LOCUS       sequence-252946-       10327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10327)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 10327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH54
FEATURES             Location/Qualifiers
     source          1..10327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1362..5465
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1362..5465
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5478..5498
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6030..6054
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6210..7358)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(7694..8282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7774..7793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8453..9244,9245..9313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9076..9095
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9314..9418)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9505..10136
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10228..10252
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtcgagc tgcaggtcaa cggatcagga
       61 tattcttgtt taagatgttg aactctatgg aggtttgtat gaactgatga tctaggaccg
      121 gataagttcc cttcttcata gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt
      181 acattgttat taatgaaaaa atattattgg tcattggact gaacacgagt gttaaatatg
      241 gaccaggccc caaataagat ccattgatat atgaattaaa taacaagaat aaatcgagtc
      301 accaaaccac ttgccttttt taacgagact tgttcaccaa cttgatacaa aagtcattat
      361 cctatgcaaa tcaataatca tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg
      421 gataatttca caatatgtta tacgataaag aagttacttt tccaagaaat tcactgattt
      481 tataagccca cttgcattag ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc
      541 acgaagaatt ctagaaaata cgaaatacgc ttcaatgcag tgggacccac ggttcaatta
      601 ttgccaattt tcagctccac cgtatattta aaaaataaaa cgataatgct aaaaaaatat
      661 aaatcgtaac gatcgttaaa tctcaacggc tggatcttat gacgaccgtt agaaattgtg
      721 gttgtcgacg agtcagtaat aaacggcgtc aaagtggttg cagccggcac acacgagtcg
      781 tgtttatcaa ctcaaagcac aaatactttt cctcaaccta aaaataaggc aattagccaa
      841 aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca ttttattatt agctattgct
      901 tcaccgcctt agctttctcg tgacctagtc gtcctcgtct tttcttcttc ttcttctata
      961 aaacaatacc caaagagctc ttcttcttca caattcagat ttcaatttct caaaatctta
     1021 aaaactttct ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc
     1081 tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg tatatgttct ttggtttaga
     1141 ttctgttaat cttagatcga acacgatttt ctgggtttga tcgttagata tcatcttaat
     1201 tctcgattag ggtttcatag atatcatccg atttgttcaa ataatttgag ttttgtcgaa
     1261 taattactct tcgatttgtg atttctatct agatctggtg ttagtttcta gtttgtgcga
     1321 tcgaatttgt cgattaatct gagtttttct gattaacagg aatggataag aagtactcta
     1381 tcggactcga tatcggaact aactctgtgg gatgggctgt gatcaccgat gagtacaagg
     1441 tgccatctaa gaagttcaag gttctcggaa acaccgatag gcactctatc aagaaaaacc
     1501 ttatcggtgc tctcctcttc gattctggtg aaactgctga ggctaccaga ctcaagagaa
     1561 ccgctagaag aaggtacacc agaagaaaga acaggatctg ctacctccaa gagatcttct
     1621 ctaacgagat ggctaaagtg gatgattcat tcttccacag gctcgaagag tcattcctcg
     1681 tggaagaaga taagaagcac gagaggcacc ctatcttcgg aaacatcgtt gatgaggtgg
     1741 cataccacga gaagtaccct actatctacc acctcagaaa gaagctcgtt gattctactg
     1801 ataaggctga tctcaggctc atctacctcg ctctcgctca catgatcaag ttcagaggac
     1861 acttcctcat cgagggtgat ctcaaccctg ataactctga tgtggataag ttgttcatcc
     1921 agctcgtgca gacctacaac cagcttttcg aagagaaccc tatcaacgct tcaggtgtgg
     1981 atgctaaggc tatcctctct gctaggctct ctaagtcaag aaggcttgag aacctcattg
     2041 ctcagctccc tggtgagaag aagaacggac ttttcggaaa cttgatcgct ctctctctcg
     2101 gactcacccc taacttcaag tctaacttcg atctcgctga ggatgcaaag ctccagctct
     2161 caaaggatac ctacgatgat gatctcgata acctcctcgc tcagatcgga gatcagtacg
     2221 ctgatttgtt cctcgctgct aagaacctct ctgatgctat cctcctcagt gatatcctca
     2281 gagtgaacac cgagatcacc aaggctccac tctcagcttc tatgatcaag agatacgatg
     2341 agcaccacca ggatctcaca cttctcaagg ctcttgttag acagcagctc ccagagaagt
     2401 acaaagagat tttcttcgat cagtctaaga acggatacgc tggttacatc gatggtggtg
     2461 catctcaaga agagttctac aagttcatca agcctatcct cgagaagatg gatggaaccg
     2521 aggaactcct cgtgaagctc aatagagagg atcttctcag aaagcagagg accttcgata
