LOCUS       sequence-252950-        9827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH58
FEATURES             Location/Qualifiers
     source          1..9827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2023..5181
                     /codon_start=1
                     /product="Cas9 endonuclease from the Staphylococcus aureus 
                     Type II CRISPR/Cas system"
                     /label=SaCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MKRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNE
                     GRRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEE
                     FSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGE
                     VRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFG
                     WKDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKF
                     QIIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKE
                     IIENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKA
                     INLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSI
                     KVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENA
                     KYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVK
                     QEENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRF
                     SVQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKER
                     NKGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEI
                     FITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKD
                     NDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKK
                     DNGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKN
                     LDVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLL
                     NRIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQ
                     IIKKG"
     CDS             5194..5214
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5230..5482
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5530..5554
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(5710..6858)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(7194..7782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7274..7293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7953..8744,8745..8813))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8576..8595
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8814..8918)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9005..9636
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    9728..9752
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtgcagc gtgacccggt cgtgcccctc
       61 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
      121 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
      181 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
      241 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
      301 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
      361 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
      421 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
      481 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
      541 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
      601 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
      661 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
      721 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
      781 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg
      841 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      901 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      961 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
     1021 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1081 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1141 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1201 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1261 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1321 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1381 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct
     1441 ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt
     1501 gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact
     1561 gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg
     1621 gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt
     1681 aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg
     1741 aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga
     1801 tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac
     1861 aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct
     1921 atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt
     1981 ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gaatgaagag gaactacatc
     2041 ctcggactcg atatcggaat cacctctgtg ggatacggaa tcatcgatta cgagactagg
     2101 gatgtgatcg atgctggtgt gagactcttc aaagaggcta acgttgagaa caacgaggga
     2161 agaaggtcta agaggggagc tagaaggctc aagagaagaa gaaggcacag aatccagagg
     2221 gtgaagaagc tcctcttcga ttacaacctc ctcaccgatc actctgagct ttctggaatc
     2281 aacccttacg aggcaagagt gaagggactc tctcagaagt tgtctgagga agagttctct
     2341 gctgctttgc tccaccttgc taagagaagg ggtgtgcata acgtgaacga ggtggaagag
     2401 gataccggaa acgagctttc taccaaagag cagatctcta ggaactctaa ggctctcgaa
     2461 gagaagtacg tggcagagct tcagcttgag agactcaaga aagatggtga ggtgagggga
     2521 tctatcaaca ggttcaagac ctctgattac gtgaaagaag ctaagcagct cctcaaggtg
     2581 cagaaggctt accatcagct cgatcagtct ttcatcgata cctacatcga tcttctcgag
     2641 actagaagga cctactacga gggacctggt gaaggatctc cattcggatg gaaggatatc
     2701 aaagaatggt acgagatgct catgggacac tgcacttact tccctgaaga actcagatct
     2761 gtgaagtacg cttacaacgc tgatctctac aacgctctta acgatctcaa caacctcgtg
     2821 atcaccaggg atgagaacga gaagcttgag tactacgaga agttccagat catcgagaac
     2881 gtgttcaagc aaaagaagaa gcctaccctc aagcagatcg ctaaagagat ccttgttaac
     2941 gaggaagata tcaagggata cagggtgacc tctaccggaa agcctgagtt cactaacctc
     3001 aaggtttacc acgatattaa ggatattacc gctagaaaag agattattga gaacgctgag
     3061 cttctcgatc aaatcgctaa gatccttacc atctaccagt catctgagga tatccaagag
     3121 gaattgacca acctcaactc agagcttacc caagaagaga tcgagcagat cagtaacttg
     3181 aagggttaca ccggaaccca caacctctca ctcaaggcta tcaacctcat cctcgatgag
     3241 ctttggcaca ccaacgataa ccagatcgca atcttcaaca gactcaagct cgtgcctaag
     3301 aaagtggatc tctctcagca gaaagagatt cctaccaccc tcgtggatga tttcatcctc
     3361 tcacctgttg tgaagagatc tttcatccag tctatcaagg tgatcaacgc tattatcaag
     3421 aaatacggac tccctaacga tatcatcatc gagttggcta gggaaaagaa ctcaaaggat
     3481 gctcaaaaga tgatcaacga gatgcagaag agaaacaggc agaccaacga gaggatcgaa
     3541 gagatcatca ggaccaccgg aaaagagaac gctaagtacc tcattgagaa gatcaagctc
     3601 cacgatatgc aagagggaaa gtgcctctac tctctcgagg ctatccctct tgaggatctc
