LOCUS       sequence-253264-        3255 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC12A4
FEATURES             Location/Qualifiers
     source          1..3255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgccacact tcaccgtggt gccagtggac ggacctagga gaggcgacta cgataacctg
       61 gagggcctgt cttgggtgga ctatggcgag agagccgagc tggacgatag cgatggccac
      121 ggcaatcaca gggagagctc cccattcctg tccccactgg aggcatctag gggaatcgac
      181 tactatgatc ggaacctggc cctgtttgag gaggagctgg atatccgccc taaggtgtct
      241 agcctgctgg gcaagctggt gtcctacacc aatctgacac agggagcaaa ggagcacgag
      301 gaggcagagt ccggagaggg aaccaggcgc cgggcagcag aggccccttc tatgggcaca
      361 ctgatgggcg tgtatctgcc atgcctgcag aacatcttcg gcgtgatcct gtttctgagg
      421 ctgacctgga tggtgggaac agcaggcgtg ctgcaggccc tgctgatcgt gctgatctgc
      481 tgttgctgta ccctgctgac agccatctct atgagcgcca tcgcaaccaa tggagtggtg
      541 ccagcaggag gaagctactt catgatctcc cggtctctgg gaccagagtt tggaggagca
      601 gtgggactgt gcttctacct gggcaccaca tttgccgccg ccatgtatat cctgggcgcc
      661 atcgagatcc tgctgaccta catcgcacca cctgcagcca tcttctatcc ctccggcgcc
      721 cacgacacct ctaatgccac actgaacaat atgagggtgt acggcacaat cttcctgacc
      781 tttatgacac tggtggtgtt tgtgggcgtg aagtatgtga acaagttcgc ctccctgttt
      841 ctggcctgcg tgatcatctc tatcctgagc atctacgccg gcggcatcaa gagcatcttc
      901 gacccacccg tgtttcccgt gtgcatgctg ggcaatcgca cgctttctcg cgaccaattt
      961 gatatttgtg ccaagacagc cgtggtggat aacgagaccg tggccacaca gctgtggagc
     1021 ttcttttgcc actcccccaa tctgaccaca gacagctgtg atccttactt catgctgaac
     1081 aatgtgaccg agatccctgg aatcccagga gcagccgccg gcgtgctgca ggagaacctg
     1141 tggagcgcct acctggagaa gggcgacatc gtggagaagc acggactgcc atccgccgat
     1201 gcaccaagcc tgaaggagtc cctgccactg tatgtggtgg ccgacatcgc cacctccttc
     1261 acagtgctgg tgggcatctt ctttccttct gtgaccggca tcatggcagg atccaatcgc
     1321 tctggcgacc tgcgggatgc ccagaagagc atccccgtgg gcacaatcct ggccatcatc
     1381 accacatccc tggtgtactt ctcctctgtg gtgctgtttg gagcatgcat cgagggagtg
     1441 gtgctgaggg acaagtatgg cgatggcgtg agcaggaacc tggtggtggg caccctggca
     1501 tggccatccc catgggtcat cgtgatcggc agcttctttt ccacctgtgg agcaggactg
     1561 cagagcctga caggagcacc aaggctgctg caggcaatcg caaaggacaa catcatccct
     1621 ttcctgcggg tgtttggaca cggcaaggtc aatggcgagc caacctgggc actgctgctg
     1681 acagccctga tcgcagagct gggcatcctg atcgccagcc tggatatggt ggcccccatc
     1741 ctgtccatgt tctttctgat gtgctacctg ttcgtgaacc tggcctgtgc cgtgcagacc
     1801 ctgctgagaa cacctaattg gcgcccacgg ttcaagtact atcactgggc cctgtctttt
     1861 ctgggcatga gcctgtgcct ggccctgatg tttgtgagct cctggtacta tgccctggtg
     1921 gccatgctga tcgccggcat gatctacaag tatatcgagt accagggagc agagaaggag
     1981 tggggcgacg gaatcagggg actgagcctg tccgccgcca gatatgcact gctgaggctg
     2041 gaggagggac ctccacacac caagaactgg agacctcagc tgctggtgct gctgaagctg
     2101 gacgaggatc tgcacgtgaa gtacccaagg ctgctgacct tcgcctctca gctgaaggcc
     2161 ggcaagggcc tgacaatcgt gggcagcgtg atccagggct cttttctgga gagctatgga
     2221 gaggcacagg cagcagagca gaccatcaag aatatgatgg agatcgagaa ggtgaagggc
     2281 ttctgccagg tggtggtggc ctctaaggtg agagagggcc tggcccacct gatccagtct
     2341 tgtggcctgg gcggcatgag gcacaacagc gtggtgctgg gatggccata cggatggagg
     2401 cagtccgagg acccaagggc ctggaagacc ttcatcgata cagtgagatg caccacagca
     2461 gcacacctgg ccctgctggt gcctaagaac atcgcctttt acccaagcaa tcacgagagg
     2521 tatctggagg gccacatcga cgtgtggtgg atcgtgcacg atggcggcat gctgatgctg
     2581 ctgcccttcc tgctgagaca gcacaaagtg tggaggaagt gtagaatgag gatctttacc
     2641 gtggcccaga tggacgataa ttccatccag atgaagaagg acctggccgt gttcctgtac
     2701 cacctgagac tggaggccga ggtggaggtg gtggagatgc acaactctga tatcagcgcc
     2761 tacacctatg agagaacact gatgatggag cagaggagcc agatgctgag acagatgagg
     2821 ctgaccaaga cagagaggga gagggaggca cagctggtga aggacaggca ctccgccctg
     2881 aggctggagt ccctgtactc tgacgaggag gatgagtctg ccgtgggcgc cgacaagatc
     2941 cagatgacct ggacacgcga taagtatatg accgagacat gggacccctc tcacgcccct
     3001 gataatttcc gggagctggt gcacatcaag cctgatcaga gcaacgtgag aaggatgcac
     3061 accgccgtga agctgaatga agtgatcgtg acaagatccc acgacgccag gctggtgctg
     3121 ctgaacatgc caggaccacc taggaactct gagggcgatg agaattacat ggagtttctg
     3181 gaggtgctga ccgagggact ggagcgggtg ctgctggtga gaggaggagg cagagaagtg
     3241 atcacaatct atagc
//