     2581 acggatctat ccctcatcag atccacctcg gagagttgca cgctatcctt agaaggcaag
     2641 aggatttcta cccattcctc aaggataaca gggaaaagat tgagaagatt ctcaccttca
     2701 gaatccctta ctacgtggga cctctcgcta gaggaaactc aagattcgct tggatgacca
     2761 gaaagtctga ggaaaccatc accccttgga acttcgaaga ggtggtggat aagggtgcta
     2821 gtgctcagtc tttcatcgag aggatgacca acttcgataa gaaccttcca aacgagaagg
     2881 tgctccctaa gcactctttg ctctacgagt acttcaccgt gtacaacgag ttgaccaagg
     2941 ttaagtacgt gaccgaggga atgaggaagc ctgctttttt gtcaggtgag caaaagaagg
     3001 ctatcgttga tctcttgttc aagaccaaca gaaaggtgac cgtgaagcag ctcaaagagg
     3061 attacttcaa gaaaatcgag tgcttcgatt cagttgagat ttctggtgtt gaggataggt
     3121 tcaacgcatc tctcggaacc taccacgatc tcctcaagat cattaaggat aaggatttct
     3181 tggataacga ggaaaacgag gatatcttgg aggatatcgt tcttaccctc accctctttg
     3241 aagatagaga gatgattgaa gaaaggctca agacctacgc tcatctcttc gatgataagg
     3301 tgatgaagca gttgaagaga agaagataca ctggttgggg aaggctctca agaaagctca
     3361 ttaacggaat cagggataag cagtctggaa agacaatcct tgatttcctc aagtctgatg
     3421 gattcgctaa cagaaacttc atgcagctca tccacgatga ttctctcacc tttaaagagg
     3481 atatccagaa ggctcaggtt tcaggacagg gtgatagtct ccatgagcat atcgctaacc
     3541 tcgctggatc tcctgcaatc aagaagggaa tcctccagac tgtgaaggtt gtggatgagt
     3601 tggtgaaggt gatgggaagg cataagcctg agaacatcgt gatcgaaatg gctagagaga
     3661 accagaccac tcagaaggga cagaagaact ctagggaaag gatgaagagg atcgaggaag
     3721 gtatcaaaga gcttggatct cagatcctca aagagcaccc tgttgagaac actcagctcc
     3781 agaatgagaa gctctacctc tactacctcc agaacggaag ggatatgtat gtggatcaag
     3841 agttggatat caacaggctc tctgattacg atgttgatca tatcgtgcca cagtcattct
     3901 tgaaggatga ttctatcgat aacaaggtgc tcaccaggtc tgataagaac aggggtaaga
     3961 gtgataacgt gccaagtgaa gaggttgtga agaaaatgaa gaactattgg aggcagctcc
     4021 tcaacgctaa gctcatcact cagagaaagt tcgataactt gactaaggct gagaggggag
     4081 gactctctga attggataag gcaggattca tcaagaggca gcttgtggaa accaggcaga
     4141 tcactaagca cgttgcacag atcctcgatt ctaggatgaa caccaagtac gatgagaacg
     4201 ataagttgat cagggaagtg aaggttatca ccctcaagtc aaagctcgtg tctgatttca
     4261 gaaaggattt ccaattctac aaggtgaggg aaatcaacaa ctaccaccac gctcacgatg
     4321 cttaccttaa cgctgttgtt ggaaccgctc tcatcaagaa gtatcctaag ctcgagtcag
     4381 agttcgtgta cggtgattac aaggtgtacg atgtgaggaa gatgatcgct aagtctgagc
     4441 aagagatcgg aaaggctacc gctaagtatt tcttctactc taacatcatg aatttcttca
     4501 agaccgagat taccctcgct aacggtgaga tcagaaagag gccactcatc gagacaaacg
     4561 gtgaaacagg tgagatcgtg tgggataagg gaagggattt cgctaccgtt agaaaggtgc
     4621 tctctatgcc acaggtgaac atcgttaaga aaaccgaggt gcagaccggt ggattctcta
     4681 aagagtctat cctccctaag aggaactctg ataagctcat tgctaggaag aaggattggg
     4741 accctaagaa atacggtggt ttcgattctc ctaccgtggc ttactctgtt ctcgttgtgg
     4801 ctaaggttga gaagggaaag agtaagaagc tcaagtctgt taaggaactt ctcggaatca
     4861 ctatcatgga aaggtcatct ttcgagaaga acccaatcga tttcctcgag gctaagggat
     4921 acaaagaggt taagaaggat ctcatcatca agctcccaaa gtactcactc ttcgaactcg
     4981 agaacggtag aaagaggatg ctcgcttctg ctggtgagct tcaaaaggga aacgagcttg
     5041 ctctcccatc taagtacgtt aactttcttt acctcgcttc tcactacgag aagttgaagg
     5101 gatctccaga agataacgag cagaagcaac ttttcgttga gcagcacaag cactacttgg
     5161 atgagatcat cgagcagatc tctgagttct ctaaaagggt gatcctcgct gatgcaaacc
     5221 tcgataaggt gttgtctgct tacaacaagc acagagataa gcctatcagg gaacaggcag
     5281 agaacatcat ccatctcttc acccttacca acctcggtgc tcctgctgct ttcaagtact
     5341 tcgatacaac catcgatagg aagagataca cctctaccaa agaagtgctc gatgctaccc
     5401 tcatccatca gtctatcact ggactctacg agactaggat cgatctctca cagctcggtg
     5461 gtgattcaag ggctgatcct aagaagaaga ggaaggtttg agctttccag gcctcccagc
     5521 tttcgtccgt atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc
     5581 ccacacacca gaatcctact aagtttgagt attatggcat tggaaaagct gttttcttct
     5641 atcatttgtt ctgcttgtaa tttactgtgt tctttcagtt tttgttttcg gacatcaaaa
     5701 tgcaaatgga tggataagag ttaataaatg atatggtcct tttgttcatt ctcaaattat
     5761 tattatctgt tgtttttact ttaatgggtt gaatttaagt aagaaaggaa ctaacagtgt
     5821 gatattaagg tgcaatgtta gacatataaa acagtctttc acctctcttt ggttatgtct
     5881 tgaattggtt tgtttcttca cttatctgtg taatcaagtt tactatgagt ctatgatcaa
     5941 gtaattatgc aatcaagtta agtacagtat aggcttgagc tccccgctac tattgtcttc
     6001 tgcacgaagt ggtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