     3661 cttaacaacc ctttcaacta cgaagtggat cacatcatcc ctagatctgt gtctttcgat
     3721 aactctttca acaacaaggt gctcgtgaag caagaggaaa actctaagaa gggtaacagg
     3781 acccctttcc agtacctctc ttcatctgat tctaagatct catacgagac tttcaagaag
     3841 cacatcctca acctcgctaa gggaaagggt aggatctcta agacaaagaa agagtacctt
     3901 ctcgaggaaa gggatatcaa cagattctct gttcagaagg atttcatcaa cagaaacctt
     3961 gtggatacca ggtacgctac caggggtctt atgaaccttc tcaggtctta cttcagggtg
     4021 aacaaccttg atgtgaaggt gaagtcaatc aacggtggat tcacctcatt ccttagaaga
     4081 aaatggaagt ttaagaaaga gaggaacaag ggttataagc accacgctga ggatgctctc
     4141 attatcgcta acgcagattt cattttcaaa gagtggaaga agttggataa ggctaaaaag
     4201 gtgatggaaa accagatgtt cgaggaaaag caggctgagt ctatgcctga gatcgagact
     4261 gagcaagagt acaaagaaat cttcatcacc cctcaccaga tcaagcacat caaagatttc
     4321 aaggattaca agtactctca cagagtggat aagaagccaa acagagagct tatcaacgat
     4381 accctctact caaccagaaa ggatgataag ggaaacaccc tcatcgtgaa caatctcaac
     4441 ggactctacg ataaggataa cgataagctc aagaagctca ttaacaagtc tccagagaag
     4501 ttgctcatgt accaccacga tcctcagacc taccaaaagc tcaagctcat catggaacag
     4561 tacggtgatg agaagaaccc tctctacaag tattacgagg aaaccggtaa ctacctcacc
     4621 aagtactcaa aaaaggataa tggacctgtg atcaagaaga ttaagtacta cggaaacaag
     4681 ctcaacgcac acctcgatat caccgatgat taccctaaca gtagaaacaa ggtggtgaag
     4741 ctttcactca agccttacag gttcgatgtg tacctcgata acggtgtgta caagttcgtg
     4801 accgtgaaga acctcgatgt tatcaaaaaa gaaaactact atgaggttaa ctctaagtgc
     4861 tacgaggaag ctaagaagtt gaagaagatc tctaaccagg cagagttcat tgcttcattc
     4921 tacaacaacg atcttatcaa gattaacggt gagctttaca gggttatcgg agtgaacaac
     4981 gatttgctca acaggatcga ggtgaacatg attgatatca cctacaggga atacctcgag
     5041 aatatgaacg ataagaggcc tcctagaatc atcaagacta tcgcttctaa gacccagagt
     5101 atcaagaagt actctaccga tatcctcgga aacctctacg aggttaagtc aaagaagcac
     5161 cctcagatca ttaagaaggg atcaagggct gatcctaaga agaagaggaa ggtttgagct
     5221 tgtcaagcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     5281 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     5341 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     5401 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     5461 gtgtcatcta tgttactaga tcgacgctac tattgtcttc tgcacgaagt ggtttaaact
     5521 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     5581 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     5641 ccaaccacag ggttccccag atcaggcgct ggctgctgaa cccccagccg gaactgaccc
     5701 cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag
     5761 gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg
     5821 ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg
     5881 gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc
     5941 ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag
     6001 gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga
     6061 caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg
     6121 cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg
     6181 gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc
     6241 caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt
     6301 gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat
     6361 tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat
     6421 cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt
     6481 cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc
     6541 ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc
     6601 caaacctgcc gcctcctgtt caagacgacg cgaacgctcc acggcggccg atggcgcggg
     6661 cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac
     6721 catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat
     6781 ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg
     6841 gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat
     6901 gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc
     6961 ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat
     7021 cttgccctgc acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg
     7081 agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt
     7141 gcgccacatc taggatctgc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7201 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7261 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7321 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7381 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7441 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7501 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7561 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7621 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7681 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7741 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7801 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7861 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7921 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7981 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8041 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaacca
     8101 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8161 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8221 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8281 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8341 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8401 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8461 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8521 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8581 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8641 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8701 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8761 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8821 ctttttcaat attattgaag catttatcaa ggttattgtc tcatgagcgg atacatattt
     8881 gaatgtattt agaaaaataa acaaataggg gttccgcgca cgaattggcc agcgctgcca
     8941 tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg
     9001 cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac
     9061 gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt
     9121 taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt
     9181 ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc
     9241 ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa
     9301 taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat
     9361 caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc
     9421 tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc
     9481 cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg
     9541 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc
     9601 agcccagcgg cgagggcaac cagcccggtg agcgtcgcaa aggagatcct gatctgactg
     9661 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
     9721 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
     9781 cattgcggac gtttttaatg tactggggtg gatgcagtgg gccccac
//
