LOCUS       sequence-253146-        3249 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC12A7
FEATURES             Location/Qualifiers
     source          1..3249
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcctacca acttcacagt ggtgccagtg gaggcacacg cagacggagg aggcgatgag
       61 accgcagaga ggacagaggc accaggaacc ccagagggac cagagcccga gcgccctagc
      121 ccaggcgacg gcaacccacg ggagaattcc cccttcctga acaatgtgga ggtggagcag
      181 gagtccttct ttgagggcaa gaatatggcc ctgtttgagg aggagatgga ttctaaccct
      241 atggtgagct ccctgctgaa taagctggcc aactatacca atctgagcca gggagtggtg
      301 gagcacgagg aggacgagga gtcccggaga agggaggcaa aggcaccaag aatgggcaca
      361 ttcatcggcg tgtacctgcc ctgcctgcag aacatcctgg gcgtgatcct gttcctgagg
      421 ctgacctgga tcgtgggagt ggcaggcgtg ctggagtctt ttctgatcgt ggccatgtgc
      481 tgtacctgta caatgctgac agccatctct atgagcgcca tcgcaaccaa tggagtggtg
      541 ccagcaggag gcagctacta tatgatctcc cgctctctgg gacctgagtt tggaggagca
      601 gtgggactgt gcttctacct gggcaccaca tttgccggcg ccatgtatat cctgggcacc
      661 atcgagatct tcctgacata tatcagccca ggcgccgcca tctttcaggc agaggcagca
      721 ggaggagagg cagcagccat gctgcacaac atgcgggtgt acggcacctg cacactggtg
      781 ctgatggccc tggtggtgtt cgtgggcgtg aagtatgtga ataagctggc cctggtgttt
      841 ctggcctgcg tggtgctgtc tatcctggcc atctacgccg gcgtgatcaa gagcgccttc
      901 gacccccctg atatccccgt gtgcctgctg ggcaacagaa cactgtctcg ccggagcttt
      961 gatgcttgcg ttaaagccta tggcattcac aacaattccg ccacctctgc cctgtggggc
     1021 ctgttctgca atggaagcca gccatccgcc gcatgtgacg agtacttcat ccagaacaat
     1081 gtgacagaga tccagggaat ccctggagca gcaagcggcg tgttcctgga gaacctgtgg
     1141 tccacctacg cacacgcagg agcctttgtg gagaagaagg gagtgccttc cgtgccagtg
     1201 gcagaggagt ctagagcaag cgccctgcct tacgtgctga cagatatcgc cgcctccttc
     1261 accctgctgg tgggcatcta ttttccatct gtgaccggca tcatggccgg ctccaacagg
     1321 tctggcgacc tgaaggatgc ccagaagagc atccccaccg gcacaatcct ggccatcgtg
     1381 accacatctt tcatctacct gagctgcatc gtgctgtttg gcgcctgtat cgagggcgtg
     1441 gtgctgaggg acaagttcgg cgaggccctg cagggcaacc tggtcatcgg aatgctggca
     1501 tggccaagcc cttgggtcat cgtgatcggc agcttctttt ccacatgcgg agcaggactg
     1561 cagtccctga ccggagcacc tagactgctg caggcaatcg ccagggatgg catcgtgcca
     1621 ttcctgcagg tgtttggaca cggcaaggca aacggagagc caacctgggc actgctgctg
     1681 acagtgctga tctgtgagac cggcatcctg atcgcctctc tggacagcgt ggcccccatc
     1741 ctgagcatgt tctttctgat gtgctacctg ttcgtgaacc tggcatgtgc agtgcagacc
     1801 ctgctgagga cacccaattg gcgccctcgg ttcaagtttt atcactggac cctgtccttc
     1861 ctgggcatgt ctctgtgcct ggccctgatg tttatctgtt cttggtacta tgcactgagc
     1921 gccatgctga tcgcaggatg catctacaag tatatcgagt acagaggcgc cgagaaggag
     1981 tggggcgatg gcatcagggg cctgtccctg aatgcagcaa gatatgcact gctgagggtg
     2041 gagcacggac caccacacac aaagaactgg agaccacagg tgctggtcat gctgaatctg
     2101 gacgcagagc aggcagtgaa gcacccaagg ctgctgtcct tcacctctca gctgaaggcc
     2161 ggcaagggcc tgacaatcgt gggcagcgtg ctggagggca cctatctgga taagcacatg
     2221 gaggcccaga gagccgagga gaacatcagg agcctgatgt ccaccgagaa gacaaagggc
     2281 ttttgccagc tggtggtgtc tagctccctg cgggacggca tgagccacct gatccagtcc
     2341 gccggactgg gaggcctgaa gcacaataca gtgctgatgg cctggcccgc ctcttggaag
     2401 caggaggata accctttcag ctggaagaat tttgtggaca ccgtgcgcga taccacagca
     2461 gcacaccagg ccctgctggt ggccaagaac gtggactcct tccctcagaa tcaggagcgg
     2521 tttggcggcg gccacatcga cgtgtggtgg atcgtgcacg atggcggcat gctgatgctg
     2581 ctgccattcc tgctgcgcca gcacaaagtg tggcggaagt gtagaatgag gatctttacc
     2641 gtggcccagg tggacgataa cagcatccag atgaagaagg atctgcagat gttcctgtac
     2701 cacctgagaa tctctgccga agtggaggtg gtggagatgg tggagaatga catcagcgcc
     2761 tttacctatg agcgcacact gatgatggag cagcggtccc agatgctgaa gcagatgcag
     2821 ctgtctaaga acgagcagga gagagaggcc cagctgatcc acgacaggaa taccgcctcc
     2881 cacacagcag cagcagcaag gacccaggca cctccaacac ccgataaggt gcagatgacc
     2941 tggacacggg agaagctgat cgccgagaag taccgctctc gggacacaag cctgtccggc
     3001 ttcaaggacc tgttttccat gaagcccgat cagtctaacg tgagaaggat gcacaccgcc
     3061 gtgaagctga acggcgtggt gctgaataag tcccaggacg cccagctggt gctgctgaac
     3121 atgcctggac cacctaagaa caggcagggc gatgagaatt atatggagtt cctggaggtg
     3181 ctgacagagg gcctgaatcg ggtgctgctg gtgagaggag gaggaagaga agtgatcacc
     3241 atctacagc
//
