LOCUS       sequence-253216-        3636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC12A2
FEATURES             Location/Qualifiers
     source          1..3636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggagccac ggcccacagc cccaagctcc ggagcaccag gactggcagg agtgggagag
       61 accccatccg ccgccgccct ggccgccgcc agagtggagc tgcccggcac agccgtgccc
      121 agcgtgcctg aggacgccgc ccctgcctcc agggacggcg gcggcgtgcg cgatgagggc
      181 cctgccgccg ccggcgacgg cctgggcagg ccactgggac ctaccccaag ccagtcccgc
      241 ttccaggtgg atctggtgtc cgagaacgcc ggcagagccg ccgcagccgc cgcagcagca
      301 gcagcagccg ccgccgccgc tggagccggc gccggcgcca agcagacccc cgccgacggc
      361 gaggcatctg gagagagcga gcctgcaaag ggaagcgagg aggcaaaggg ccggttcaga
      421 gtgaattttg tggatcctgc cgcctctagc tccgccgagg actctctgag cgatgcagca
      481 ggagtgggag tggacggacc aaacgtgtcc ttccagaatg gcggcgatac agtgctgtct
      541 gagggctcta gcctgcactc tggcggagga ggaggaagcg gacaccacca gcactactat
      601 tacgacaccc acacaaacac ctattacctg cggacatttg gccacaatac catggacgcc
      661 gtgcctcgga tcgatcacta cagacacacc gcagcacagc tgggagagaa gctgctgaga
      721 ccaagcctgg ccgagctgca cgacgagctg gagaaggagc ccttcgagga tggctttgcc
      781 aacggcgagg agtccacacc taccagggat gccgtggtga catataccgc cgagtctaag
      841 ggcgtggtga agttcggctg gatcaagggc gtgctggtgc ggtgcatgct gaatatctgg
      901 ggcgtgatgc tgtttatcag actgagctgg atcgtgggac aggcaggaat cggactgagc
      961 gtgctggtca tcatgatggc cacagtggtg accacaatca caggcctgtc cacctctgcc
     1021 atcgcaacca acggattcgt gcggggagga ggcgcctatt acctgatcag cagatccctg
     1081 ggaccagagt ttggaggagc aatcggcctg atcttcgcct ttgccaacgc agtggcagtg
     1141 gcaatgtacg tggtgggctt cgccgagaca gtggtggagc tgctgaagga gcactccatc
     1201 ctgatgatcg acgagatcaa tgatatcagg atcatcggcg ccatcaccgt ggtcatcctg
     1261 ctgggcatca gcgtggcagg aatggagtgg gaggcaaagg cccagatcgt gctgctggtc
     1321 atcctgctgc tggccatcgg cgactttgtg atcggcacct tcatcccact ggagtctaag
     1381 aagcccaagg gcttctttgg ctacaagagc gagatcttca acgagaattt tggccccgat
     1441 ttccgcgagg aggagacatt ctttagcgtg ttcgccatct tctttcctgc agcaaccgga
     1501 atcctggcag gagcaaacat ctccggcgac ctggccgatc ctcagtctgc catcccaaag
     1561 ggcacactgc tggccatcct gatcaccaca ctggtgtacg tgggcatcgc cgtgtccgtg
     1621 ggatcttgcg tggtgcggga cgcaaccgga aacgtgaatg atacaatcgt gaccgagctg
     1681 acaaactgca ccagcgccgc ctgtaagctg aatttcgact tttcctcttg cgagagctcc
     1741 ccatgttcct atggcctgat gaacaatttt caggtcatga gcatggtgtc cggcttcaca
     1801 cccctgatca gcgccggcat cttttccgcc accctgtcta gcgccctggc atctctggtg
     1861 agcgccccaa agatcttcca ggccctgtgc aaggataaca tctatcccgc cttccagatg
     1921 tttgccaagg gctacggcaa gaacaatgag ccactgagag gctatatcct gacctttctg
     1981 atcgccctgg gcttcatcct gatcgccgag ctgaacgtga tcgcccccat catctccaat
     2041 ttctttctgg cctcttacgc cctgatcaat ttctccgtgt ttcacgcaag cctggcaaag
     2101 tcccctggat ggaggccagc cttcaagtat tacaacatgt ggatcagcct gctgggcgcc
     2161 atcctgtgct gtatcgtgat gtttgtgatc aattggtggg ccgccctgct gacatacgtg
     2221 atcgtgctgg gcctgtatat ctacgtgacc tataagaagc ctgacgtgaa ctggggctcc
     2281 tctacacagg ccctgaccta tctgaatgcc ctgcagcact ctatcaggct gagcggcgtg
     2341 gaggatcacg tgaagaactt ccgcccacag tgcctggtca tgacaggagc accaaattcc
     2401 aggcctgccc tgctgcacct ggtgcacgac tttaccaaga acgtgggcct gatgatctgt
     2461 ggacacgtgc acatgggacc acggagacag gccatgaagg agatgagcat cgatcaggcc
     2521 aagtaccagc ggtggctgat caagaataag atgaaggcct tctatgcacc agtgcacgca
     2581 gacgatctga gggagggcgc ccagtacctg atgcaggcag caggactggg aaggatgaag
     2641 cctaacaccc tggtgctggg ctttaagaag gactggctgc aggccgatat gagggacgtg
     2701 gatatgtaca tcaatctgtt tcacgacgcc ttcgatatcc agtatggcgt ggtggtcatc
     2761 cgcctgaagg agggcctgga catcagccac ctgcagggcc aggaggagct gctgagctcc
     2821 caggagaagt ctccaggcac caaggatgtg gtggtgagcg tggagtactc caagaagtct
     2881 gacctggata catccaagcc cctgtctgag aagcctatca cccacaaggt ggaggaggag
     2941 gacggcaaga cagccaccca gcccctgctg aagaaggagt ctaagggccc aatcgtgccc
     3001 ctgaacgtgg ccgaccagaa gctgctggag gccagcacac agttccagaa gaagcagggc
     3061 aagaatacca tcgacgtgtg gtggctgttt gacgatggcg gcctgaccct gctgatcccc
     3121 tacctgctga ccaccaagaa gaagtggaag gactgtaaga tccgggtgtt catcggcggc
     3181 aagatcaaca gaatcgacca cgataggcgc gccatggcca cactgctgtc taagtttaga
     3241 atcgacttca gcgatatcat ggtgctgggc gatatcaaca ccaagcctaa gaaggagaat
     3301 atcatcgcct ttgaggagat catcgagcca tatcggctgc acgaggacga taaggagcag
     3361 gacatcgccg ataagatgaa ggaggacgag ccctggagaa tcacagataa cgagctggag
     3421 ctgtataaga caaagaccta caggcagatc cgcctgaacg aactgctgaa ggaacactct
     3481 agcaccgcca atatcatcgt gatgtccctg cctgtggcaa ggaagggagc cgtgtcctct
     3541 gccctgtaca tggcctggct ggaggccctg tctaaggacc tgccacctat cctgctggtg
     3601 cgcggcaatc accagagcgt gctgaccttc tattcc
//
