LOCUS       sequence-253206-        1653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC22A4
FEATURES             Location/Qualifiers
     source          1..1653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      523..532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgcgggact acgatgaagt gatcgccttt ctgggcgagt ggggcccttt ccagagactg
       61 atcttctttc tgctgtctgc cagcatcatc ccaaacggct ttaatggcat gtctgtagta
      121 tttcttgcag gaacccctga gcacaggtgc agagtgccag acgcagcaaa cctgagctcc
      181 gcctggagga acaatagcgt gccactgagg ctgcgcgatg gcagagaggt gccccactcc
      241 tgttctcggt atagactggc cacaatcgcc aatttttccg ccctgggact ggagccagga
      301 agggacgtgg atctgggaca gctggagcag gagtcttgcc tggacggctg ggagttctct
      361 caggacgtgt acctgagcac cgtggtgaca gagtggaacc tggtgtgcga ggacaattgg
      421 aaggtgcccc tgaccacatc tctgttcttt gtgggcgtgc tgctgggctc ttttgtgagc
      481 ggccagctga gcgatcggtt tggcagaaag aacgtgctgt tcgccaccat ggccgtgcag
      541 acaggcttca gctttctgca gatcttcagc atctcctggg agatgttcac cgtgctgttt
      601 gtgatcgtgg gcatgggcca gatctccaat tatgtggtgg cctttatcct gggcacagag
      661 atcctgggca agtccgtgag gatcatcttc tctaccctgg gcgtgtgcac attctttgcc
      721 gtgggctaca tgctgctgcc tctgttcgcc tacttcatca gggactggag gatgctgctg
      781 ctggccctga ccgtgccagg cgtgctgtgc gtgccactgt ggtggtttat ccccgagagc
      841 cctaggtggc tgatctccca gcggagattc cgcgaggccg aggatatcat ccagaaggcc
      901 gccaagatga acaatatcgc cgtgcctgcc gtgatcttcg actccgtgga ggagctgaac
      961 ccactgaagc agcagaaggc cttcatcctg gatctgttta ggacccgcaa tatcgccatc
     1021 atgacaatca tgtctctgct gctgtggatg ctgacctctg tgggctactt tgccctgagc
     1081 ctggacgccc caaacctgca cggcgatgcc tacctgaatt gcttcctgag cgccctgatc
     1141 gagatccccg cctatatcac cgcctggctg ctgctgagga cactgcctag gcgctacatc
     1201 atcgcagccg tgctgttttg gggaggaggc gtgctgctgt tcatccagct ggtgcccgtg
     1261 gactactatt ttctgagcat cggcctggtc atgctgggca agttcggcat cacatccgcc
     1321 ttttctatgc tgtacgtgtt caccgccgag ctgtatccta cactggtgag gaacatggca
     1381 gtgggagtga ccagcacagc atccagggtg ggatccatca tcgcaccata cttcgtgtat
     1441 ctgggcgcct acaatcgcat gctgccctat atcgtgatgg gcagcctgac cgtgctgatc
     1501 ggcatcctga cactgttctt tcccgagtcc ctgggcatga ccctgcctga gacactggag
     1561 cagatgcaga aggtgaagtg gtttcggtcc ggcaagaaga ccagagattc tatggagaca
     1621 gaggagaacc ccaaggtgct gatcaccgcc ttc
//
