LOCUS       sequence-253292-        1545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC16A10
FEATURES             Location/Qualifiers
     source          1..1545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             981..989
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atggtgctgt cccaggagga gccagatagc gccaggggca cctccgaggc ccagcctctg
       61 ggccctgccc caacaggagc agcaccacct ccaggaccag gaccttccga ctctcctgag
      121 gcagccgtgg agaaggtgga ggtggagctg gcaggaccag caaccgcaga gccccacgag
      181 ccccctgagc cacccgaggg cggctggggc tggctggtca tgctggccgc catgtggtgc
      241 aacggctccg tgttcggcat ccagaatgcc tgtggcgtgc tgtttgtgag catgctggag
      301 accttcggct ccaaggacga tgacaagatg gtgtttaaga cagcctgggt gggcagcctg
      361 tccatgggca tgatcttctt ttgctgtccc atcgtgagcg tgtttaccga tctgttcggc
      421 tgcaggaaga cagcagtggt gggagcagca gtgggctttg tgggcctgat gagctcctct
      481 ttcgtgagct ccatcgagcc actgtacctg acatatggca tcatctttgc ctgcggctgt
      541 tccttcgcct accagccctc tctggtcatc ctgggccact acttcaagaa gcggctggga
      601 ctggtgaacg gaatcgtgac cgcaggctct agcgtgttca caatcctgct gccactgctg
      661 ctgagggtgc tgatcgattc cgtgggcctg ttttacaccc tgcgggtgct gtgcatcttt
      721 atgttcgtgc tgtttctggc cggcttcaca tatcggcccc tggccacctc tacaaaggac
      781 aaggagtccg gcggctctgg ctcctctctg ttttctagaa agaagttcag ccctccaaag
      841 aagatcttta atttcgccat cttcaaggtg accgcatacg ccgtgtgggc agtgggaatc
      901 cctctggccc tgtttggcta cttcgtgcca tatgtgcacc tgatgaagca cgtgaacgag
      961 cggttccagg atgagaagaa taaggaggtg gtgctgatgt gcatcggagt gacatctgga
     1021 gtgggccggc tgctgtttgg cagaatcgcc gactacgtgc ctggcgtgaa gaaggtgtat
     1081 ctgcaggtgc tgagcttctt tttcatcggc ctgatgtcta tgatgatccc actgtgcagc
     1141 atcttcggcg ccctgatcgc cgtgtgcctg atcatgggcc tgtttgatgg ctgtttcatc
     1201 tccatcatgg caccaatcgc ctttgagctg gtgggagcac aggacgtgag ccaggccatc
     1261 ggctttctgc tgggcttcat gtccatccct atgaccgtgg gaccacctat cgcaggactg
     1321 ctgagggata agctgggcag ctacgacgtg gccttttatc tggcaggagt gccaccactg
     1381 atcggaggag ccgtgctgtg cttcatccca tggatccact ccaagaagca gcgcgagatc
     1441 tctaagacca caggcaagga gaagatggag aagatgctgg agaaccagaa tagcctgctg
     1501 agctcctcta gcggcatgtt caagaaggag tctgacagca tcatc
//
