LOCUS       sequence-253288-        3417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC12A5
FEATURES             Location/Qualifiers
     source          1..3417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtctcgga gattcaccgt gacaagcctg ccacctgcag gaccagcacg ctctccagac
       61 ccagagtcca ggcgccactc tgtggcagat ccaaggcacc tgccaggaga ggacgtgaag
      121 ggcgatggca acccaaagga gagctccccc ttcatcaata gcaccgacac agagaagggc
      181 aaggagtatg atggcaagaa catggccctg tttgaggagg agatggacac atccccaatg
      241 gtgtctagcc tgctgtctgg cctggccaac tacaccaatc tgccacaggg aagcagagag
      301 cacgaggagg ccgagaacaa tgagggcggc aagaagaagc ctgtgcaggc cccaaggatg
      361 ggcacattca tgggcgtgta cctgccttgc ctgcagaaca tcttcggcgt gatcctgttt
      421 ctgaggctga cctgggtggt gggaatcgca ggcatcatgg agagcttctg catggtgttt
      481 atctgctgtt cctgtaccat gctgacagcc atctctatga gcgccatcgc aacaaatgga
      541 gtggtgccag caggaggatc ctactatatg atctcccggt ctctgggacc tgagttcgga
      601 ggagcagtgg gactgtgctt ctatctgggc accacatttg ccggcgccat gtacatcctg
      661 ggcaccatcg agatcctgct ggcctatctg ttcccagcca tggccatctt taaggcagag
      721 gacgcaagcg gagaggcagc agccatgctg aacaatatga gagtgtatgg cacctgcgtg
      781 ctgacatgta tggccaccgt ggtgtttgtg ggcgtgaagt acgtgaataa gttcgccctg
      841 gtgtttctgg gctgcgtgat cctgagcatc ctggccatct acgccggcgt gatcaagtcc
      901 gccttcgacc cacccaactt tccaatctgc ctgctgggca atagaaccct gtccaggcac
      961 ggcttcgacg tgtgcgcaaa gctggcatgg gagggcaacg agacagtgac cacaagactg
     1021 tggggcctgt tctgctcctc taggtttctg aatgccacct gtgatgagta cttcaccaga
     1081 aacaatgtga ccgagatcca gggaatccca ggagcagcaa gcggactgat caaggagaac
     1141 ctgtggagct cctacctgac aaagggcgtg atcgtggaga ggagcggaat gacatccgtg
     1201 ggactggcag acggaacccc tatcgacatg gatcacccat acgtgttctc tgatatgaca
     1261 agctacttca ccctgctggt gggcatctac ttcccatccg tgaccggaat catggcagga
     1321 tctaatagaa gcggcgacct gagggatgcc cagaagagca tccctaccgg cacaatcctg
     1381 gccatcgcca ccacatccgc cgtgtatatc tctagcgtgg tgctgttcgg agcatgcatc
     1441 gagggagtgg tgctgagaga caagtttggc gaggccgtga acggcaatct ggtggtgggc
     1501 accctggcat ggcctagccc atgggtcatc gtgatcggct ccttcttttc tacatgtgga
     1561 gcaggactgc agtccctgac cggagcacct aggctgctgc aggcaatctc tagggacgga
     1621 atcgtgccat tcctgcaggt gtttggccac ggcaaggcca acggcgagcc aacatgggcc
     1681 ctgctgctga ccgcctgcat ctgtgagatc ggcatcctga tcgcctctct ggatgaggtg
     1741 gcccccatcc tgagcatgtt ctttctgatg tgctacatgt tcgtgaacct ggcctgtgcc
     1801 gtgcagacac tgctgaggac ccccaattgg aggcctcgct tccggtacta tcactggacc
     1861 ctgtcctttc tgggcatgtc tctgtgcctg gccctgatgt ttatctgtag ctggtactat
     1921 gcactggtgg caatgctgat cgcaggactg atctacaagt atatcgagta tagaggcgcc
     1981 gagaaggagt ggggcgatgg catcaggggc ctgagcctgt ccgccgcaag atacgcactg
     2041 ctgaggctgg aggagggacc tccacacaca aagaactgga ggcctcagct gctggtgctg
     2101 gtgcgggtgg accaggatca gaatgtggtg cacccacagc tgctgagcct gacctcccag
     2161 ctgaaggccg gcaagggcct gacaatcgtg ggctctgtgc tggagggcac cttcctggag
     2221 aaccacccac aggcacagag ggcagaggag agcatccgga gactgatgga ggccgagaag
     2281 gtgaagggct tttgccaggt ggtcatctcc tctaatctgc gggacggcgt gagccacctg
     2341 atccagtccg gaggactggg aggactgcag cacaacacag tgctggtggg ctggcctcgc
     2401 aattggcggc agaaggagga tcaccagacc tggagaaact tcatcgagct ggtgagggag
     2461 accacagcag gacacctggc cctgctggtg accaagaacg tgtccatgtt ccctggcaat
     2521 ccagagcggt tcagcgaggg cagcatcgac gtgtggtgga tcgtgcacga tggcggcatg
     2581 ctgatgctgc tgcccttcct gctgcggcac cacaaagtgt ggagaaagtg taagatgagg
     2641 atctttacag tggcccagat ggacgataat agcatccaga tgaagaagga cctgaccaca
     2701 tttctgtacc acctgcgcat caccgccgag gtggaggtgg tggagatgca cgagtccgat
     2761 atctctgcct acacatatga gaagaccctg gtcatggagc agagatccca gatcctgaag
     2821 cagatgcacc tgacaaagaa cgagagagag agggagatcc agtctatcac cgacgagagc
     2881 aggggctcca tcaggcgcaa gaacccagcc aatacacgcc tgcggctgaa tgtgccagag
     2941 gagaccgcag gcgacagcga ggagaagcct gaggaggagg tgcagctgat ccacgatcag
     3001 tctgccccaa gctgccctag ctcctctcca tcccctggag aggagccaga gggagaggga
     3061 gagaccgacc ctgagaaggt gcacctgacc tggacaaagg ataagtccgt ggccgagaag
     3121 aacaagggcc catctcccgt gagctccgag ggcatcaagg acttcttttc tatgaagcct
     3181 gagtgggaga acctgaatca gagcaacgtg cggagaatgc acaccgccgt gcgcctgaat
     3241 gaagtgatcg tgaagaagtc ccgggatgcc aagctggtgc tgctgaacat gccaggcccc
     3301 cctagaaaca ggaatggcga cgagaattat atggagttcc tggaggtgct gacagagcac
     3361 ctggatagag tgatgctggt gcgcggagga ggaagagaag tgatcaccat ctactct
//
