LOCUS       sequence-253250-        1887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC7A1
FEATURES             Location/Qualifiers
     source          1..1887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgggctgca aggtgctgct gaacatcggc cagcagatgc tgcggagaaa ggtggtggac
       61 tgcagcaggg aggagaccag gctgtccagg tgtctgaata catttgatct ggtggccctg
      121 ggagtgggaa gcaccctggg agccggcgtg tatgtgctgg caggagcagt ggcaagggag
      181 aacgcaggcc ctgccatcgt gatctctttt ctgatcgccg ccctggcaag cgtgctggca
      241 ggactgtgct acggagagtt cggagcaagg gtgccaaaga caggatccgc ctacctgtat
      301 tcttacgtga ccgtgggcga gctgtgggcc ttcatcacag gctggaatct gatcctgtcc
      361 tatatcatcg gcaccagctc cgtggcaagg gcatggtctg ccacatttga cgagctgatc
      421 ggccggccta tcggcgagtt ctctagaacc cacatgacac tgaacgcccc tggcgtgctg
      481 gccgagaatc cagatatctt tgccgtgatc atcatcctga tcctgaccgg cctgctgaca
      541 ctgggcgtga aggagtccgc catggtgaac aagatcttca cctgcatcaa tgtgctggtg
      601 ctgggcttta tcatggtgtc tggcttcgtg aagggcagcg tgaagaactg gcagctgacc
      661 gaggaggact ttggcaatac aagcggccgg ctgtgcctga acaatgatac caaggagggc
      721 aagccaggag tgggaggctt catgcctttc ggcttttccg gcgtgctgtc tggagcagca
      781 acatgcttct acgcctttgt gggcttcgac tgtatcgcca ccacaggcga ggaggtgaag
      841 aacccccaga aggccatccc tgtgggcatc gtggcctctc tgctgatctg ctttatcgcc
      901 tatttcggcg tgagcgccgc cctgaccctg atgatgccat acttttgtct ggacaacaat
      961 agccccctgc ctgatgcctt caagcacgtg ggatgggagg gagcaaagta tgcagtggca
     1021 gtgggaagcc tgtgcgcact gtctgccagc ctgctgggat ccatgttccc aatgcccaga
     1081 gtgatctacg ccatggccga ggacggcctg ctgttcaagt ttctggccaa cgtgaatgat
     1141 cgcaccaaga cacccatcat cgccaccctg gcatccggag cagtggcagc cgtgatggcc
     1201 ttcctgtttg acctgaagga tctggtggac ctgatgtcca tcggcacact gctggcctat
     1261 tctctggtgg cagcatgcgt gctggtgctg aggtatcagc ctgagcagcc aaatctggtg
     1321 taccagatgg cctccacctc tgatgagctg gacccagccg atcagaacga gctggccagc
     1381 acaaatgact cccagctggg ctttctgccc gaggccgaga tgttctccct gaagaccatc
     1441 ctgtctccaa agaacatgga gccctctaag atcagcggcc tgatcgtgaa tatcagcaca
     1501 tccctgatcg ccgtgctgat catcaccttt tgcatcgtga cagtgctggg aagggaggcc
     1561 ctgaccaagg gcgccctgtg ggccgtgttc ctgctggcag gaagcgccct gctgtgcgcc
     1621 gtggtgaccg gcgtgatctg gagacagccc gagagcaaga caaagctgtc ctttaaggtg
     1681 cccttcctgc ctgtgctgcc aatcctgtcc atctttgtga acgtgtacct gatgatgcag
     1741 ctggatcagg gaacctgggt gcggttcgcc gtgtggatgc tgattggctt tatcatctat
     1801 ttcggctacg gcctgtggca ctctgaggag gcaagcctgg acgcagatca ggcaagaaca
     1861 cctgacggca atctggatca gtgcaag
//
