LOCUS       sequence-253218-        1785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC13A1
FEATURES             Location/Qualifiers
     source          1..1785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      430..439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgaagttct ttagctacat cctggtgtat cggagattcc tgtttgtggt gtttacagtg
       61 ctggtgctgc tgccactgcc catcgtgctg cacacaaagg aggccgagtg cgcctacacc
      121 ctgtttgtgg tggccacatt ctggctgacc gaggccctgc cactgtctgt gaccgccctg
      181 ctgccaagcc tgatgctgcc tatgttcggc atcatgcctt ccaagaaggt ggcctctgcc
      241 tactttaagg acttccacct gctgctgatc ggcgtgatct gtctggccac ctccatcgag
      301 aagtggaacc tgcacaagag aatcgccctg aagatggtca tgatggtggg cgtgaatcct
      361 gcctggctga cactgggctt tatgagctcc accgccttcc tgtctatgtg gctgtctaac
      421 accagcacag ccgccatggt catgccaatc gccgaggccg tggtgcagca gatcatcaat
      481 gccgaggccg aggtggaggc cacacagatg acctacttca acggctccac caatcacggc
      541 ctggagatcg acgagtctgt gaacggccac gagatcaatg agcggaagga gaagacaaag
      601 cctgtgccag gctataacaa tgataccggc aagatctcta gcaaggtgga gctggagaag
      661 aacagcggca tgaggacaaa gtaccgcacc aagaagggcc acgtgacacg gaagctgacc
      721 tgcctgtgca tcgcctattc ctctaccatc ggcggcctga ccacaatcac cggcacatcc
      781 accaacctga tctttgccga gtacttcaat acacggtatc ctgactgcag atgtctgaac
      841 tttggcagct ggttcacctt ttccttccca gccgccctga tcatcctgct gctgtcctgg
      901 atctggctgc agtggctgtt tctgggcttt aatttcaagg agatgttcaa gtgcggcaag
      961 acaaagaccg tgcagcagaa ggcctgtgcc gaagtgatca agcaggagta ccagaagctg
     1021 ggccccatca ggtatcagga gatcgtgacc ctggtgctgt tcatcatcat ggccctgctg
     1081 tggttttcta gggacccagg attcgtgcct ggatggagcg ccctgttttc cgagtaccct
     1141 ggcttcgcca cagatagcac cgtggccctg ctgatcggcc tgctgttctt tctgatcccc
     1201 gccaagacac tgaccaagac cacacctaca ggcgagatcg tggcctttga ttatagccca
     1261 ctgatcacct ggaaggagtt tcagtccttc atgccatggg acatcgcaat cctggtggga
     1321 ggaggattcg cactggcaga tggatgcgag gagtccggac tgtctaagtg gatcggcaat
     1381 aagctgtctc cactgggaag cctgccagca tggctgatca tcctgatcag ctccctgatg
     1441 gtgacatccc tgaccgaggt ggcctctaac cccgccacaa tcaccctgtt cctgccaatc
     1501 ctgagcccac tggcagaggc aatccacgtg aatcctctgt acatcctgat cccatctaca
     1561 ctgtgcacca gctttgcctt cctgctgcca gtggcaaacc cacctaatgc catcgtgttt
     1621 tcctatggcc acctgaaagt gatcgacatg gtgaaggcag gactgggagt gaacatcgtg
     1681 ggagtggccg tggtcatgct gggcatctgt acatggatcg tgccaatgtt cgatctgtac
     1741 acctatccta gctgggcccc agccatgtcc aatgagacca tgccc
//
