LOCUS       sequence-253047-       10705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ace-8aa-mScarlet in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Beck C, Gong Y
  TITLE     A high-speed, bright, red fluorescent voltage sensor to detect 
            neural activity.
  JOURNAL   Sci Rep. 2019 Nov 4;9(1):15878. doi: 10.1038/s41598-019-52370-8.
  PUBMED    31685893
REFERENCE   2  (bases 1 to 10705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-11-4"; volume: "9(1):15878. doi"; pages: " 
            10.1038/s41598-019-52370-8"
COMMENT     Sequence Label: LV-Camk2-Ace-8aa-mScarlet
FEATURES             Location/Qualifiers
     source          1..10705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    189..306
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        351..1639
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      1649..1658
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2475..2493)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2743..2762
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3146..3734
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3199..3219)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3617..3628)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3737..3753)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3738..3754)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4259..4439
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4465..4482)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4471..4695
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4741..5169
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4828..4847)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5038..5059
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5178..5198)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5183..5512
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5363..5498
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5425..5444
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5560..5607
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5626..6000
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6130..6251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6167..6186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6221..6240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6300..6316)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6300..6316)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6313..6335)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6324..6340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6348..6354,6355..6372,6373..6378))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6393..6414
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6531..6548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6702..7290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6782..6801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7461..8252,8253..8321))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8084..8103
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8322..8426)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8501..8520)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8692..9071
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9073..9271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9226..9246
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9289..9469
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9516..9641
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10134..10367
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10552..10596
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
       61 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
      121 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      181 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      241 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      301 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa cattatggcc
      361 ttaggtcact tcatctccat ggggttcttc ttctgatttt ctagaaaatg agatgggggt
      421 gcagagagct tcctcagtga cctgcccagg gtcacatcag aaatgtcaga gctagaactt
      481 gaactcagat tactaatctt aaattccatg ccttgggggc atgcaagtac gatatacaga
      541 aggagtgaac tcattagggc agatgaccaa tgagtttagg aaagaagagt ccagggcagg
      601 gtacatctac accacccgcc cagccctggg tgagtccagc cacgttcacc tcattatagt
      661 tgcctctctc cagtcctacc ttgacgggaa gcacaagcag aaactgggac aggagcccca
      721 ggagaccaaa tcttcatggt ccctctggga ggatgggtgg ggagagctgt ggcagaggcc
      781 tcaggagggg ccctgctgct cagtggtgac agataggggt gagaaagcag acagagtcat
      841 tccgtcagca ttctgggtct gtttggtact tcttctcacg ctaaggtggc ggtgtgatat
      901 gcacaatggc taaaaagcag ggagagctgg aaagaaacaa ggacagagac agaggccaag
      961 tcaaccagac caattcccag aggaagcaaa gaaaccatta cagagactac aagggggaag
     1021 ggaaggagag atgaattagc ttcccctgta aaccttagaa cccagctgtt gccagggcaa
     1081 cggggcaata cctgtctctt cagaggagat gaagttgcca gggtaactac atcctgtctt
     1141 tctcaaggac catcccagaa tgtggcaccc actagccgtt accatagcaa ctgcctcttt
     1201 gccccactta atcccatccc gtctgttaaa agggccctat agttggaggt gggggaggta
     1261 ggaagagcga tgatcacttg tggactaagt ttgttcgcat ccccttctcc aaccccctca
     1321 gtacatcacc ctgggggaac agggtccact tgctcctggg cccacacagt cctgcagtat
     1381 tgtgtatata aggccagggc aaagaggagc aggttttaaa gtgaaaggca ggcaggtgtt
     1441 ggggaggcag ttaccggggc aacgggaaca gggcgtttcg gaggtggttg ccatggggac
     1501 ctggatgctg acgaaggctc gcgaggctgt gagcagccac agtgccctgc tcagaagccc
     1561 caagctcgtc agtcaagccg gttctccgtt tgcactcagg agcacgggca ggcgagtggc
     1621 ccctagttct gggggcagcg ggggatccgc caccatggct gacgtggaaa ccgagaccgg
     1681 catgattgca cagtggattg tctttgctat tatggctgct gctgctattg cttttggagt
     1741 ggctgtgcac tttcggcctt cagagctgaa gagcgcatac tatatcaaca ttgccatctg
     1801 cactatcgcc gctaccgctt actatgcaat ggccgtgaac taccaggacc tgacaatgaa
     1861 tggtgaaagg caggtggtct acgcaagata tattaactgg gtgctgacca caccactgct
     1921 cctgctcgat ctcatcgtca tgaccaagat gggcggagtg atgatttctt gggtcatcgg
     1981 cgcagacatt ttcatgatcg tgtttggtat tctgggcgcc ttcgaggatg aacacaagtt
     2041 caaatgggtg tactttatcg ctggatgtgt gatgcaggca gtcctgacat acgggatgta
     2101 taacgccact tggaaagacg atctgaagaa aagccccgag taccatagct cctatgtcag
     2161 tctgctcgtc ttcctgtcaa tcctctgggt gttttatcct gtcgtgtggg ctttcgggtc
     2221 tggtagtggc gtgctgtccg tcgacaatga ggccattctc atgggaatcc tggatgtgct
     2281 cgctaagcca ctgtttggaa tggggtgcct cattgcccat gagactatct tcaagaaaat
     2341 gctccgaggt agcggactag cagtgatcaa ggagttcatg cggttcaagg tgcacatgga
     2401 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     2461 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tctcctggga
     2521 catcctgtcc cctcagttca tgtacggctc cagggccttc accaagcacc ccgccgacat
     2581 ccccgactac tataagcagt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     2641 cgaggacggc ggcgccgtga ccgtgaccca ggacacctcc ctggaggacg gcaccctgat
     2701 ctacaaggtg aagctccgcg gcaccaactt ccctcctgac ggccccgtaa tgcagaagaa
     2761 gacaatgggc tgggaagcgt ccaccgagcg gttgtacccc gaggacggcg tgctgaaggg
