LOCUS       sequence-253-exp        3611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4119
FEATURES             Location/Qualifiers
     source          1..3611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..41
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     25..41
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(725..741)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(726..742)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(766..785)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(767..785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(795..812)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(795..811)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(804..826)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      953..1408
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1040..1059)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1250..1271
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1434..1538
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1539..1607,1608..2399)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1757..1776)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2570..3158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3059..3078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3312..3329
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3446..3467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3482..3487,3488..3505,3506..3512)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3520..3536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3525..3547
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3544..3560
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3544..3560
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3579..3599
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3581..3599
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctccacc gcggtggcgg ccgctctaga actagtggat ccaccggtgc tccactatta
       61 gaggatacgg aatgctgtgg aaaagagaga tgtcagcatg cgactgaaga atctgaatac
      121 aagaaacttg tatatgtagc tgtgcatctt gtgaatgtac tcgaacgact atctcttcct
      181 tctgcacata tcgatgattc cgatgaggaa aaagagccga aagccgacgt tcgaagactg
      241 atgatcgtgt gccatgaatt acatattcca cttctacatc atacacttga agagaggatt
      301 cctgatagta acagtgcaga gttgggactt cccaaagctg cagttttgga acaacttcta
      361 gaacttgacc tagatgtgaa tgccactgat aagaataacg acactccaat gcacattctg
      421 cttcgagcca gagagtttag gaaaagcttg gttcgagcac tgctcgtacg tggcacatgg
      481 ctgttcgcaa ggaatcggca cggagatgtg gtgttgaatg tgatgaaacg tatgaaagcg
      541 ttgaatcacg ccaactttga tgatctacca ctaggtgcgt gtcgaatcag aaattgagag
      601 ttattagatt atttggaacg cgaataactt cattatacca cggagagagc cagaaattga
      661 agaaattggt taaggagaat ggaatttaat gaataatagc gcaccaattc gatatcaagc
      721 ttatcgatac cgtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg
      781 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      841 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      901 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
      961 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     1021 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     1081 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     1141 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     1201 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     1261 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     1321 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     1381 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     1441 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1501 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1561 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1621 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1681 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1741 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1801 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1861 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1921 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1981 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2041 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2101 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2161 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2221 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2281 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2341 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2401 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2461 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2521 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2581 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2641 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2701 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2761 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2821 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2881 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2941 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3001 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3061 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3121 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3181 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3241 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3301 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3361 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3421 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     3481 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     3541 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     3601 aacaaaagct g
//
