LOCUS       sequence-252945-       11960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 11960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH69
FEATURES             Location/Qualifiers
     source          1..11960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2023..2523,2524..2619,2620..3117,3118..3213)
                     /codon_start=1
                     /product="adenine base editor comprising wild-type E. coli 
                     TadA fused to the evolved TadA*(7.10) mutant (Gaudelli et 
                     al., 2017)"
                     /label=ABE(7.10)
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGW
                     NRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVF
                     GARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQ
                     SSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGSSEVEFSHEYWMRHALTLAKRARD
                     EREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVT
                     FEPCVMCAGAMIHSRIGRVVFGVRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECA
                     ALLCYFFRMPRQVFNAQKKAQSSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             join(3214..6612,6613..6615,6616..7212,7213,7214..7216,
                     7217..7218,7219..7221,7222..7314)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3214..3237,3238..3240,3241..7314)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7327..7347
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7363..7615
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7663..7687
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(7843..8991)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(9327..9915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9407..9426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10086..10877,10878..10946))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10709..10728
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10947..11051)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11138..11769
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    11861..11885
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtgcagc gtgacccggt cgtgcccctc
       61 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
      121 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
      181 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
      241 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
      301 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
      361 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
      421 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
      481 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
      541 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
      601 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
      661 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
      721 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
      781 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg
      841 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      901 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      961 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
     1021 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1081 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1141 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1201 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1261 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1321 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1381 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct
     1441 ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt
     1501 gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact
     1561 gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg
     1621 gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt
     1681 aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg
     1741 aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga
     1801 tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac
     1861 aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct
     1921 atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt
     1981 ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gaatgtccga agtcgagttt
     2041 tcccatgagt actggatgag acacgcattg actctcgcaa agagggcttg ggatgaacgc
     2101 gaggtgcccg tgggggcagt actcgtgcat aacaatcgcg taatcggcga aggttggaat
     2161 aggccgatcg gacgccacga ccccactgca catgcggaaa tcatggccct tcgacaggga
     2221 gggcttgtga tgcagaatta tcgacttatc gatgcgacgc tgtacgtcac gcttgaacct
     2281 tgcgtaatgt gcgcgggagc tatgattcac tcccgcattg gacgagttgt attcggtgcc
     2341 cgcgacgcca agacgggtgc cgcaggttca ctgatggacg tgctgcatca cccaggcatg
     2401 aaccaccggg tagaaatcac agaaggcata ttggcggacg aatgtgcggc gctgttgtcc
     2461 gacttttttc gcatgcggag gcaggagatc aaggcccaga aaaaagcaca atcctctact
     2521 gactctggtg gttcttctgg tggttctagc ggcagcgaga ctcccgggac ctcagagtcc
     2581 gccacacccg aaagttctgg tggttcttct ggtggttctt ccgaagtcga gttttcccat
     2641 gagtactgga tgagacacgc attgactctc gcaaagaggg ctcgagatga acgcgaggtg
     2701 cccgtggggg cagtactcgt gctcaacaat cgcgtaatcg gcgaaggttg gaatagggca
