LOCUS       sequence-253192-        3297 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC12A1
FEATURES             Location/Qualifiers
     source          1..3297
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagcctga acaatagctc caacgtgttc ctggattccg tgccctctaa caccaataga
       61 tttcaggtgt ccgtgatcaa cgagaatcac gagtctagcg ccgccgccga cgataataca
      121 gatccccctc actacgagga gacctccttc ggcgacgagg cacagaagcg gctgagaatc
      181 tctttcaggc ccggcaacca ggagtgctat gataattttc tgcagagcgg cgagaccgcc
      241 aagacagatg cctctttcca cgcctacgac agccacacca acacatacta tctgcagaca
      301 tttggccaca ataccatgga cgccgtgcct aagatcgagt actatcgcaa caccggctcc
      361 atctctggcc caaaagtgaa tcggccctcc ctgctggaga tccacgagca gctggccaag
      421 aacgtggcag tgaccccatc ctctgccgac agggtggcaa acggcgatgg aatccctggc
      481 gacgagcagg ccgagaataa ggaggacgat caggccggcg tggtgaagtt cggctgggtg
      541 aagggcgtgc tggtgagatg catgctgaac atctggggcg tgatgctgtt tatcaggctg
      601 agctggatcg tgggagaggc aggaatcgga ctgggcgtgc tgatcatcct gctgtccacc
      661 atggtgacat ctatcaccgg cctgagcaca tccgccatcg ccaccaatgg cttcgtgaga
      721 ggcggcggcg cctactatct gatctctagg agcctgggcc ctgagtttgg cggctccatc
      781 ggcctgatct tcgcctttgc caacgcagtg gcagtggcaa tgtacgtggt gggcttcgcc
      841 gagaccgtgg tggatctgct gaaggagtcc gactctatga tggtggatcc aacaaatgac
      901 atccggatca tcggctctat caccgtggtc atcctgctgg gcatcagcgt ggcaggaatg
      961 gagtgggagg caaaggccca ggtcatcctg ctggtcatcc tgctgatcgc catcgccaac
     1021 ttctttatcg gcaccgtgat cccttccaac aatgagaaga agtctagagg cttctttaac
     1081 tatcaggcct ctatcttcgc cgagaatttt ggccccaggt tcaccaaggg cgagggcttc
     1141 tttagcgtgt tcgccatctt ctttcctgca gcaacaggaa tcctggcagg agcaaatatc
     1201 agcggcgacc tggaggatcc tcaggacgca atcccaaggg gaaccatgct ggccatcttt
     1261 atcaccacag tggcctacct gggagtggca atctgcgtgg gagcatgcgt ggtgcgggat
     1321 gccacaggca acatgaatga caccatcatc tccggcatga actgcaatgg ctctgccgcc
     1381 tgtggcctgg gctatgattt cagcaggtgc cgccacgagc catgtcagta cggcctgatg
     1441 aacaattttc aggtcatgag catggtgtcc ggattcggac cactgatcac agcaggcatc
     1501 ttttccgcca ccctgagctc cgccctggca tctctggtga gcgccccaaa ggtgtttcag
     1561 gccctgtgca aggacaacat ctacaaggcc ctgcagttct ttgccaaggg ctatggcaag
     1621 aacaatgagc ctctgagagg ctacatcctg acctttctga tcgccatggc cttcatcctg
     1681 atcgccgagc tgaacacaat cgccccaatc atcagcaatt tctttctggc ctcctatgcc
     1741 ctgatcaatt tctcctgttt tcacgcctct tacgccaaga gccctggctg gaggccagcc
     1801 tacggcatct ataacatgtg ggtgtctctg ttcggcgccg tgctgtgctg tgccgtgatg
     1861 tttgtgatca attggtgggc cgccgtgatc acatatgtga tcgagttctt tctgtacgtg
     1921 tatgtgacct gcaagaagcc cgatgtgaac tggggctcta gcacacaggc cctgtcctac
     1981 gtgtctgccc tggataacgc cctggagctg accacagtgg aggaccacgt gaagaatttc
     2041 cgccctcagt gtatcgtgct gacaggagga ccaatgacca ggcctgccct gctggacatc
     2101 acacacgcct tcaccaagaa ttctggcctg tgcatctgct gtgaggtgtt tgtgggccct
     2161 agaaagctgt gcgtgaagga gatgaacagc ggcatggcca agaagcaggc ctggctgatc
     2221 aagaataaga tcaaggcctt ctacgcagca gtggcagcag attgctttag ggacggcgtg
     2281 cgcagcctgc tgcaggcatc cggcctgggc cgcatgaagc ctaacaccct ggtcatcggc
     2341 tataagaaga attggcggaa ggccccactg acagagatcg agaactacgt gggcatcatc
     2401 cacgatgcct tcgactttga gatcggcgtg gtcatcgtgc ggatctctca gggcttcgat
     2461 atcagccagg tgctgcaggt gcaggaggag ctggagcggc tggagcagga gagactggcc
     2521 ctggaggcca ccatcaagga caacgagtgt gaggaggaga gcggaggaat caggggactg
     2581 tttaagaagg ccggcaagct gaatatcacc aagaccacac caaagaagga cggctccatc
     2641 aacacaagcc agtccatgca cgtgggcgag ttcaatcaga agctggtgga ggcctctaca
     2701 cagttcaaga agaagcagga gaagggcacc atcgacgtgt ggtggctgtt cgacgatggc
     2761 ggcctgacac tgctgatccc ctatatcctg accctgagga agaagtggaa ggactgcaag
     2821 ctgcgcatct acgtgggcgg caagatcaac cggatcgagg aggagaagat cgtgatggcc
     2881 agcctgctgt ccaagtttag aatcaagttc gccgatatcc acatcatcgg cgacatcaac
     2941 atcaggccaa ataaggagag ctggaaggtg tttgaggaga tgatcgagcc ctatcgcctg
     3001 cacgagtcct gtaaggatct gaccacagcc gagaagctga agcgggagac accatggaag
     3061 atcaccgacg ccgagctgga ggccgtgaag gagaagagct accggcaggt gagactgaac
     3121 gagctgctgc aggagcacag cagagccgcc aatctgatcg tgctgtccct gccagtggca
     3181 aggaagggat ctatcagcga cctgctgtat atggcctggc tggagatcct gaccaagaac
     3241 ctgccacccg tgctgctggt gcggggcaac cacaagaatg tgctgacatt ctactcc
//
