LOCUS       sequence-253120-       12335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian cell expression of P29S mutant murine RAC1 carrying a 
            modified hormone-binding domain of murine ERalpha at the N-terminus.
            Activation of expressed latent fusion protein induced by 4-OHT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Lionarons DA, Hancock DC, Rana S, East P, Moore C, Murillo MM, 
            Carvalho J, Spencer-Dene B, Herbert E, Stamp G, Damry D, Calado DP, 
            Rosewell I, Fritsch R, Neubig RR, Molina-Arcas M, Downward J
  TITLE     RAC1(P29S) Induces a Mesenchymal Phenotypic Switch via Serum 
            Response Factor to Promote Melanoma Development and Therapy 
            Resistance.
  JOURNAL   Cancer Cell. 2019 Jul 8;36(1):68-83.e9. doi: 
            10.1016/j.ccell.2019.05.015. Epub 2019 Jun 27.
  PUBMED    31257073
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2019.05.015"; journalName: "Cancer Cell"; date: 
            "2019-07-8- 8"; volume: "36"; issue: "1"; pages: "68-83.e9"
COMMENT     Sequence Label: pLZRS-ER-Rac1-P29S-shortlinker
FEATURES             Location/Qualifiers
     source          1..12335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             3490..3959
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4089..4106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4260..4848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4340..4359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4971..5105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4995..5014)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5049..5068
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5335..5934)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5418..5438)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5915..5934
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6017..6516)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6065..6084)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6458..6481
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      8551..10340
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(10834..11625,11626..11694))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11457..11476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11695..11799)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgccg gatccaccat gcgaaatgaa atgggtgctt caggagacat
     1801 gagggctgcc aacctttggc caagccctct tgtgattaag cacactaaga agaatagccc
     1861 tgccttgtcc ttgacagctg accagatggt cagtgccttg ttggatgctg aaccgcccat
     1921 gatctattct gaatatgatc cttctagacc cttcagtgaa gcctcaatga tgggcttatt
     1981 gaccaaccta gcagataggg agctggttca tatgatcaac tgggcaaaga gagtgccagg
     2041 ctttggggac ttgaatctcc atgatcaggt ccaccttctc gagtgtgcct ggctggagat
     2101 tctgatgatt ggtctcgtct ggcgctccat ggaacacccg gggaagctcc tgtttgctcc
     2161 taacttgctc ctggacagga atcaaggtaa atgtgtggaa ggcatggtgg agatctttga
     2221 catgttgctt gctacgtcaa gtcggttccg catgatgaac ctgcagggtg aagagtttgt
     2281 gtgcctcaaa tccatcattt tgcttaattc cggagtgtac acgtttctgt ccagcacctt
     2341 gaagtctctg gaagagaagg accacatcca ccgtgtcctg gacaagatca cagacacttt
     2401 gatccacctg atggccaaag ctggcctgac tctgcagcag cagcatcgcc gcctagctca
     2461 gctccttctc attctttccc atatccggca tatgagtaac aaacgcatgg agcatctcta
     2521 caacatgaaa tgcaagaacg tggtacccct ctatgacctg ctcctggaga tgttggatgc
     2581 ccaccgcctt catgccccag ccagtcgcat gggagtgccc ccagaggagc ccagccagac
     2641 ccagctggcc accaccagct ccacttcagc acattcctta caaacctact acataccccc
     2701 ggaagcagag ggcttcccca acgatcaggc catcaagtgt gtggtggtgg gagacggagc
     2761 tgttggtaaa acctgcctgc tcatcagtta cacgaccaat gcatttagtg gagagtacat
     2821 ccccaccgtc tttgacaact attctgccaa tgttatggta gatggaaaac cagtgaatct
     2881 gggcctatgg gacacagctg gacaagaaga ttatgacaga ttgcgtcccc tctcctaccc
     2941 gcagacagac gtgttcttaa tttgcttttc ccttgtgagt cctgcatcat ttgaaaatgt
     3001 ccgtgcaaag tggtatcctg aagtgcgaca ccactgtccc aatactccta tcatcctcgt
     3061 ggggacgaag cttgatctta gggatgataa ggacaccatt gagaagctga aggagaagaa
     3121 gctgactccc atcacctacc cgcaggggct ggccatggcg aaagagatcg gtgctgtcaa
     3181 atacctggag tgctcagctc tcacacagcg aggactcaag acagtgtttg acgaagctat
     3241 ccgagcggtt ctctgtcccc ctcctgtcaa gaagaggaag agaaaatgcc tgctgttgta
     3301 agcggccgcc agcacagtgg tcgacgataa aataaaagat tttatttagt ctccagaaaa
     3361 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     3421 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     3481 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     3541 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     3601 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     3661 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     3721 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     3781 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     3841 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     3901 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
