LOCUS       sequence-253033-        6106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6106)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-CD36-C-10
FEATURES             Location/Qualifiers
     source          1..6106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        111..414
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        415..618
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     569..589
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      657..666
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             663..1337
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     polyA_signal    2944..3065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2981..3000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3035..3054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3072..3527)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3209..3230)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3421..3440
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3554..3658
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3660..4017
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3660..3851
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3683..3703)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3868..4003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4052..4846
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4052..4846
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4106..4125)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4716..4735
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5034..5053)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5078..5125
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5454..6042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5943..5962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa
       61 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      121 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      181 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      241 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      301 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      361 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      421 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      481 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      541 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      601 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca
      661 ccatggtgag tgtgattaaa ccagacatga agatcaagct gcgtatggaa ggcgctgtaa
      721 atggacaccc gttcgcgatt gaaggagttg gccttgggaa gcctttcgag ggaaaacaga
      781 gtatggacct taaagtcaaa gaaggcggac ctctgccttt cgcctatgac atcttgacaa
      841 ctgtgttctg ttacggcaac agggtattcg ccaaataccc agaaaatata gtagactatt
      901 tcaagcagtc gtttcctgag ggctactctt gggaacgaag catgaattac gaagacgggg
      961 gcatttgtaa cgcgacaaac gacataaccc tggatggtga ctgttatatc tatgaaattc
     1021 gatttgatgg tgtgaacttt cctgccaatg gtccagttat gcagaagagg actgtgaaat
     1081 gggagccatc cactgagaaa ttgtatgtgc gtgatggagt gctgaagggt gatgttaaca
     1141 tggctctgtc gcttgaagga ggtggccatt accgatgtga cttcaaaact acttataaag
     1201 ctaagaaggt tgtccagttg ccagactatc actttgtgga ccaccacatt gagattaaaa
     1261 gccacgacaa agattacagt aatgttaatc tgcatgagca cgccgaagcg cattctgagc
     1321 tgccgaggca ggccaagtcc ggactcagat ctggcagcag tggaggtggc tgtgaccgga
     1381 actgtgggct catcgctggg gctgtcattg gtgctgtcct ggctgtgttt ggaggtattc
     1441 taatgccagt tggagacctg cttatccaga agacaattaa aaagcaagtt gtcctcgaag
     1501 aaggtacaat tgcttttaaa aattgggtta aaacaggcac agaagtttac agacagtttt
     1561 ggatctttga tgtgcaaaat ccacaggaag tgatgatgaa cagcagcaac attcaagtta
     1621 agcaaagagg tccttatacg tacagagttc gttttctagc caaggaaaat gtaacccagg
     1681 acgctgagga caacacagtc tctttcctgc agcccaatgg tgccatcttc gaaccttcac
     1741 tatcagttgg aacagaggct gacaacttca cagttctcaa tctggctgtg gcagctgcat
     1801 cccatatcta tcaaaatcaa tttgttcaaa tgatcctcaa ttcacttatt aacaagtcaa
     1861 aatcttctat gttccaagtc agaactttga gagaactgtt atggggctat agggatccat
     1921 ttttgagttt ggttccgtac cctgttacta ccacagttgg tctgttttat ccttacaaca
     1981 atactgcaga tggagtttat aaagttttca atggaaaaga taacataagt aaagttgcca
     2041 taatcgacac atataaaggt aaaaggaatc tgtcctattg ggaaagtcac tgcgacatga
     2101 ttaatggtac agatgcagcc tcatttccac cttttgttga gaaaagccag gtattgcagt
     2161 tcttttcttc tgatatttgc aggtcaatct atgctgtatt tgaatccgac gttaatctga
     2221 aaggaatccc tgtgtataga tttgttcttc catccaaggc ctttgcctct ccagttgaaa
     2281 acccagacaa ctattgtttc tgcacagaaa aaattatctc aaaaaattgt acatcatatg
     2341 gtgtgctaga catcagcaaa tgcaaagaag ggagacctgt gtacatttca cttcctcatt
     2401 ttctgtatgc aagtcctgat gtttcagaac ctattgatgg attaaaccca aatgaagaag
     2461 aacataggac atacttggat attgaaccta taactggatt cactttacaa tttgcaaaac
     2521 ggctgcaggt caacctattg gtcaagccat cagaaaaaat tcaagtatta aagaatctga
     2581 agaggaacta tattgtgcct attctttggc ttaatgagac tgggaccatt ggtgatgaga
     2641 aggcaaacat gttcagaagt caagtaactg gaaaaataaa cctccttggc ctgatagaaa
     2701 tgatcttact cagtgttggt gtggtgatgt ttgttgcttt tatgatttca tattgtgcat
     2761 gcagatcgaa aacaataaaa taagaattct gcagtcgacg gtaccgcggg cccgggatcc
     2821 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     2881 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     2941 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3001 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3061 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     3121 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3181 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     3241 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     3301 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3361 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3421 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3481 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     3541 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3601 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     3661 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3721 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3781 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3841 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3901 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3961 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     4021 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     4081 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     4141 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     4201 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     4261 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4321 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4381 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4441 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4501 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4561 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     4621 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     4681 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     4741 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     4801 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     4861 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     4921 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     4981 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     5041 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     5101 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     5161 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     5221 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     5281 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     5341 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5401 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5461 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5521 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5581 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5641 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5701 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5761 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5821 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5881 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5941 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6001 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6061 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttct
//