     6061 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     6121 cgtccatttg tatgtgcatg ccaaccacag ggttccccag atcaggcgct ggctgctgaa
     6181 cccccagccg gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg
     6241 acccaggcgt gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc
     6301 tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc
     6361 ttgagcgggt acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc
     6421 gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg
     6481 acgatttcct cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg
     6541 cggaagcggt gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc
     6601 atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg
     6661 atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata
     6721 ggggtgcgct tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc
     6781 agctcgacgc cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt
     6841 tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg
     6901 tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc
     6961 atttccttga tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc
     7021 tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg
     7081 tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt caagacgacg cgaacgctcc
     7141 acggcggccg atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc
     7201 ttggccgtag cttgctggac catcgagccg acggactgga aggtttcgcg gggcgcacgc
     7261 atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt
     7321 tcttgcctgt atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc
     7381 ccgactcacg ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg
     7441 tccagataat ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc
     7501 tcgtacttgg tattccgaat cttgccctgc acgaatacca gcgacccctt gcccaaatac
     7561 ttgccgtggg cctcggcctg agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc
     7621 tgcttgtcgc cggcatcgtt gcgccacatc taggatctgc caggaaccgt aaaaaggccg
     7681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7861 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8101 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8221 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8281 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8341 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8401 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8461 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8521 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8581 caatgatacc gcgagaacca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8641 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8701 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8761 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8821 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8881 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8941 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9001 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9061 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9121 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9181 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9241 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9301 gttgaatact catactcttc ctttttcaat attattgaag catttatcaa ggttattgtc
     9361 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9421 cgaattggcc agcgctgcca tttttggggt gaggccgttc gcggccgagg ggcgcagccc
     9481 ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg ggggcacccc
     9541 ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa
     9601 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac
     9661 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc
     9721 ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag
     9781 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct
     9841 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg
     9901 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc
     9961 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc
    10021 cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg
    10081 cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggtg agcgtcgcaa
    10141 aggagatcct gatctgactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
    10201 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
    10261 gcttagacaa cttaataaca cattgcggac gtttttaatg tactggggtg gatgcagtgg
    10321 gccccac
//
