LOCUS       sequence-253049-       10702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ace-7aa-mScarlet in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Beck C, Gong Y
  TITLE     A high-speed, bright, red fluorescent voltage sensor to detect 
            neural activity.
  JOURNAL   Sci Rep. 2019 Nov 4;9(1):15878. doi: 10.1038/s41598-019-52370-8.
  PUBMED    31685893
REFERENCE   2  (bases 1 to 10702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-11-4"; volume: "9(1):15878. doi"; pages: " 
            10.1038/s41598-019-52370-8"
COMMENT     Sequence Label: LV-Camk2-Ace-7aa-mScarlet
FEATURES             Location/Qualifiers
     source          1..10702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    179..296
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        341..1629
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      1639..1648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2462..2480)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2730..2749
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3133..3721
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3186..3206)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3604..3615)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3724..3740)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3725..3741)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4246..4426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4452..4469)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4458..4682
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4728..5156
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4815..4834)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5025..5046
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5165..5185)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5170..5499
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5350..5485
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5412..5431
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5547..5594
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5613..5987
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6117..6238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6154..6173)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6208..6227
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6287..6303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6287..6303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6300..6322)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6311..6327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6335..6341,6342..6359,6360..6365))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6380..6401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6518..6535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6689..7277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6769..6788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7448..8239,8240..8308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8071..8090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8309..8413)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8488..8507)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8679..9058
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9060..9258
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9213..9233
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9276..9456
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9503..9628
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10121..10354
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10539..10583
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
       61 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
      121 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
      181 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
      241 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
      301 tttattacag ggacagcaga gatccagttt ggttaattaa cattatggcc ttaggtcact
      361 tcatctccat ggggttcttc ttctgatttt ctagaaaatg agatgggggt gcagagagct
      421 tcctcagtga cctgcccagg gtcacatcag aaatgtcaga gctagaactt gaactcagat
      481 tactaatctt aaattccatg ccttgggggc atgcaagtac gatatacaga aggagtgaac
      541 tcattagggc agatgaccaa tgagtttagg aaagaagagt ccagggcagg gtacatctac
      601 accacccgcc cagccctggg tgagtccagc cacgttcacc tcattatagt tgcctctctc
      661 cagtcctacc ttgacgggaa gcacaagcag aaactgggac aggagcccca ggagaccaaa
      721 tcttcatggt ccctctggga ggatgggtgg ggagagctgt ggcagaggcc tcaggagggg
      781 ccctgctgct cagtggtgac agataggggt gagaaagcag acagagtcat tccgtcagca
      841 ttctgggtct gtttggtact tcttctcacg ctaaggtggc ggtgtgatat gcacaatggc
      901 taaaaagcag ggagagctgg aaagaaacaa ggacagagac agaggccaag tcaaccagac
      961 caattcccag aggaagcaaa gaaaccatta cagagactac aagggggaag ggaaggagag
     1021 atgaattagc ttcccctgta aaccttagaa cccagctgtt gccagggcaa cggggcaata
     1081 cctgtctctt cagaggagat gaagttgcca gggtaactac atcctgtctt tctcaaggac
     1141 catcccagaa tgtggcaccc actagccgtt accatagcaa ctgcctcttt gccccactta
     1201 atcccatccc gtctgttaaa agggccctat agttggaggt gggggaggta ggaagagcga
     1261 tgatcacttg tggactaagt ttgttcgcat ccccttctcc aaccccctca gtacatcacc
     1321 ctgggggaac agggtccact tgctcctggg cccacacagt cctgcagtat tgtgtatata
     1381 aggccagggc aaagaggagc aggttttaaa gtgaaaggca ggcaggtgtt ggggaggcag
     1441 ttaccggggc aacgggaaca gggcgtttcg gaggtggttg ccatggggac ctggatgctg
     1501 acgaaggctc gcgaggctgt gagcagccac agtgccctgc tcagaagccc caagctcgtc
     1561 agtcaagccg gttctccgtt tgcactcagg agcacgggca ggcgagtggc ccctagttct
     1621 gggggcagcg ggggatccgc caccatggct gacgtggaaa ccgagaccgg catgattgca
     1681 cagtggattg tctttgctat tatggctgct gctgctattg cttttggagt ggctgtgcac
     1741 tttcggcctt cagagctgaa gagcgcatac tatatcaaca ttgccatctg cactatcgcc
     1801 gctaccgctt actatgcaat ggccgtgaac taccaggacc tgacaatgaa tggtgaaagg
     1861 caggtggtct acgcaagata tattaactgg gtgctgacca caccactgct cctgctcgat
     1921 ctcatcgtca tgaccaagat gggcggagtg atgatttctt gggtcatcgg cgcagacatt
     1981 ttcatgatcg tgtttggtat tctgggcgcc ttcgaggatg aacacaagtt caaatgggtg
     2041 tactttatcg ctggatgtgt gatgcaggca gtcctgacat acgggatgta taacgccact
     2101 tggaaagacg atctgaagaa aagccccgag taccatagct cctatgtcag tctgctcgtc
     2161 ttcctgtcaa tcctctgggt gttttatcct gtcgtgtggg ctttcgggtc tggtagtggc
     2221 gtgctgtccg tcgacaatga ggccattctc atgggaatcc tggatgtgct cgctaagcca
     2281 ctgtttggaa tggggtgcct cattgcccat gagactatct tcaagaaaat gctccgaagc
     2341 ggactagcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac
     2401 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     2461 gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct
     2521 cagttcatgt acggctccag ggccttcacc aagcaccccg ccgacatccc cgactactat
     2581 aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     2641 gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta caaggtgaag
     2701 ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac aatgggctgg
     2761 gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga cattaagatg
     2821 gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac ctacaaggcc
     2881 aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc gcaagttgga catcacctcc
     2941 cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgagggccg ccactccacc
     3001 ggcggcatgg acgagctgta caagaagagc aggatcacca gcgagggcga gtacatcccc
     3061 ctggaccaga tcgacatcaa cgtgttctgc tacgagaacg aggtgtaaga attcgatatc
     3121 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3181 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3241 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3301 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     3361 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     3421 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3481 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     3541 ccttggctgc tcgcctgtgt tgccacctgg attctgcggg ggacgtcctt ctgctacgtc
     3601 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     3661 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     3721 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     3781 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     3841 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     3901 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     3961 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     4021 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     4081 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     4141 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     4201 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     4261 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4321 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4381 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     4441 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     4501 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4561 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4621 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4681 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     4741 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4801 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4861 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4921 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4981 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5041 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5101 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     5161 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     5221 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5281 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     5341 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5401 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     5461 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     5521 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     5581 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     5641 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     5701 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     5761 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     5821 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     5881 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     5941 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     6001 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6061 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6121 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6181 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6241 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6301 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6361 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6421 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6481 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6541 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6601 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7741 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8041 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8401 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8461 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8521 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     8581 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     8641 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     8701 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     8761 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     8821 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     8881 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     8941 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     9001 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     9061 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     9121 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     9181 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     9241 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     9301 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9361 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9421 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     9481 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     9541 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     9601 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     9661 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     9721 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     9781 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     9841 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     9901 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     9961 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
    10021 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
    10081 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
    10141 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
    10201 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
    10261 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
    10321 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
    10381 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
    10441 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
    10501 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
    10561 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
    10621 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
    10681 tttctatagt gaatagagtt ag
//
