LOCUS       sequence-252975-        3738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone encoding wild-type human ATG3 with a stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Agrotis A, von Chamier L, Oliver H, Kiso K, Singh T, Ketteler R
  TITLE     Human ATG4 autophagy proteases counteract attachment of 
            ubiquitin-like LC3/GABARAP proteins to other cellular proteins.
  JOURNAL   J Biol Chem. 2019 Jul 17. pii: AC119.009977. doi: 
            10.1074/jbc.AC119.009977.
  PUBMED    31315929
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2019 Jul 17. pii: AC119.009977. doi: 
            10.1074/jbc.AC119.009977."
COMMENT     Sequence Label: pDONR223 ATG3 WT
FEATURES             Location/Qualifiers
     source          1..3738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     297..313
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     297..313
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     318..337
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        318..336
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    354..453
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1402..1501)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1517..1534)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1517..1533)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1526..1548)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1597..1683
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1775..1802
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1961..1978)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2132..2720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2212..2231)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2813..3604)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
       61 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg
      121 cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct
      181 tgggtagcag cgaagtcgag gcatttctgt cctggctggt ctagaggagg catgatgata
      241 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacgggccag agctgccagg
      301 aaacagctat gaccatgtaa tacgactcac tataggggat atcagctgga tggcaaataa
      361 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttc
      421 ttataatgcc aactttgtac aagaaagctg ggtattacat tgtgaagtgt cttgtgtagt
      481 catattctat tgttggaatg acagcttgta caaatttcaa gaaaataaga agatacatat
      541 gaactccaag ttctccccct ccttctgcaa cagtctcaat gattttcttc atcacctcag
      601 catgcctgca tgggtgaact gaacacatgg gaggtggtgg cagatgaggg tgattttcaa
      661 tggtcactgt tttcttcaca tgatcctgac tgatgtcttc atacatgtgc tcaactgtta
      721 aaggctgccg ttgctcatca tagccaaaca accataatcg tggagtctgg taatatttat
      781 cataagtgat gtaaaggtca taagttctgg tttgcaaaat agcatcttca ccgccagcat
      841 cagttttggc tttacaagct tctactattt tccttgtatc tagggtagcc tcatctgttt
      901 ccaacaatcc actctcttca tattcttcca tatctgcagc ttctccttca tcttcatctt
      961 cttcctcttc acatagtgct gagcaatctt gaagccttat attgtcctta ttttccagtg
     1021 tgatctcttt aacggcttcc gttattcctg taatacctgt gttgtgatat gtatctaccc
     1081 atccgccatc accatcatct tcttcaatga tagcttccaa ttcatctgaa tattccatct
     1141 gtttgcaccg cttatagcac ggcacatttt tggttaccaa aaattgtttg cctgttggta
     1201 ggtatgcctt cactttcaat tcttcccctg tagcccattg ccatgttgga cagtggtgga
     1261 ctaggtgatc tccagctgcc acaaactctt ctggggtaat tacacctgtt tccttaaact
     1321 ttgattcctt gaggaccggg gtcaggtact cagccacttc cagtgccttt cccttcacag
     1381 tattaatcac attctgcatt aagcctgctt ttttgtacaa agttggcatt ataaaaaagc
     1441 attgctcatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1501 ggggcccgag cttaagactg gccgtcgttt tacaacgtcg tgactgggaa aacatccatg
     1561 ctagcgttaa cgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
     1621 gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     1681 aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggagggtg gcgggcagga
     1741 cgcccgccat aaactgccag gcatcaaact aagcagaagg ccatcctgac ggatggcctt
     1801 tttgcgtttc tacaaactct tcctggctag cggtacgcgt attaattgcg ttgcgctcac
     1861 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1921 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1981 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2041 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2101 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2161 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2221 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2281 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2341 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2401 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2461 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2521 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2581 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2641 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2701 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2761 ggaacgacgc gtaactcacg ttaagggatt ttggtcatgg gtggctcgag ggttatttgc
     2821 cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc
     2881 gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg
     2941 gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga
     3001 ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat
     3061 cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata
     3121 gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc
     3181 tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga
     3241 agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg
     3301 gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa
     3361 tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg
     3421 ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca
     3481 ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc
     3541 gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc
     3601 tcataatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc
     3661 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     3721 actgtacccc aaaaaaac
//
