LOCUS       sequence-252961-        2412 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Chu LF, Mamott D, Ni Z, Bacher R, Liu C, Swanson S, Kendziorski C, 
            Stewart R, Thomson JA
  TITLE     An In Vitro Human Segmentation Clock Model Derived from Embryonic 
            Stem Cells.
  JOURNAL   Cell Rep. 2019 Aug 27;28(9):2247-2255.e5. doi: 
            10.1016/j.celrep.2019.07.090.
  PUBMED    31461642
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-27- 27"; volume: "28"; issue: "9"; pages: "2247-2255.e5"
COMMENT     Sequence Label: pB-TAG-NICD
FEATURES             Location/Qualifiers
     source          1..2412
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtgctgc tgtcccgcaa gcgccggcgg cagcatggcc agctctggtt ccctgagggc
       61 ttcaaagtgt ctgaggccag caagaagaag cggcgggagc ccctcggcga ggactccgtg
      121 ggcctcaagc ccctgaagaa cgcttcagac ggtgccctca tggacgacaa ccagaatgag
      181 tggggggacg aggacctgga gaccaagaag ttccggttcg aggagcccgt ggttctgcct
      241 gacctggacg accagacaga ccaccggcag tggactcagc agcacctgga tgccgctgac
      301 ctgcgcatgt ctgccatggc ccccacaccg ccccagggtg aggttgacgc cgactgcatg
      361 gacgtcaatg tccgcgggcc tgatggcttc accccgctca tgatcgcctc ctgcagcggg
      421 ggcggcctgg agacgggcaa cagcgaggaa gaggaggacg cgccggccgt catctccgac
      481 ttcatctacc agggcgccag cctgcacaac cagacagacc gcacgggcga gaccgccttg
      541 cacctggccg cccgctactc acgctctgat gccgccaagc gcctgctgga ggccagcgca
      601 gatgccaaca tccaggacaa catgggccgc accccgctgc atgcggctgt gtctgccgac
      661 gcacaaggtg tcttccagat cctgatccgg aaccgagcca cagacctgga tgcccgcatg
      721 catgatggca cgacgccact gatcctggct gcccgcctgg ccgtggaggg catgctggag
      781 gacctcatca actcacacgc cgacgtcaac gccgtagatg acctgggcaa gtccgccctg
      841 cactgggccg ccgccgtgaa caatgtggat gccgcagttg tgctcctgaa gaacggggct
      901 aacaaagata tgcagaacaa cagggaggag acacccctgt ttctggccgc ccgggagggc
      961 agctacgaga ccgccaaggt gctgctggac cactttgcca accgggacat cacggatcat
     1021 atggaccgcc tgccgcgcga catcgcacag gagcgcatgc atcacgacat cgtgaggctg
     1081 ctggacgagt acaacctggt gcgcagcccg cagctgcacg gagccccgct ggggggcacg
     1141 cccaccctgt cgcccccgct ctgctcgccc aacggctacc tgggcagcct caagcccggc
     1201 gtgcagggca agaaggtccg caagcccagc agcaaaggcc tggcctgtgg aagcaaggag
     1261 gccaaggacc tcaaggcacg gaggaagaag tcccaggacg gcaagggctg cctgctggac
     1321 agctccggca tgctctcgcc cgtggactcc ctggagtcac cccatggcta cctgtcagac
     1381 gtggcctcgc cgccactgct gccctccccg ttccagcagt ctccgtccgt gcccctcaac
     1441 cacctgcctg ggatgcccga cacccacctg ggcatcgggc acctgaacgt ggcggccaag
     1501 cccgagatgg cggcgctggg tgggggcggc cggctggcct ttgagactgg cccacctcgt
     1561 ctctcccacc tgcctgtggc ctctggcacc agcaccgtcc tgggctccag cagcggaggg
     1621 gccctgaatt tcactgtggg cgggtccacc agtttgaatg gtcaatgcga gtggctgtcc
     1681 cggctgcaga gcggcatggt gccgaaccaa tacaaccctc tgcgggggag tgtggcacca
     1741 ggccccctga gcacacaggc cccctccctg cagcatggca tggtaggccc gctgcacagt
     1801 agccttgctg ccagcgccct gtcccagatg atgagctacc agggcctgcc cagcacccgg
     1861 ctggccaccc agcctcacct ggtgcagacc cagcaggtgc agccacaaaa cttacagatg
     1921 cagcagcaga acctgcagcc agcaaacatc cagcagcagc aaagcctgca gccgccacca
     1981 ccaccaccac agccgcacct tggcgtgagc tcagcagcca gcggccacct gggccggagc
     2041 ttcctgagtg gagagccgag ccaggcagac gtgcagccac tgggccccag cagcctggcg
     2101 gtgcacacta ttctgcccca ggagagcccc gccctgccca cgtcgctgcc atcctcgctg
     2161 gtcccacccg tgaccgcagc ccagttcctg acgcccccct cgcagcacag ctactcctcg
     2221 cctgtggaca acacccccag ccaccagcta caggtgcctg agcacccctt cctcaccccg
     2281 tcccctgagt cccctgacca gtggtccagc tcgtccccgc attccaacgt ctccgactgg
     2341 tccgagggcg tctccagccc tcccaccagc atgcagtccc agatcgcccg cattccggag
     2401 gccttcaagt aa
//
