LOCUS       sequence-253224-        1260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC17A3
FEATURES             Location/Qualifiers
     source          1..1260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      185..194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggccacca agacagagct gtcccctacc gcccgggagt ctaagaacgc ccaggacatg
       61 caggtggatg agacactgat ccctaggaag gtgccatctc tgtgcagcgc cagatacgga
      121 atcgccctgg tgctgcactt ctgtaacttc accacaatcg cccagaacgt gatcatgaat
      181 atcaccatgg tggccatggt gaactccaca tctccacaga gccagctgaa tgacagctcc
      241 gaggtgctgc ccgtggatag cttcggcggc ctgtccaagg cccccaagtc tctgcctgcc
      301 aagtctagca tcctgggcgg ccagtttgcc atctgggaga agtggggacc acctcaggag
      361 cggtccagac tgtgctctat cgccctgagc ggcatgctgc tgggctgttt caccgccatc
      421 ctgatcggcg gctttatcag cgagacactg ggctggccat tcgtgtttta catcttcgga
      481 ggagtgggat gcgtgtgctg tctgctgtgg tttgtggtca tctacgacga tccagtgtcc
      541 tatccctgga tcagcacctc cgagaaggag tatatcatct cctctctgaa gcagcaagtg
      601 ggcagctcca agcagccact gcccatcaag gccatgctga ggagcctgcc catctggtcc
      661 atctgcctgg gctgtttcag ccaccagtgg ctggtgtcca ccatggtggt gtacatccct
      721 acatatatct ctagcgtgta ccacgtgaac atcagggaca atggactgct gagcgccctg
      781 cccttcatcg tggcatgggt catcggaatg gtgggaggct atctggccga tttcctgctg
      841 accaagaagt ttcggctgat caccgtgaga aagatcgcca caatcctggg ctccctgccc
      901 tcctctgccc tgatcgtgtc tctgccttac ctgaacagcg gctatatcac cgcaacagcc
      961 ctgctgaccc tgtcctgcgg actgagcacc ctgtgccagt ccggcatcta catcaatgtg
     1021 ctggacatcg ccccccggta tagctccttc ctgatgggcg cctctagagg cttttctagc
     1081 atcgcccctg tgatcgtgcc aaccgtgtct ggcttcctgc tgagccagga ccctgagttt
     1141 ggctggagaa acgtgttctt tctgctgttc gccgtgaatc tgctgggcct gctgttctat
     1201 ctgatctttg gcgaggccga tgtgcaggag tgggccaagg agaggaagct gacacgcctg
//
