LOCUS       sequence-253016-        3435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 RNA Pol for in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Wu RA, Collins K
  TITLE     Human telomerase specialization for repeat synthesis by unique 
            handling of primer-template duplex.
  JOURNAL   EMBO J. 2014 Apr 16;33(8):921-35. doi: 10.1002/embj.201387205. Epub 
            2014 Mar 11.
  PUBMED    24619002
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/embj.201387205"; journalName: "EMBO J"; date: "2014-04-16- 
            16"; volume: "33"; issue: "8"; pages: "921-35"
COMMENT     Sequence Label: pUC119 T7 hTRmin
FEATURES             Location/Qualifiers
     source          1..3435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    23..44
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(59..64,65..82,83..89)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    97..113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     102..124
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     121..137
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     121..137
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     156..175
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        156..174
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(480..497)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(480..496)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(489..511)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      709..1164
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(796..815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1006..1027
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1181..1200)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1300..1322
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1360..1378)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1446..1550
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1551..1619,1620..2411)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1769..1788)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2582..3170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3071..3090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3324..3341
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
       61 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      121 caggaaacag ctatgaccat gattacgcca agctttaata cgactcacta tagggcctgg
      181 gaggggtggt ggccattttt tgtctaaccc taactgagaa gggcgtaggc gccgtgcttt
      241 tgctccccgc gcgctgtttt tctcgctgac tttcagcggg cggaaaagcc tcggcctgcc
      301 gccttccacc gttcattcta gagcaaacaa aaaatgtcag ctgctggccc gttcgcccct
      361 cccggggagg atccaataat aataataacc ccgcctggag gccgcggtcg gcccggggct
      421 tctccggagg cacccactgc caccgcgaag agttgggctc tgtcagccgc ggggaattca
      481 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      541 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      601 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
      661 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
      721 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      781 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      841 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
      901 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
      961 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     1021 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
     1081 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     1141 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     1201 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     1261 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1321 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     1381 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     1441 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1501 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1561 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1621 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1681 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1741 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     1801 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1861 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1921 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1981 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2041 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     2101 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2161 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2221 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2281 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2341 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2401 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2461 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2521 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2581 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2641 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2701 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2761 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2821 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2881 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2941 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3001 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3061 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3121 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3181 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3241 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3301 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3361 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3421 ttcccgactg gaaag
//
