LOCUS       sequence-253234-        1542 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_MFSD4A
FEATURES             Location/Qualifiers
     source          1..1542
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1126..1135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgggatgcg acggacgggt gagcggactg ctgcggagaa acctgcagcc aaccctgaca
       61 tactggagcg tgttcttttc cttcggcctg tgcatcgcct ttctgggccc taccctgctg
      121 gatctgagat gtcagacaca cagctccctg ccacagatct cttgggtgtt ctttagccag
      181 cagctgtgcc tgctgctggg atccgccctg ggaggcgtgt tcaagcggac cctggcacag
      241 agcctgtggg cactgtttac atctagcctg gccatctccc tggtgttcgc cgtgatcccc
      301 ttttgcagag acgtgaaggt gctggcatct gtgatggcac tggcaggact ggcaatggga
      361 tgtatcgaca ccgtggccaa catgcagctg gtgaggatgt atcagaagga tagcgccgtg
      421 ttcctgcagg tgctgcactt ctttgtgggc tttggcgccc tgctgagccc tctgatcgcc
      481 gatccattcc tgtccgaggc caattgcctg cccgccaaca gcaccgccaa taccacatcc
      541 aggggccacc tgtttcacgt gtcccgcgtg ctgggacagc accacgtgga cgcaaagcct
      601 tggtctaacc agaccttccc aggactgaca cctaaggatg gagcaggcac aagggtgagc
      661 tacgcctttt ggatcatggc cctgatcaat ctgcctgtgc caatggccgt gctgatgctg
      721 ctgagcaagg agcgcctgct gacctgctgt ccacagaggc gccccctgct gctgtccgcc
      781 gacgagctgg ccctggagac acagccccct gagaaggagg atgcctcctc tctgccaccc
      841 aagttccagt ctcacctggg ccacgaggac ctgtttagct gctgtcagcg gaagaacctg
      901 agaggcgccc cttatagctt ctttgccatc cacatcaccg gcgccctggt gctgttcatg
      961 accgatggcc tgacaggcgc ctacagcgcc ttcgtgtact cttatgccgt ggagaagcca
     1021 ctgtccgtgg gacacaaggt ggcaggctat ctgccctctc tgttctgggg ctttatcacc
     1081 ctgggccggc tgctgtccat ccccatcagc tccagaatga agcctgccac catggtgttc
     1141 atcaacgtgg tgggcgtggt ggtgacattc ctggtgctgc tgatcttttc ttacaatgtg
     1201 gtgttcctgt ttgtgggaac cgcaagcctg ggactgttcc tgtctagcac ctttccctcc
     1261 atgctggcct acacagagga ctctctgcag tataagggct gcgcaaccac agtgctggtg
     1321 acaggagcag gagtgggaga gatggtgctg cagatgctgg tgggctccat cttccaggcc
     1381 cagggctcct actctttcct ggtgtgcggc gtgatctttg gctgtctggc cttcaccttt
     1441 tatatcctgc tgctgttctt tcacagaatg caccccggcc tgccttctgt gccaacacag
     1501 gataggtcta tcggcatgga gaatagcgag tgttatcagc gc
//
