LOCUS       sequence-253276-        2034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC25A12
FEATURES             Location/Qualifiers
     source          1..2034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             872..886
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 atggccgtga aggtgcagac cacaaagagg ggcgacccac acgagctgcg caacatcttc
       61 ctgcagtacg ccagcaccga ggtggacggc gagaggtata tgacacccga ggattttgtg
      121 cagcggtacc tgggcctgta taatgatccc aactccaatc ctaagatcgt gcagctgctg
      181 gcaggagtgg cagaccagac caaggatggc ctgatctctt accaggagtt cctggccttt
      241 gagtccgtgc tgtgcgcccc tgactctatg ttcatcgtgg ccttccagct gtttgataag
      301 tccggcaacg gcgaggtgac ctttgagaat gtgaaggaga tcttcggcca gacaatcatc
      361 caccaccaca tccccttcaa ctgggactgt gagtttatcc ggctgcactt cggccacaac
      421 agaaagaagc acctgaatta taccgagttc acacagtttc tgcaggagct gcagctggag
      481 cacgcaaggc aggccttcgc cctgaaggat aagtctaaga gcggcatgat cagcggcctg
      541 gacttctccg atatcatggt gaccatcaga tctcacatgc tgacaccctt tgtggaggag
      601 aatctggtga gcgccgcagg aggatccatc tctcaccagg tgagcttctc ctactttaac
      661 gccttcaatt ccctgctgaa caatatggag ctggtgcgga agatctattc taccctggcc
      721 ggcacaagaa aggacgtgga ggtgaccaag gaggagttcg cacagagcgc catcagatac
      781 ggacaggtga cacctctgga gatcgacatc ctgtaccagc tggccgatct gtataacgcc
      841 tccggcaggc tgaccctggc agatatcgag aggatcgcac cactggcaga gggcgccctg
      901 ccttataatc tggcagagct gcagaggcag cagtccccag gactgggcag acccatctgg
      961 ctgcagatcg cagagagcgc ctaccggttc accctgggct ccgtggcagg agcagtggga
     1021 gcaaccgccg tgtatcccat cgacctggtg aagacaagga tgcagaacca gaggggatct
     1081 ggaagcgtgg tgggagagct gatgtacaag aattcttttg attgcttcaa gaaggtgctg
     1141 cggtacgagg gcttctttgg cctgtataga ggcctgatcc cacagctgat cggagtggca
     1201 ccagagaagg ccatcaagct gaccgtgaac gactttgtga gggataagtt cacacggaga
     1261 gacggcagcg tgccactgcc agcagaggtg ctggcaggag gctgtgccgg cggctcccag
     1321 gtcatcttca ccaaccctct ggagatcgtg aagatcaggc tgcaggtggc aggagagatc
     1381 accacaggac caagggtgtc tgccctgaat gtgctgcgcg acctgggcat cttcggcctg
     1441 tacaagggcg ccaaggcctg ctttctgcgc gatatccctt ttagcgccat ctacttccca
     1501 gtgtatgccc actgtaagct gctgctggca gatgagaacg gacacgtggg aggcctgaat
     1561 ctgctggcag caggagcaat ggcaggagtg ccagccgcca gcctggtgac ccctgccgac
     1621 gtgatcaaga caaggctgca ggtggcagca agagcaggac agaccacata ctctggcgtg
     1681 atcgattgct tccggaagat cctgagagag gagggcccaa gcgccttttg gaagggaacc
     1741 gcagcaaggg tgtttcgcag ctccccacag ttcggagtga ccctggtgac atacgagctg
     1801 ctgcagcggt ggttttatat cgacttcgga ggcctgaagc cagcaggatc tgagcctaca
     1861 ccaaagagca ggatcgcaga cctgccacct gcaaaccctg atcacatcgg aggatacagg
     1921 ctggcaaccg ccacatttgc cggcatcgag aataagttcg gcctgtatct gcctaagttt
     1981 aagtccccat ctgtggccgt ggtgcagccc aaggccgccg tggccgccac ccag
//
