LOCUS       sequence-253240-        3063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC12A3
FEATURES             Location/Qualifiers
     source          1..3063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcagagc tgccaaccac agagacccct ggcgacgcca cactgtgctc tggccggttt
       61 accatcagca cactgctgag ctccgacgag ccatctccac ctgcagccta cgattctagc
      121 caccctagcc acctgaccca ctcctctaca ttctgtatga gaacctttgg ctataacaca
      181 atcgacgtgg tgccaaccta cgagcactat gccaattcca cacagccagg agagccaagg
      241 aaggtgagac ccaccctggc cgatctgcac tcttttctga agcaggaggg caggcacctg
      301 cacgcactgg ccttcgactc taggcctagc cacgagatga cagatggact ggtggaggga
      361 gaggcaggca ccagctccga gaagaaccct gaggagccag tgcggttcgg ctgggtgaag
      421 ggcgtgatga tcagatgcat gctgaatatc tggggcgtga tcctgtacct gaggctgcca
      481 tggatcaccg cacaggcagg catcgtgctg acatggatca tcatcctgct gtccgtgacc
      541 gtgacatcta tcaccggcct gtctatcagc gccatcagca caaacggcaa ggtgaagtcc
      601 ggcggcacct actttctgat ctcccgctct ctgggaccag agctgggagg ctccatcggc
      661 ctgatcttcg cctttgccaa cgcagtggga gtggcaatgc acaccgtggg attcgccgag
      721 acagtgaggg atctgctgca ggagtatggc gcccccatcg tggaccctat caatgatatc
      781 cgcatcatcg ccgtggtgtc tgtgacagtg ctgctggcca tcagcctggc cggcatggag
      841 tgggagtcca aggcccaggt gctgttcttc ctggtcatca tggtgagctt cgccaactac
      901 ctggtgggca ccctgatccc acccagcgag gacaaggcct ccaagggctt cttttcttat
      961 agggccgata tctttgtgca gaatctggtg ccagactgga ggggaccaga tggcacattc
     1021 tttggcatgt tcagcatctt ctttccatcc gccaccggaa tcctggcagg agcaaacatc
     1081 tccggcgacc tgaaggatcc tgccatcgcc atcccaaagg gcacactgat ggccatcttt
     1141 tggaccacaa tctcttacct ggccatcagc gccaccatcg gatcctgcgt ggtgcgggac
     1201 gcaagcggcg tgctgaatga tactgtgaca ccaggatggg gagcatgcga gggactggca
     1261 tgtagctacg gctggaactt caccgagtgc acacagcagc actcctgtca ctatggcctg
     1321 atcaattact atcagaccat gtccatggtg tctggattcg caccactgat caccgcagga
     1381 atctttggcg ccacactgtc tagcgccctg gcatgtctgg tgagcgccgc aaaggtgttt
     1441 cagtgcctgt gcgaggacca gctgtaccct ctgatcggct tctttggcaa gggctatggc
     1501 aagaacaagg agccagtgcg gggctacctg ctggcctatg ccatcgccgt ggccttcatc
     1561 atcatcgccg agctgaacac aatcgccccc atcatctcca atttctttct gtgctcttac
     1621 gccctgatca acttctcttg ttttcacgcc tctatcacca atagcccagg ctggagaccc
     1681 agcttccagt actataataa gtgggccgcc ctgtttggcg ccatcatctc cgtggtcatc
     1741 atgttcctgc tgacctggtg ggccgccctg atcgcaatcg gagtggtgct gtttctgctg
     1801 ctgtacgtga tctataagaa gcctgaggtg aactggggct cctctgtgca ggccggcagc
     1861 tacaatctgg ccctgagcta ttccgtgggc ctgaacgagg tggaggacca catcaagaat
     1921 tacagaccac agtgcctggt gctgacagga cctccaaact tccggcccgc cctggtggat
     1981 ttcgtgggca cctttacaag aaatctgtcc ctgatgatct gtggccacgt gctgatcggc
     2041 ccccacaagc agcggatgcc tgagctgcag ctgatcgcca acggccacac caagtggctg
     2101 aataagagaa agatcaaggc cttttatagc gacgtgatcg ccgaggatct gcggagagga
     2161 gtgcagatcc tgatgcaggc agcaggactg ggaaggatga agcccaacat cctggtggtg
     2221 ggcttcaaga agaattggca gtccgcccac cctgcaaccg tggaggacta catcggcatc
     2281 ctgcacgacg ccttcgactt caactatggc gtgtgcgtga tgaggatgcg cgagggcctg
     2341 aacgtgtcta agatgatgca ggcccacatc aatcccgtgt tcgacccagc cgaggatggc
     2401 aaggaggcaa gcgccagggt ggacccaaag gccctggtga aggaggagca ggccaccaca
     2461 atctttcagt ccgagcaggg caagaagaca atcgatatct actggctgtt cgacgatggc
     2521 ggcctgaccc tgctgatccc ttatctgctg ggcaggaaga ggcgctggag caagtgcaag
     2581 atccgcgtgt ttgtgggcgg ccagatcaac cggatggatc aggagagaaa ggccatcatc
     2641 agcctgctgt ccaagttccg gctgggcttt cacgaggtgc acatcctgcc agacatcaac
     2701 cagaatccca gggccgagca caccaagcgg ttcgaggata tgatcgcccc tttcaggctg
     2761 aacgacggct ttaaggatga ggccacagtg aatgagatgc ggagagactg tccatggaag
     2821 atctccgatg aggagatcac caagaaccgc gtgaagtctc tgaggcaggt gcgcctgaat
     2881 gagatcgtgc tggactactc tcgggatgcc gccctgatcg tgatcacact gcctatcggc
     2941 agaaagggca agtgcccaag ctccctgtat atggcctggc tggagaccct gagccaggac
     3001 ctgaggccac ctgtgatcct gatcagaggc aaccaggaga atgtgctgac cttctactgt
     3061 cag
//