     3961 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     4021 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     4081 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4141 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4201 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4261 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4321 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4381 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4441 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4501 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4561 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4621 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4681 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4741 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4801 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4861 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4921 atgagattat caaaaaggat cttcacctag atccttttgc ggccgaatta gatccagaca
     4981 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5041 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5101 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     5161 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagagt
     5221 cggtgggcct cgggggcggg tgcggggtcg gcggggccgc cccgggtggc ttcggtcgga
     5281 gccatggggt cgtgcgctcc tttcggtcgg gcgctgcggg tcgtggggcg ggcgtcaggc
     5341 accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga cgtcggcggt
     5401 gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa
     5461 ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag
     5521 acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg cgggctcctt
     5581 gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct
     5641 caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg
     5701 cgtggtccag accgccaccg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag
     5761 cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg
     5821 gtcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc
     5881 ccgggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcatggaagg
     5941 tcgtctcctt gtgaggggtc aggggcgtgg gtcaggggat ggtggcggca ccggtcgtgg
     6001 cggccgacct gcaggtcgaa aggcccggag atgaggaaga ggagaacagc gcggcagacg
     6061 tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg cccgccccgc
     6121 ccctgagccc gcccctgagc ccgcccccgg acccacccct tcccagcctc tgagcccaga
     6181 aagcgaagga gcaaagctgc tattggccgc tgccccaaag gcctacccgc ttccattgct
     6241 cagcggtgct gtccatctgc acgagactag tgagacgtgc tacttccatt tgtcacgtcc
     6301 tgcacgacgc gagctgcggg gcggggggga acttcctgac taggggagga gtagaaggtg
     6361 gcgcgaaggg gccaccaaag aacggagccg gttggcgcct accggtggat gtggaatgtg
     6421 tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag cggggctgct aaagcgcatg
     6481 ctccagactg ccttgggaaa agcgcctccc ctacccggta gaattaattc tcatgtttga
     6541 cagcttatca tcgataagct gatcctcaca ggccgcaccc agcttttctt ccgttgcccc
     6601 agtagcatct ctgtctggtg accttgaaga ggaagaggag gggtcccgag aatccccatc
     6661 cctaccgtcc agcaaaaagg gggacgagga atttgaggcc tggcttgagg ctcaggacgc
     6721 aaatcttgag gatgttcagc gggagttttc cgggctgcga gtaattggtg atgaggacga
     6781 ggatggttcg gaggatgggg aattttcaga cctggatctg tctgacagcg accatgaagg
     6841 ggatgagggt gggggggctg ttggaggggg caggagtctg cactccctgt attcactgag
     6901 cgtcgtctaa taaagatgtc tattgatctc ttttagtgtg aatcatgtct gacgaggggc
     6961 caggtacagg acctggaaat ggcctaggag agaagggaga cacatctgga ccagaaggct
     7021 ccggcggcag tggacctcaa agaagagggg gtgataacca tggacgagga cggggaagag
     7081 gacgaggacg aggaggcgga agaccaggag ccccgggcgg ctcaggatca gggccaagac
     7141 atagagatgg tgtccggaga ccccaaaaac gtccaagttg cattggctgc aaagggaccc
     7201 acggtggaac aggagcagga gcaggagcgg gaggggcagg agcaggaggg gcaggagcag
     7261 gaggaggggc aggagcagga ggnnnggcag gagcaggagg tggaggccgg ggtcgaggag
     7321 gcagtggagg ccggggtcga ggaggtagtg gaggccgggg tcgaggaggt agtggaggcc
     7381 gccggggtag aggacgtgaa agagccaggg ggggaagtcg tgaaagagcc agggggagag
     7441 gtcgtggacg tggagaaaag aggcccagga gtcccagtag tcagtcatca tcatccgggt
     7501 ctccaccgcg caggccccct ccaggtagaa ggccattttt ccaccctgta ggggaagccg
     7561 attattttga ataccaccaa gaaggtggcc cagatggtga gcctgacgtg cccccgggag
     7621 cgatagagca gggccccgca gatgacccag gagaaggccc aagcactgga ccccggggtc
     7681 agggtgatgg aggcaggcgc aaaaaaggag ggtggtttgg aaagcatcgt ggtcaaggag
     7741 gttccaaccc gaaatttgag aacattgcag aaggtttaag agctctcctg gctaggagtc
     7801 acgtagaaag gactaccgac gaaggaactt gggtcgccgg tgtgttcgta tatggaggta
     7861 gtaagacctc cctttacaac ctaaggcgag gaactgccct tgctattcca caatgtcgtc
     7921 ttacaccatt gagtcgtctc ccctttggaa tggcccctgg acccggccca caacctggcc
     7981 cgctaaggga gtccattgtc tgttatttca tggtcttttt acaaactcat atatttgctg
     8041 aggttttgaa ggatgcgatt aaggaccttg ttatgacaaa gcccgctcct acctgcaata
     8101 tcagggtgac tgtgtgcagc tttgacgatg gagtagattt gcctccctgg tttccaccta
