa gggctgatcc taagaagaag aggaaggttg gaggagggcc aggagcagag
     6181 tatgttagag cgttgtttga ttttaacggt aacgatgaag aggatttacc ctttaagaaa
     6241 ggcgacattc tcaggattag ggataaacct gaagagcaat ggtggaatgc tgaggatagt
     6301 gaaggcaaac gaggaatgat tttagtgccg tatgtggaga aatattcggg tgactacaaa
     6361 gatcatgatg gtgattacaa agaccatgac atcgactaca aggatgatga tgataagtca
     6421 gggatgacag atgctgaata tgtcagaatc cacgaaaagt tggacattta cacgtttaag
     6481 aagcagttct tcaacaacaa gaaatctgtt tcgcataggt gctatgtgct tttcgaacta
     6541 aaacgtcgtg gagaaagacg ggcttgcttt tggggttacg cggttaacaa accacaatca
     6601 ggtactgaac gaggaataca cgctgaaatc ttttctatcc gaaaggttga ggaatatcta
     6661 cgtgacaatc ctggacagtt cactatcaat tggtattcta gctggtcacc atgtgcagat
     6721 tgtgctgaga agattctcga atggtacaat caagagctta gaggcaatgg acatacattg
     6781 aaaatatggg catgcaagct ctactacgaa aagaatgcca gaaaccaaat tgggctttgg
     6841 aacttgaggg ataatggagt tgggcttaat gtcatggttt ctgagcacta tcaatgttgt
     6901 cggaagatct tcatacaaag ttcccataac cagttgaatg agaacagatg gttagagaaa
     6961 acccttaaaa gagccgagaa gagaagatcc gaactgagca ttatgataca ggtcaaaatt
     7021 ctgcatacca ctaagagtcc agctgtaggt cctaagaaga aacgtaaagt agggccctga
     7081 gcttgtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     7141 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     7201 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     7261 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     7321 gcggtgtcat ctatgttact agatcgacgc tactattgtc ttctgcacga agtggtttaa
     7381 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     7441 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     7501 atgccaacca cagggttccc cagatcaggc gctggctgct gaacccccag ccggaactga
     7561 ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac
     7621 caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac
     7681 gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc
     7741 ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt
     7801 ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat
     7861 caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag
     7921 cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag
     7981 gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc
     8041 ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta
     8101 ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta
     8161 ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg
     8221 gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg
     8281 catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg
     8341 cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc
     8401 gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt
     8461 cgccaaacct gccgcctcct gttcaagacg acgcgaacgc tccacggcgg ccgatggcgc
     8521 gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg
     8581 gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc
     8641 gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt
     8701 ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc
     8761 aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt
     8821 atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg
     8881 aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc
     8941 ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc
     9001 gttgcgccac atctaggatc tgccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9061 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9481 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9901 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagaa
     9961 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10021 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10081 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    10141 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10201 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10261 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10321 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10381 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10441 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10501 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10561 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10621 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10681 ttcctttttc aatattattg aagcatttat caaggttatt gtctcatgag cggatacata
    10741 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacgaattg gccagcgctg
    10801 ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag cccctggggg gatgggaggc
    10861 ccgcgttagc gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt
    10921 cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca
    10981 ggttaaaaga caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat
    11041 tttctgcctg tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc
    11101 tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt
    11161 caataccgca gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa
    11221 aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga
    11281 gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc
    11341 gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggccg
    11401 cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc
    11461 gccagcccag cggcgagggc aaccagcccg gtgagcgtcg caaaggagat cctgatctga
    11521 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
    11581 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
    11641 acacattgcg gacgttttta atgtactggg gtggatgcag tgggccccac
//
