LOCUS       sequence-252966-       11731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of RET G533D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-RET-G533D
FEATURES             Location/Qualifiers
     source          1..11731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6668..6692)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6745..6786
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6807..7374
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6824..7376
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6968..6985)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7195..7214
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7388..7390,7391..7393,7394..8107)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7388..7390,7391..7393,7394..8104)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7433..7454)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7694..7713)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    8121..8709
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8174..8194)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8592..8603)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8784..9017
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9136..9154)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9222..9326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9327..9395,9396..10187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9545..9564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10358..10946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10847..10866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11100..11117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11357..11378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11393..11398,11399..11416,11417..11423)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11431..11447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11436..11458
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11455..11471
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11455..11471
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11521..11540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gcgaaggcga cgtccggtgc cgcggggctg
     3481 cgtctgctgt tgctgctgct gctgccgctg ctaggcaaag tggcattggg cctctacttc
     3541 tcgagggatg cttactggga gaagctgtat gtggaccagg cggccggcac gcccttgctg
     3601 tacgtccatg ccctgcggga cgcccctgag gaggtgccca gcttccgcct gggccagcat
     3661 ctctacggca cgtaccgcac acggctgcat gagaacaact ggatctgcat ccaggaggac
     3721 accggcctcc tctaccttaa ccggagcctg gaccatagct cctgggagaa gctcagtgtc
     3781 cgcaaccgcg gctttcccct gctcaccgtc tacctcaagg tcttcctgtc acccacatcc
     3841 cttcgtgagg gcgagtgcca gtggccaggc tgtgcccgcg tatacttctc cttcttcaac
     3901 acctcctttc cagcctgcag ctccctcaag ccccgggagc tctgcttccc agagacaagg
     3961 ccctccttcc gcattcggga gaaccgaccc ccaggcacct tccaccagtt ccgcctgctg
     4021 cctgtgcagt tcttgtgccc caacatcagc gtggcctaca ggctcctgga gggtgagggt
     4081 ctgcccttcc gctgcgcccc ggacagcctg gaggtgagca cgcgctgggc cctggaccgc
     4141 gagcagcggg agaagtacga gctggtggcc gtgtgcaccg tgcacgccgg cgcgcgcgag
     4201 gaggtggtga tggtgccctt cccggtgacc gtgtacgacg aggacgactc ggcgcccacc
     4261 ttccccgcgg gcgtcgacac cgccagcgcc gtggtggagt tcaagcggaa ggaggacacc
     4321 gtggtggcca cgctgcgtgt cttcgatgca gacgtggtac ctgcatcagg ggagctggtg
     4381 aggcggtaca caagcacgct gctccccggg gacacctggg cccagcagac cttccgggtg
     4441 gaacactggc ccaacgagac ctcggtccag gccaacggca gcttcgtgcg ggcgaccgta
     4501 catgactata ggctggttct caaccggaac ctctccatct cggagaaccg caccatgcag
     4561 ctggcggtgc tggtcaatga ctcagacttc cagggcccag gagcgggcgt cctcttgctc
     4621 cacttcaacg tgtcggtgct gccggtcagc ctgcacctgc ccagtaccta ctccctctcc
     4681 gtgagcagga gggctcgccg atttgcccag atcgggaaag tctgtgtgga aaactgccag
     4741 gcattcagtg gcatcaacgt ccagtacaag ctgcattcct ctggtgccaa ctgcagcacg
     4801 ctaggggtgg tcacctcagc cgaggacacc tcggggatcc tgtttgtgaa tgacaccaag
     4861 gccctgcggc ggcccaagtg tgccgaactt cactacatgg tggtggccac cgaccagcag
     4921 acctctaggc aggcccaggc ccagctgctt gtaacagtgg aggggtcata tgtggccgag
     4981 gaggcgggct gccccctgtc ctgtgcagtc agcaagagac ggctggagtg tgaggagtgt
     5041 ggcgacctgg gctccccaac aggcaggtgt gagtggaggc aaggagatgg caaagggatc
     5101 accaggaact tctccacctg ctctcccagc accaagacct gccccgacgg ccactgcgat
     5161 gttgtggaga cccaagacat caacatttgc cctcaggact gcctccgggg cagcattgtt
     5221 gggggacacg agcctgggga gccccggggg attaaagctg gctatggcac ctgcaactgc
     5281 ttccctgagg aggagaagtg cttctgcgag cccgaagaca tccaggatcc actgtgcgac
     5341 gagctgtgcc gcacggtgat cgcagccgct gtcctcttct ccttcatcgt ctcggtgctg
     5401 ctgtctgcct tctgcatcca ctgctaccac aagtttgccc acaagccacc catctcctca
     5461 gctgagatga ccttccggag gcccgcccag gccttcccgg tcagctactc ctcttccagt
     5521 gcccgccggc cctcgctgga ctccatggag aaccaggtct ccgtggatgc cttcaagatc
     5581 ctggaggatc caaagtggga attccctcgg aagaacttgg ttcttggaaa aactctagga
     5641 gaaggcgaat ttggaaaagt ggtcaaggca acggccttcc atctgaaagg cagagcaggg
     5701 tacaccacgg tggccgtgaa gatgctgaaa gagaacgcct ccccgagtga gcttcgagac
     5761 ctgctgtcag agttcaacgt cctgaagcag gtcaaccacc cacatgtcat caaattgtat
     5821 ggggcctgca gccaggatgg cccgctcctc ctcatcgtgg agtacgccaa atacggctcc
     5881 ctgcggggct tcctccgcga gagccgcaaa gtggggcctg gctacctggg cagtggaggc
     5941 agccgcaact ccagctccct ggaccacccg gatgagcggg ccctcaccat gggcgacctc
     6001 atctcatttg cctggcagat ctcacagggg atgcagtatc tggccgagat gaagctcgtt
     6061 catcgggact tggcagccag aaacatcctg gtagctgagg ggcggaagat gaagatttcg
     6121 gatttcggct tgtcccgaga tgtttatgaa gaggattcgt acgtgaagag gagccagggt
     6181 cggattccag ttaaatggat ggcaattgaa tccctttttg atcatatcta caccacgcaa