     2761 atcggactcc acgaccccac tgcacatgcg gaaatcatgg cccttcgaca gggagggctt
     2821 gtgatgcaga attatcgact tatcgatgcg acgctgtacg tcacgtttga accttgcgta
     2881 atgtgcgcgg gagctatgat tcactcccgc attggacgag ttgtattcgg tgttcgcaac
     2941 gccaagacgg gtgccgcagg ttcactgatg gacgtgctgc attacccagg catgaaccac
     3001 cgggtagaaa tcacagaagg catattggcg gacgaatgtg cggcgctgtt gtgttacttt
     3061 tttcgcatgc ccaggcaggt ctttaacgcc cagaaaaaag cacaatcctc tactgactct
     3121 ggtggttctt ctggtggttc tagcggcagc gagactcccg ggacctcaga gtccgccaca
     3181 cccgaaagtt ctggtggttc ttctggtggt tctgataaaa agtattctat tggtttagcc
     3241 atcggcacta attccgttgg atgggctgtc ataaccgatg aatacaaagt accttcaaag
     3301 aaatttaagg tgttggggaa cacagaccgt cattcgatta aaaagaatct tatcggtgcc
     3361 ctcctattcg atagtggcga aacggcagag gcgactcgcc tgaaacgaac cgctcggaga
     3421 aggtatacac gtcgcaagaa ccgaatatgt tacttacaag aaatttttag caatgagatg
     3481 gccaaagttg acgattcttt ctttcaccgt ttggaagagt ccttccttgt cgaagaggac
     3541 aagaaacatg aacggcaccc catctttgga aacatagtag atgaggtggc atatcatgaa
     3601 aagtacccaa cgatttatca cctcagaaaa aagctagttg actcaactga taaagcggac
     3661 ctgaggttaa tctacttggc tcttgcccat atgataaagt tccgtgggca ctttctcatt
     3721 gagggtgatc taaatccgga caactcggat gtcgacaaac tgttcatcca gttagtacaa
     3781 acctataatc agttgtttga agagaaccct ataaatgcaa gtggcgtgga tgcgaaggct
     3841 attcttagcg cccgcctctc taaatcccga cggctagaaa acctgatcgc acaattaccc
     3901 ggagagaaga aaaatgggtt gttcggtaac cttatagcgc tctcactagg cctgacacca
     3961 aattttaagt cgaacttcga cttagctgaa gatgccaaat tgcagcttag taaggacacg
     4021 tacgatgacg atctcgacaa tctactggca caaattggag atcagtatgc ggacttattt
     4081 ttggctgcca aaaaccttag cgatgcaatc ctcctatctg acatactgag agttaatact
     4141 gagattacca aggcgccgtt atccgcttca atgatcaaaa ggtacgatga acatcaccaa
     4201 gacttgacac ttctcaaggc cctagtccgt cagcaactgc ctgagaaata taaggaaata
     4261 ttctttgatc agtcgaaaaa cgggtacgca ggttatattg acggcggagc gagtcaagag
     4321 gaattctaca agtttatcaa acccatatta gagaagatgg atgggacgga agagttgctt
     4381 gtaaaactca atcgcgaaga tctactgcga aagcagcgga ctttcgacaa cggtagcatt
     4441 ccacatcaaa tccacttagg cgaattgcat gctatactta gaaggcagga ggatttttat
     4501 ccgttcctca aagacaatcg tgaaaagatt gagaaaatcc taacctttcg cataccttac
     4561 tatgtgggac ccctggcccg agggaactct cggttcgcat ggatgacaag aaagtccgaa
     4621 gaaacgatta ctccatggaa ttttgaggaa gttgtcgata aaggtgcgtc agctcaatcg
     4681 ttcatcgaga ggatgaccaa ctttgacaag aatttaccga acgaaaaagt attgcctaag
     4741 cacagtttac tttacgagta tttcacagtg tacaatgaac tcacgaaagt taagtatgtc
     4801 actgagggca tgcgtaaacc cgcctttcta agcggagaac agaagaaagc aatagtagat
     4861 ctgttattca agaccaaccg caaagtgaca gttaagcaat tgaaagagga ctactttaag
     4921 aaaattgaat gcttcgattc tgtcgagatc tccggggtag aagatcgatt taatgcgtca
     4981 cttggtacgt atcatgacct cctaaagata attaaagata aggacttcct ggataacgaa
     5041 gagaatgaag atatcttaga agatatagtg ttgactctta ccctctttga agatcgggaa
     5101 atgattgagg aaagactaaa aacatacgct cacctgttcg acgataaggt tatgaaacag
     5161 ttaaagaggc gtcgctatac gggctgggga cgattgtcgc ggaaacttat caacgggata
     5221 agagacaagc aaagtggtaa aactattctc gattttctaa agagcgacgg cttcgccaat
     5281 aggaacttta tgcagctgat ccatgatgac tctttaacct tcaaagagga tatacaaaag
     5341 gcacaggttt ccggacaagg ggactcattg cacgaacata ttgcgaatct tgctggttcg
     5401 ccagccatca aaaagggcat actccagaca gtcaaagtag tggatgagct agttaaggtc
     5461 atgggacgtc acaaaccgga aaacattgta atcgagatgg cacgcgaaaa tcaaacgact
     5521 cagaaggggc aaaaaaacag tcgagagcgg atgaagagaa tagaagaggg tattaaagaa
     5581 ctgggcagcc agatcttaaa ggagcatcct gtggaaaata cccaattgca gaacgagaaa
     5641 ctttacctct attacctaca aaatggaagg gacatgtatg ttgatcagga actggacata
     5701 aaccgtttat ctgattacga cgtcgatcac attgtacccc aatccttttt gaaggacgat
     5761 tcaatcgaca ataaagtgct tacacgctcg gataagaacc gagggaaaag tgacaatgtt
     5821 ccaagcgagg aagtcgtaaa gaaaatgaag aactattggc ggcagctcct aaatgcgaaa
     5881 ctgataacgc aaagaaagtt cgataactta actaaagctg agaggggtgg cttgtctgaa
     5941 cttgacaagg ccggatttat taaacgtcag ctcgtggaaa cccgccaaat cacaaagcat
     6001 gttgcacaga tactagattc ccgaatgaat acgaaatacg acgagaacga taagctgatt
     6061 cgggaagtca aagtaatcac tttaaagtca aaattggtgt cggacttcag aaaggatttt
     6121 caattctata aagttaggga gataaataac taccaccatg cgcacgacgc ttatcttaat
     6181 gccgtcgtag ggaccgcact cattaagaaa tacccgaagc tagaaagtga gtttgtgtat
     6241 ggtgattaca aagtttatga cgtccgtaag atgatcgcga aaagcgaaca ggagataggc
     6301 aaggctacag ccaaatactt cttttattct aacattatga atttctttaa gacggaaatc
     6361 actctggcaa acggagagat acgcaaacga cctttaattg aaaccaatgg ggagacaggt
     6421 gaaatcgtat gggataaggg ccgggacttc gcgacggtga gaaaagtttt gtccatgccc
     6481 caagtcaaca tagtaaagaa aactgaggtg cagaccggag ggttttcaaa ggaatcgatt
     6541 cttccaaaaa ggaatagtga taagctcatc gctcgtaaaa aggactggga cccgaaaaag
     6601 tacggtggct tcgatagccc tacagttgcc tattctgtcc tagtagtggc aaaagttgag
     6661 aagggaaaat ccaagaaact gaagtcagtc aaagaattat tggggataac gattatggag
     6721 cgctcgtctt ttgaaaagaa ccccatcgac ttccttgagg cgaaaggtta caaggaagta
     6781 aaaaaggatc tcataattaa actaccaaag tatagtctgt ttgagttaga aaatggccga