     2821 cgacattaag atggccctgc gcctgaagga cggcggccgc tacctggcgg acttcaagac
     2881 cacctacaag gccaagaagc ccgtgcagat gcccggcgcc tacaacgtcg accgcaagtt
     2941 ggacatcacc tcccacaacg aggactacac cgtggtggaa cagtacgaac gctccgaggg
     3001 ccgccactcc accggcggca tggacgagct gtacaagaag agcaggatca ccagcgaggg
     3061 cgagtacatc cccctggacc agatcgacat caacgtgttc tgctacgaga acgaggtgta
     3121 agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     3181 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3241 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3301 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3361 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3421 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3481 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3541 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gggggacgtc
     3601 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3661 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3721 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     3781 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     3841 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     3901 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     3961 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     4021 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     4081 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     4141 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     4201 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     4261 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4321 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4381 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4441 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     4501 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     4561 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     4621 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     4681 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     4741 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4801 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4861 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4921 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4981 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5041 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5101 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5161 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5221 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5341 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5401 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     5461 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     5521 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     5581 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     5641 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     5701 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     5761 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     5821 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     5881 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     5941 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     6001 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6061 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6121 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6181 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6241 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     6301 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7141 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7321 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7381 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7441 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7501 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7561 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7621 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7681 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7741 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7801 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7861 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7921 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7981 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8041 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8101 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8161 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8221 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8281 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8341 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8401 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     8461 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     8521 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     8581 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     8641 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     8701 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     8761 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     8821 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     8881 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     8941 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     9001 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     9061 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     9121 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     9181 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     9241 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
     9301 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     9361 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     9421 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     9481 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     9541 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     9601 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     9661 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     9721 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     9781 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     9841 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     9901 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     9961 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
    10021 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
    10081 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
    10141 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
    10201 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
    10261 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
    10321 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
    10381 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
    10441 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    10501 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    10561 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    10621 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    10681 gtttttgctg tactttctat agtga
//