     6241 agtgatgtat ggtcttttgg tgtcctgctg tgggagatcg tgaccctagg gggaaacccc
     6301 tatcctggga ttcctcctga gcggctcttc aaccttctga agaccggcca ccggatggag
     6361 aggccagaca actgcagcga ggagatgtac tgcctgatgc tgcaatgctg gaagcaggag
     6421 ccggacaaaa ggccggtgtt tgcggacatc agcaaagacc tggagaagat gatggttaag
     6481 aggagagact acttggacct tgcggcgtcc actccatctg actccctgat ttatgacgac
     6541 ggcctctcag aggaggagac accgctggtg gactgtaata atgcccccct ccctcgagcc
     6601 ctcccttcca catggattga aaacaaactc tatggtagaa tttcccatgc atttactaga
     6661 ttctagaacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
     6721 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     6781 tctacgcgta ccggttagta atgagatccc tccccccccc ctaacgttac tggccgaagc
     6841 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     6901 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     6961 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     7021 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     7081 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     7141 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     7201 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     7261 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     7321 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     7381 cacacatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     7441 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     7501 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     7561 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     7621 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     7681 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     7741 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     7801 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     7861 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     7921 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     7981 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     8041 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     8101 caagtaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
     8161 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     8221 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     8281 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     8341 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     8401 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     8461 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     8521 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     8581 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     8641 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     8701 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8761 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     8821 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8881 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8941 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9001 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     9061 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt
     9121 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     9181 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     9301 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     9361 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     9421 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     9481 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     9541 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     9601 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9661 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9721 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9781 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9841 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9901 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9961 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    10021 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    10081 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    10141 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    10201 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    10261 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    10321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10381 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    10441 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10501 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10561 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10621 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10681 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10741 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    10801 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10861 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10921 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10981 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11041 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    11101 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    11161 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
    11221 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    11281 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
    11341 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    11401 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    11461 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
    11521 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    11581 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    11641 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    11701 tttgttaaat atgtactaca aacttagtag t
//
