LOCUS       sequence-25300-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Hageman J, Kampinga HH
  TITLE     Computational analysis of the human HSPH/HSPA/DNAJ family and 
            cloning of a human HSPH/HSPA/DNAJ expression library.
  JOURNAL   Cell Stress Chaperones. 2009 Jan;14(1):1-21. doi: 
            10.1007/s12192-008-0060-2. Epub 2008 Aug 7.
  PUBMED    18686016
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12192-008-0060-2"; journalName: "Cell Stress Chaperones"; 
            date: "2009-01"; volume: "14"; issue: "1"; pages: "1-21"
COMMENT     Sequence Label: pcDNA5/FRT/TO V5 DNAJB2a
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..124
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     RBS             271..279
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa
       61 acttaagctt gaattcacca tgggtaagcc tatccctaac cctctcctcg gtctcgattc
      121 tacgggatcc accatggcat cctactacga gatcctagac gtgccgcgaa gtgcgtccgc
      181 tgatgacatc aagaaggcgt atcggcgcaa ggctctccag tggcacccag acaaaaaccc
      241 agataataaa gagtttgctg agaagaaatt taaggaggtg gccgaggcat atgaagtgct
      301 gtctgacaag cacaagcggg agatttacga ccgctatggc cgggaagggc tgacagggac
      361 aggaactggc ccatctcggg cagaagctgg cagtggtggg cctggcttca ccttcacctt
      421 ccgcagcccc gaggaggtct tccgggaatt ctttgggagt ggagaccctt ttgcagagct
      481 ctttgatgac ctgggcccct tctcagagct tcagaaccgg ggttcccgac actcaggccc
      541 cttctttacc ttctcttcct ccttccctgg gcactccgat ttctcctcct catctttcnn
      601 nnngnngngn gggggntggt gcttttcgct ctnnnnnnna catctaccac ctttgtccaa
      661 ggacgccgca tcaccacacg cagaatcatg gnnaacgggc nnnncgggtg nagtgaggag
      721 nngggcagct gantcnntcn natcat
//
