LOCUS       sequence-25327-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Hageman J, Kampinga HH
  TITLE     Computational analysis of the human HSPH/HSPA/DNAJ family and 
            cloning of a human HSPH/HSPA/DNAJ expression library.
  JOURNAL   Cell Stress Chaperones. 2009 Jan;14(1):1-21. doi: 
            10.1007/s12192-008-0060-2. Epub 2008 Aug 7.
  PUBMED    18686016
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12192-008-0060-2"; journalName: "Cell Stress Chaperones"; 
            date: "2009-01"; volume: "14"; issue: "1"; pages: "1-21"
COMMENT     Sequence Label: pcDNA5/FRT/TO HIS DNAJB2a
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             84..101
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             248..256
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccggact ctagcgttta
       61 aacttaagct tctgcagacc atgcatcatc accatcacca tggatccacc atggcatcct
      121 actacgagat cctagacgtg ccgcgaagtg cgtccgctga tgacatcaag aaggcgtatc
      181 ggcgcaaggc tctccagtgg cacccagaca aaaacccaga taataaagag tttgctgaga
      241 agaaatttaa ggaggtggcc gaggcatatg aagtgctgtc tgacaagcac aagcgggaga
      301 tttacgaccg ctatggccgg gaagggctga cagggacagg aactggccca tctcgggcag
      361 aagctggcag tggtgggcct ggcttcacct tcaccttccg cagccccgag gaggtcttcc
      421 gggaattctt tgggagtgga gacccttttg cagagctctt tgatgacctg ggccccttct
      481 cagagcttca gaaccggggt tcccgacact caggcccctt ctttaccttc tcttcctcct
      541 tccctgggca ctccgatttc tcctcctcat ctttctcctt cagtcctggg gctggtgctt
      601 ttcgctctgt ttctacatct accacctttg tccaaggacg ccgcatcacc acacgcagaa
      661 tcatggagaa cgggcaggag cgggtggaag tggaggagga tgggcagctg aagtcagtca
      721 caatcaatgg tgtcccagat gacctggcac tgggcttggn gctgagccgt cgcgagcagc
      781 agccgtcagt cacttccngg tctgggggca ctcnnntcca gcagacccct gcctcatgcc
      841 cct
//
