LOCUS       sequence-252981-       11350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ROR1 T69M.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11350)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ROR1-T69M
FEATURES             Location/Qualifiers
     source          1..11350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6287..6311)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6364..6405
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6426..6993
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6443..6995
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6587..6604)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6814..6833
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7007..7009,7010..7012,7013..7726)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7007..7009,7010..7012,7013..7723)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7052..7073)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7313..7332)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7660..7681
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7740..8328
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7793..7813)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8211..8222)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8403..8636
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8755..8773)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8841..8945
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8946..9014,9015..9806)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9164..9183)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9977..10565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10466..10485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10719..10736
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10976..10997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11012..11017,11018..11035,11036..11042)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11050..11066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11055..11077
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11074..11090
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11074..11090
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11140..11159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc agaagggccg tcaaggccca ccatgcaccg gccgcgccgc
     3481 cgcgggacgc gcccgccgct cctggcgctg ctggccgcgc tgctgctggc cgcacgcggg
     3541 gctgctgccc aagaaacaga gctgtcagtc agtgctgaat tagtgcctac ctcatcatgg
     3601 aacatctcaa gtgaactcaa caaagattct tacctgaccc tcgatgaacc aatgaataac
     3661 atcaccatgt ctctgggcca gacagcagaa ctgcactgca aagtctctgg gaatccacct
     3721 cccaccatcc gctggttcaa aaatgatgct cctgtggtcc aggagccccg gaggctctcc
     3781 tttcggtcca ccatctatgg ctctcggctg cggattagaa acctcgacac cacagacaca
     3841 ggctacttcc agtgcgtggc aacaaacggc aaggaggtgg tttcttccac tggagtcttg
     3901 tttgtcaagt ttggcccccc tcccactgca agtccaggat actcagatga gtatgaagaa
     3961 gatggattct gtcagccata cagagggatt gcatgtgcaa gatttattgg caaccgcacc
     4021 gtctatatgg agtctttgca catgcaaggg gaaatagaaa atcagatcac agctgccttc
     4081 actatgattg gcacttccag tcacttatct gataagtgtt ctcagttcgc cattccttcc
     4141 ctgtgccact atgccttccc gtactgcgat gaaacttcat ccgtcccaaa gccccgtgac
     4201 ttgtgtcgcg atgaatgtga aatcctggag aatgtcctgt gtcaaacaga gtacattttt
     4261 gcaagatcaa atcccatgat tctgatgagg ctgaaactgc caaactgtga agatctcccc
     4321 cagccagaga gcccagaagc tgcgaactgt atccggattg gaattcccat ggcagatcct
     4381 ataaataaaa atcacaagtg ttataacagc acaggtgtgg actaccgggg gaccgtcagt
     4441 gtgaccaaat cagggcgcca gtgccagcca tggaattccc agtatcccca cacacacact
     4501 ttcaccgccc ttcgtttccc agagctgaat ggaggccatt cctactgccg caacccaggg
     4561 aatcaaaagg aagctccctg gtgcttcacc ttggatgaaa actttaagtc tgatctgtgt
     4621 gacatcccag cgtgcgattc aaaggattcc aaggagaaga ataaaatgga aatcctgtac
     4681 atactagtgc caagtgtggc cattcccctg gccattgctt tactcttctt cttcatttgc
     4741 gtctgtcgga ataaccagaa gtcatcgtcg gcaccagtcc agaggcaacc aaaacacgtc
     4801 agaggtcaaa atgtagagat gtcaatgctg aatgcatata aacccaagag caaggctaaa
     4861 gagctacctc tttctgctgt acgctttatg gaagaattgg gtgagtgtgc ctttggaaaa
     4921 atctataaag gccatctcta tctcccaggc atggaccatg ctcagctggt tgctatcaag
     4981 accttgaaag actataacaa cccccagcaa tggacggaat ttcaacaaga agcctcccta
     5041 atggcagaac tgcaccaccc caatattgtc tgccttctag gtgccgtcac tcaggaacaa
     5101 cctgtgtgca tgctttttga gtatattaat cagggggatc tccatgagtt cctcatcatg
     5161 agatccccac actctgatgt tggctgcagc agtgatgaag atgggactgt gaaatccagc
     5221 ctggaccacg gagattttct gcacattgca attcagattg cagctggcat ggaatacctg
     5281 tctagtcact tctttgtcca caaggacctt gcagctcgca atattttaat cggagagcaa
     5341 cttcatgtaa agatttcaga cttggggctt tccagagaaa tttactccgc tgattactac
     5401 agggtccaga gtaagtcctt gctgcccatt cgctggatgc cccctgaagc catcatgtat
     5461 ggcaaattct cttctgattc agatatctgg tcctttgggg ttgtcttgtg ggagattttc
     5521 agttttggac tccagccata ttatggattc agtaaccagg aagtgattga gatggtgaga
     5581 aaacggcagc tcttaccatg ctctgaagac tgcccaccca gaatgtacag cctcatgaca
     5641 gagtgctgga atgagattcc ttctaggaga ccaagattta aagatattca cgtccggctt
     5701 cggtcctggg agggactctc aagtcacaca agctctacta ctccttcagg gggaaatgcc
     5761 accacacaga caacctccct cagtgccagc ccagtgagta atctcagtaa ccccagatat
     5821 cctaattaca tgttcccgag ccagggtatt acaccacagg gccagattgc tggtttcatt
     5881 ggcccgccaa tacctcagaa ccagcgattc attcccatca atggataccc aatacctcct
     5941 ggatatgcag cgtttccagc tgcccactac cagccaacag gtcctcccag agtgattcag
     6001 cactgcccac ctcccaagag tcggtcccca agcagtgcca gtgggtcgac tagcactggc
     6061 catgtgacta gcttgccctc atcaggatcc aatcaggaag caaatattcc tttactacca
     6121 cacatgtcaa ttccaaatca tcctggtgga atgggtatca ccgtttttgg caacaaatct
     6181 caaaaaccct acaaaattga ctcaaagcaa gcatctttac taggagacgc caatattcat
     6241 ggacacaccg aatctatgat ttctgcagaa ctgtaaggcc tcatgggccc agctttcttg
     6301 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc
     6361 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa
     6421 tgagatccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     6481 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     6541 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     6601 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     6661 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     6721 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     6781 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     6841 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     6901 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     6961 gacgtggttt tcctttgaaa aacacgatga taatatggcc acacatatgg tgagcaaggg
     7021 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     7081 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     7141 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     7201 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     7261 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     7321 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     7381 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     7441 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     7501 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     7561 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     7621 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     7681 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaatcg atagatccta
     7741 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     7801 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     7861 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     7921 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     7981 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     8041 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     8101 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     8161 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     8221 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     8281 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt
     8341 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     8401 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     8461 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8521 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8581 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     8641 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     8701 tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg
     8761 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     8821 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8881 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8941 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9001 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9061 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9121 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9181 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9241 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9301 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9361 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9421 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9481 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9541 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9601 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9661 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9721 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9781 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9841 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9901 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9961 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10021 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10081 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10141 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10201 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10261 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10321 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10381 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10441 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10501 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10561 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10621 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10681 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10741 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10801 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
    10861 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    10921 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    10981 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11041 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
    11101 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    11161 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    11221 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
    11281 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    11341 acttagtagt
//
