LOCUS       sequence-253037-        8654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RhoB upstream msfYFP 24xMS2 RhoB downstream for CRISPR tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Wilson MZ, Ravindran PT, Lim WA, Toettcher JE
  TITLE     Tracing Information Flow from Erk to Target Gene Induction Reveals 
            Mechanisms of Dynamic and Combinatorial Control.
  JOURNAL   Mol Cell. 2017 Sep 7;67(5):757-769.e5. doi: 
            10.1016/j.molcel.2017.07.016. Epub 2017 Aug 17.
  PUBMED    28826673
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.07.016"; journalName: "Mol Cell"; date: 
            "2017-09-7- 7"; volume: "67"; issue: "5"; pages: "757-769.e5"
COMMENT     Sequence Label: pAAV RhoB YFP MS2
FEATURES             Location/Qualifiers
     source          1..8654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6162..6617
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6249..6268)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6459..6480
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6634..6653)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6753..6775
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6813..6831)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6899..7003
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7004..7072,7073..7864)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7222..7241)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8035..8623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8524..8543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctggcctttt gctcacatgt agaggtgtca tctcctgcag gaagtggtaa
       61 ttcacgagtt aagacctggt gtgtatgaag aaactccatc ctctgtacag aaaccgtgtg
      121 cccaccggca agttccctgc gaggccgcat gcctgctttc ccagcttgaa gcttttcatc
      181 tgctccatga gagttgttgg gatagatggg tttccttgca cagtgtactt caagcagttc
      241 tctggcttta gggcgagaat cagatgtaat gtggtaggac tgggtgaagg ttgcctgccc
      301 aagctgggtg ctttcttttt cagcggagtg ccctgcacct cgctttcctt tgtcctacag
      361 ctgtgtgtct cctagattcc tccccttatc ttcttcaccc ggagacctaa gtgtgggcct
      421 tgaagagaaa gccgagaggg gactcaccca ggcccacaca atttactgtt agcctttctc
      481 agtgaaaagg ctgccctggg taagtgtcag aattcggccc agagcacacg aggggttagt
      541 tatctaaaca aagttaagaa agaacgaaac ccttggctag gtaacaaaac cgtgcaccgg
      601 tgtctgttta agtgaaaaca aaaccacgtt aaaggcgccg cccctccccc atgcttgatc
      661 acccgtagtt cgctcggaag ctagataaag ctgaagccct gggagcagcc aacgccccac
      721 aagagtgtag ctcgtctcgg aactgcccac ccagacccgg agggctccag gctgcctgct
      781 ccacgatgtc gcactcagag aggctgcgac gaaggcgggg acccggcggc cacacaaccc
      841 cacggggcgg gtcgccgcgc agactccaca gtcaacgcct ctcccagccc cgcggacagg
      901 gcagtcaatc aagctaaggc gaacccgcct tcggactgca gcgggcgacc aatcggagcc
      961 aagctccgca gcgatgagct cagccctatg gcttcccatt gggtggctat attaagaaag
     1021 tggccggact ctttaaatag cgggcgctag ggccgcagcc cgcatctgcc accgcagttc
     1081 ggttgtcgtc gtctgcgccc ctgtacccta ggtctagagt gaacccgccc cgggaaagaa
     1141 gctaggccgg gtccgcagca tcagccatca cttcgcacaa agcagcgcag ctccgggacc
     1201 gccgaggacc acagcggcgc ggcagcggcg cggcgacact cagtgcaccg tatgcccctg
     1261 cgcccctgcc gaggcaaccg gagcgccccg agagaccgcg ccgccgcggg gtccaggtgc
     1321 agttagcgag cctagcccgc agcgcgcagt cgcgggagag cggggagcgg caagcaacag
     1381 ggagcgggac ggcggcgagg cgctcgcggg cccctcctgc tgcccgcgcc cggcgagctc
     1441 atggcggcca tccgcaagaa gctggtggtg gtgggcgacg gcgcgtgcgg caagacgtgc
     1501 ctgctgatcg tgttcagtaa agacgaattc cccgaggtgt acgtgcccac cgtgttcgag
     1561 aactatgtgg cggacatcga ggtggacggc aagcaggtgg agctggcgct gtgggacacg
     1621 gcaggccagg aggactacga tcgtttacgg ccgctctcct atccggacac cgacgtcatc
     1681 cttatgtgct tctcggtgga cagcccggac tctctcgaga acatccccga gaagtgggtg
     1741 cccgaggtaa agcacttctg ccccaatgtg cccatcatcc tggtggccaa caaaaaagac
     1801 ctgcgcagcg acgagcatgt ccgcacggag ctggcccgca tgaagcagga gccagtgcgc