     8161 tggtggaagg ggctgccgcg gagggtgatg acggagatga cggagatgaa ggaggtgatg
     8221 gagatgaggg tgaggaaggg caggagtgat gtaacttgtt aggagacgcc ctcaatcgta
     8281 ttaaaagccg tgtattcccc cgcactaaag aataaatccc cagtagacat catgcgtgct
     8341 gttggtgtat ttctggccat ctgtcttgtc accattttcg tcctcccaac atggggcaat
     8401 tgggcatacc catgttgtca cgtcactcag ctccgcgctc aacaccttct cgcgttggaa
     8461 aacattagcg acatttacct ggtgagcaat cagacatgcg acggctttag cctggcctcc
     8521 ttaaattcac ctaagaatgg gagcaaccag caggaaaagg acaagcagcg aaaattcacg
     8581 cccccttggg aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc
     8641 atatgctgac tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc
     8701 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
     8761 ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc
     8821 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc
     8881 atatgctacc cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat
     8941 gctacccaga tataaattag gatagcatat actaccctaa tctctattag gatagcatat
     9001 gctacccgga tacagattag gatagcatat actacccaga tatagattag gatagcatat
     9061 gctacccaga tatagattag gatagcctat gctacccaga tataaattag gatagcatat
     9121 actacccaga tatagattag gatagcatat gctacccaga tatagattag gatagcctat
     9181 gctacccaga tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta
     9241 cccatggcaa cattagccca ccgtgctctc agcgacctcg tgaatatgag gaccaacaac
     9301 cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc
     9361 gcccctatct tggcccgccc acctacttat gcaggtattc cccggggtgc cattagtggt
     9421 tttgtgggca agtggtttga ccgcagtggt tagcggggtt acaatcagcc aagttattac
     9481 acccttattt tacagtccaa aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac
     9541 tccacaattt caaaaaaaag agtggccact tgtctttgtt tatgggcccc attggcgtgg
     9601 agccccgttt aattttcggg ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt
     9661 ccactctctt tccccttgtt acaaatagag tgtaacaaca tggttcacct gtcttggtcc
     9721 ctgcctggga cacatcttaa taaccccagt atcatattgc actaggatta tgtgttgccc
     9781 atagccataa attcgtgtga gatggacatc cagtctttac ggcttgtccc caccccatgg
     9841 atttctattg ttaaagatat tcagaatgtt tcattcctac actagtattt attgcccaag
     9901 gggtttgtga gggttatatt ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa
     9961 attttattct gggggcgtca cctgaaacct tgttttcgag cacctcacat acaccttact
    10021 gttcacaact cagcagttat tctattagct aaacgaagga gaatgaagaa gcaggcgaag
    10081 attcaggaga gttcactgcc cgctccttga tcttcagcca ctgcccttgt gactaaaatg
    10141 gttcactacc ctcgtggaat cctgacccca tgtaaataaa accgtgacag ctcatggggt
    10201 gggagatatc gctgttcctt aggacccttt tactaaccct aattcgatag catatgcttc
    10261 ccgttgggta acatatgcta ttgaattagg gttagtctgg atagtatata ctactacccg
    10321 ggaagcatat gctacccgtt tagggttaac aagggggcct tataaacact attgctaatg
    10381 ccctcttgag ggtccgctta tcggtagcta cacaggcccc tctgattgac gttggtgtag
    10441 cctcccgtag tcttcctggg cccctgggag gtacatgtcc cccagcattg gtgtaagagc
    10501 ttcagccaag agttacacat aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct
    10561 gctccaggat gaaagccact cagtgttggc aaatgtgcac atccatttat aaggatgtca
    10621 actacagtca gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc
    10681 ccagttggca agttgtacca accaactgaa gggattacat gcactgcccc gcgaagaagg
    10741 ggcagagatg tcgtagtcag gtttagttcg tccggggcgg ggatctggcc gcaaatcaat
    10801 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10861 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10921 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10981 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    11041 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    11101 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    11161 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    11221 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    11281 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    11341 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    11401 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11461 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11521 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11581 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11641 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11701 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11761 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11821 accagctttg ctcttaggag tttcctaata catcccaaac tcaaatatat aaagcatttg
    11881 acttgttcta tgccctaggg ggcgggggga agctaagcca gcttttttta acatttaaaa
    11941 tgttaattcc attttaaatg cacagatgtt tttatttcat aagggtttca atgtgcatga
    12001 atgctgcaat attcctgtta ccaaagctag tataaataaa aatagataaa cgtggaaatt
    12061 acttagagtt tctgtcatta acgtttcctt cctcagttga caacataaat gcgctgctga
    12121 gaagccagtt tgcatctgtc aggatcaatt tcccattatg ccagtcatat taattactag
    12181 tcaattagtt gatttttatt tttgacatat acatgtgaaa gaccccacct gtaggtttgg
    12241 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
    12301 aaagttcaga tcaaggtcag gaacagatgg aacag
//