     6841 aaacggatgt tggctagcgc cggagagctt caaaagggga acgaactcgc actaccgtct
     6901 aaatacgtga atttcctgta tttagcgtcc cattacgaga agttgaaagg ttcacctgaa
     6961 gataacgaac agaagcaact ttttgttgag cagcacaaac attatctcga cgaaatcata
     7021 gagcaaattt cggaattcag taagagagtc atcctagctg atgccaatct ggacaaagta
     7081 ttaagcgcat acaacaagca cagggataaa cccatacgtg agcaggcgga aaatattatc
     7141 catttgttta ctcttaccaa cctcggcgct ccagccgcat tcaagtattt tgacacaacg
     7201 atagatcgca aacgatacac ttctaccaag gaggtgctag acgcgacact gattcaccaa
     7261 tccatcacgg gattatatga aactcggata gatttgtcac agcttggggg tgactctggt
     7321 ggttctccca agaagaagag gaaagtctaa gcttgtcaag cagatcgttc aaacatttgg
     7381 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     7441 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     7501 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     7561 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgacgc
     7621 tactattgtc ttctgcacga agtggtttaa actatcagtg tttgacagga tatattggcg
     7681 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     7741 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc cagatcaggc
     7801 gctggctgct gaacccccag ccggaactga ccccacaagg ccctagcgtt tgcaatgcac
     7861 caggtcatca ttgacccagg cgtgttccac caggccgctg cctcgcaact cttcgcaggc
     7921 ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc acatgaggcg
     7981 gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg
     8041 tcgggccgtc ggcgacagct tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc
     8101 agcaaacagc acgacgattt cctcgtcgat caggacctgg caacgggacg ttttcttgcc
     8161 acggtccagg acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc
     8221 ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta
     8281 ataccggcca ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat
     8341 cggctcgccg ataggggtgc gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc
     8401 atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa
     8461 aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc
     8521 agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa
     8581 caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt
     8641 ggcctcgctg acctgttttg ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat
     8701 agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct gttcaagacg
     8761 acgcgaacgc tccacggcgg ccgatggcgc gggcagggca gggggagcca gttgcacgct
     8821 gtcgcgctcg atcttggccg tagcttgctg gaccatcgag ccgacggact ggaaggtttc
     8881 gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc
     8941 cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc
     9001 ttgcgggatt gccccgactc acgccggggc aatgtgccct tattcctgat ttgacccgcc
     9061 tggtgccttg gtgtccagat aatccacctt atcggcaatg aagtcggtcc cgtagaccgt
     9121 ctggccgtcc ttctcgtact tggtattccg aatcttgccc tgcacgaata ccagcgaccc
     9181 cttgcccaaa tacttgccgt gggcctcggc ctgagagcca aaacacttga tgcggaagaa
     9241 gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac atctaggatc tgccaggaac
     9301 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9961 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10021 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10081 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10141 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10201 ggccccagtg ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca
    10261 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10321 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10381 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10441 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10501 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10561 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10621 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10681 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10741 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10801 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10861 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10921 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10981 caaggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11041 ggggttccgc gcacgaattg gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg
    11101 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa
    11161 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa
    11221 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa
    11281 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc
    11341 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct
    11401 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt
    11461 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct
    11521 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg
    11581 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt
    11641 tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt
    11701 tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg
    11761 gtgagcgtcg caaaggagat cctgatctga ctgatgggct gcctgtatcg agtggtgatt
    11821 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg
    11881 taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta atgtactggg
    11941 gtggatgcag tgggccccac
//