     1861 acggatgacg gccgcgccat ggcggtgcgc atccaagcct atgactacct cgagtgctcg
     1921 gccaagacca aggagggcgt gcgcgaggtt tttgagacgg ccacgcgcgc cgcgctgcag
     1981 aagcgctacg gatcccagaa tggctgcatc aactgctgca aggtgctagg cggatccggg
     2041 ggaagtaaag gtgaagaact gttcaccggt gttgttccga tcctggttga actggatggt
     2101 gatgttaacg gccacaaatt ctctgttcgt ggtgaaggtg aaggtgatgc aaccaacggt
     2161 aaactgaccc tgaaattcat ctgcactacc ggtaaactgc cggttccatg gccgactctg
     2221 gtgactaccc tgacctatgg tgttcagtgt ttttctcgtt acccggatca catgaagcag
     2281 catgatttct tcaaatctgc aatgccggaa ggttatgtac aggagcgcac catttctttc
     2341 aaagacgatg gcacctacaa aacccgtgca gaggttaaat ttgaaggtga tactctggtg
     2401 aaccgtattg aactgaaagg cattgatttc aaagaggacg gcaacatcct gggccacaaa
     2461 ctggaatata acttcaactc ccataacgtt tacatcaccg cagacaaaca gaagaacggt
     2521 atcaaagcta acttcaaaat tcgccataac gttgaagacg gtagcgtaca gctggcggac
     2581 cactaccagc agaacactcc gatcggtgat ggtccggttc tgctgccgga taaccactac
     2641 ctgtcctacc agtctaaact gtccaaagac ccgaacgaaa agcgcgacca catggtgctg
     2701 ctggagttcg ttactgcagc aggtatcacg cacggcatgg atgaactcta caaatgataa
     2761 tcgcccttgg atcctacggt acttattgcc aagaaagcac gagcatcagc cgtgcctcca
     2821 ggtcgaatct tcaaacgacg acgatcacgc gtcgctccag tattccaggg ttcatcagat
     2881 cctacggtac ttattgccaa gaaagcacga gcatcagccg tgcctccagg tcgaatcttc
     2941 aaacgacgac gatcacgcgt cgctccagta ttccagggtt catcagatcc tacggtactt
     3001 attgccaaga aagcacgagc atcagccgtg cctccaggtc gaatcttcaa acgacgacga
     3061 tcacgcgtcg ctccagtatt ccagggttca tcagatccta cggtacttat tgccaagaaa
     3121 gcacgagcat cagccgtgcc tccaggtcga atcttcaaac gacgacgatc acgcgtcgct
     3181 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagcatca
     3241 gccgtgcctc caggtcgaat cttcaaacga cgacgatcac gcgtcgctcc agtattccag
     3301 ggttcatcag atcctacggt acttattgcc aagaaagcac gagcatcagc cgtgcctcca
     3361 ggtcgaatct tcaaacgacg acgatcacgc gtcgctccag tattccaggg ttcatcagat
     3421 cctacggtac ttattgccaa gaaagcacga gcatcagccg tgcctccagg tcgaatcttc
     3481 aaacgacgac gatcacgcgt cgctccagta ttccagggtt catcagatcc tacggtactt
     3541 attgccaaga aagcacgagc atcagccgtg cctccaggtc gaatcttcaa acgacgacga
     3601 tcacgcgtcg ctccagtatt ccagggttca tcagatccta cggtacttat tgccaagaaa
     3661 gcacgagcat cagccgtgcc tccaggtcga atcttcaaac gacgacgatc acgcgtcgct
     3721 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagcatca
     3781 gccgtgcctc caggtcgaat cttcaaacga cgacgatcac gcgtcgctcc agtattccag
     3841 ggttcatcag atcctacggt acttattgcc aagaaagcac gagcatcagc cgtgcctcca
     3901 ggtcgaatct tcaaacgacg acgatcacgc gtcgctccag tattccaggg ttcatcagat
     3961 cctacggtac ttattgccaa gaaagcacga gcatcagccg tgcctccagg tcgaatcttc
     4021 aaacgacgac gatcacgcgt cgctccagta ttccagggtt catcagatct aagggcgaat
     4081 tcgtttggga cgcgtcctgc ctcacgccct tgccagcgtg gctccccctc cttggcccgg
     4141 tcgcccacga accgggagaa agggagaccc atgcccccaa ggacaccacc agactgcctg
     4201 acatctgctg gtggctctgg ctggtcacgc tgagaattag cgtgggcacc gagctccccc
     4261 ttcccagtgt ctgtgtgtgt ccagctgtgt ggcacaggcc tgggcaccct gctgagtgcc
     4321 aaggggttcc tgagcatcct tttctaaaga gccaggcctc aaagtgtggt tgtgtgtgtg
     4381 tacgactccc tacaccccac ccgactcctg ccccaccccc gcctctggtt tccccagggg
     4441 cacgcagagt ggttgcgccc ccagcaggtt tgcttgtaac cagcaagcca ctactgttgc
     4501 tccatgtctg taacatagac ccctggaatg gcgggagggg agggctgggg ggaggatggg
     4561 gctgttacat aaatacagat tttattttcg gaggcagaat ggtattgttt agtggtgtgt
     4621 ggggcgacca gggcccagag cagctccttt cccaggctgg gtcaggcgcc cacccatcca
     4681 agcatgaact ggacttggcc atctttccac accctgggga agacatttgc aactgacttg
     4741 gggtcgagag gaagcagctc agacagtgtc tcctgggcca acccccagcg aacctccggc
     4801 agaggatcca gggtgtgcag tggggtcact tttgccataa gcgaactttg tgcctgtcct
     4861 acaaatgaac attgttcagc tcaagagact attgttactg aatttattta aacgctgaag
     4921 ctttttgttg ttgatgaaag agttctttgc acaattgtcc cattgtttga cacccagtgt
