LOCUS       sequence-253280-        1440 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_MFSD12
FEATURES             Location/Qualifiers
     source          1..1440
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1334..1343
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgggacctg gaccacctgc agcaggagca gccccaagcc cccgccctct gtccctggtg
       61 gcaaggctga gctacgcagt gggccacttt ctgaacgacc tgtgcgcctc catgtggttc
      121 acctacctgc tgctgtatct gcactctgtg agagcatata gctccagggg agcaggactg
      181 ctgctgctgc tgggacaggt ggcagacgga ctgtgcacac cactggtggg atacgaggca
      241 gatagggcag caagctgctg tgcaagatat ggaccaagga aggcatggca cctggtggga
      301 accgtgtgcg tgctgctgtc tttccccttt atcttcagcc cttgcctggg ctgtggagca
      361 gcaacaccag agtgggccgc cctgctgtac tatggccctt ttatcgtgat ctttcagttc
      421 ggctgggcct ccacccagat ctcccacctg tctctgatcc ccgagctggt gaccaacgac
      481 cacgagaagg tggagctgac agccctgaga tacgccttca ccgtggtggc aaatatcaca
      541 gtgtatggag cagcatggct gctgctgcac ctgcagggat ctagcagggt ggagccaacc
      601 caggacatca gcatctccga tcagctggga ggacaggatg tgcccgtgtt ccggaacctg
      661 agcctgctgg tggtgggagt gggagccgtg ttttccctgc tgttccacct gggcaccaga
      721 gagcggagaa ggccacacgc cgaggagcca ggagagcaca cccctctgct ggcaccagca
      781 acagcacagc cactgctgct gtggaagcac tggctgagag agcccgcctt ttaccaagtg
      841 ggcatcctgt atatgaccac aaggctgatc gtgaatctgt ctcagaccta catggccatg
      901 tacctgacat atagcctgca cctgcctaag aagttcatcg ccaccatccc actggtcatg
      961 tacctgtctg gctttctgtc ctctttcctg atgaagccta tcaacaagtg catcggcaga
     1021 aatatgacat acttcagcgg actgctggtc atcctggcct tcgccgcctg ggtggccctg
     1081 gccgagggcc tgggcgtggc cgtgtacgca gcagccgtgc tgctgggagc aggatgtgca
     1141 accatcctgg tgacaagcct ggcaatgacc gcagacctga tcggaccaca cacaaactcc
     1201 ggcgccttcg tgtacggctc tatgagcttc ctggataagg tggccaatgg cctggccgtg
     1261 atggccatcc agtccctgca cccatgcccc tctgagctgt gctgtagggc ctgcgtgagc
     1321 ttctaccact gggccatggt ggcagtgacc ggaggagtgg gagtggcagc cgccctgtgc
     1381 ctgtgctctc tgctgctgtg gcctacaagg ctgcgccggt gggacagaga tgcaaggcca
//