     4981 acttgtcatt tgcataagac agcattctga ccacacttgt atgctgtaac ctcatctact
     5041 tctgatggtt tttttaaaac tatgatgact ctaagattac aaaaacaact aacttttgct
     5101 ttgttttctt gaaaaaaaaa tgtcaacagt gtgaattttt tctttaaatt tgtgtagcat
     5161 acgcagtttt ggtaaaggaa ggcaacaggt attggggtct atttaaaccc cccctcccac
     5221 aagacaagtc tcttctgtgt gaaattttct gtacgttcca tgtgcagaga aaagcctccc
     5281 ccctctcccc cccgccagcc cagtggtact tctaaattgt cttgttttgt ttttgtttta
     5341 ttttttttaa ataaactgac agatgacaaa tggtgaactt atgatgttta cataagagtt
     5401 ctataagctg tgtatacaga tttttatgta aaatattaaa agactatgat catgacattt
     5461 ataaacagct cctgtggctt aatagtgtca gaagatgtct gaattggggg taagggaaga
     5521 gttcttgtgg aggtcctttc tggcagtaac tgcagcctct ctatagtagt aaatatggcc
     5581 atgagcctgg caccttttag gggctgggtg accctgagag gccaggttat tcctgtcaga
     5641 ttcccggagg aggctaagct tgtcgtccat cagacaccca gagtgtacta attattgtga
     5701 atttagtccc attaggtgag gacaaaggtc ttggtttgac ttcatctata ggtctttaag
     5761 aagtgtctga agtttattct gggctctact actctcactt caacaataag aaaggttgct
     5821 gtttgtgaat gttaaacata gatgacttta ttattattat ttaaaatgtt tttagggggg
     5881 tggttaatca catgtgtgga ccaggctaga atttaagaga tggggaaagc atggcagtga
     5941 tggcggatgc cttcagtccc agcaaaagca ggcaaatctc ttgagttata ggccagcctg
     6001 gactacagat gcagagtggg ttccagggct acacagagaa aaaagaacag acaagcaaag
     6061 gaatagacaa tactcccaga aactaagggc gcctgatgcg gtattttctc cttacgcatc
     6121 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     6181 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6241 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6301 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6361 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6421 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6481 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     6541 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6601 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     6661 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     6721 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     6781 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     6841 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     6901 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6961 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7021 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7081 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7141 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7201 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7261 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7321 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7381 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7441 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7501 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7561 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7621 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7681 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7741 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7801 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7861 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7921 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7981 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8041 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8101 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8161 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     8221 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8281 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8341 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8401 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8461 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8521 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8581 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8641 ctttttacgg ttcc
//
