LOCUS       sequence-253312-        3450 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC12A6
FEATURES             Location/Qualifiers
     source          1..3450
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcaccccc ctgagaccac aaccaagatg gccagcgtga gattcatggt gacacccacc
       61 aagatcgacg atatccctgg cctgtccgac acatctccag atctgagctc caggtctagc
      121 tccagggtgc gcttttctag cagagagagc gtgcccgaga cctccaggtc tgagcctatg
      181 agcgagatgt ccggagcaac aacctccctg gcaacagtgg ccctggaccc accctctgat
      241 cgcaccagcc acccccagga cgtgatcgag gatctgtctc agaacagcat cacaggcgag
      301 cacagccagc tgctggacga tggccacaag aaggcccgga acgcctacct gaacaattcc
      361 aattatgagg agggcgacga gtacttcgat aagaacctgg ccctgtttga ggaggagatg
      421 gacacccgcc ccaaggtgtc ctctctgctg aatcggatgg ccaactatac aaatctgacc
      481 cagggagcaa aggagcacga ggaggcagag aacatcacag agggcaagaa gaagcccaca
      541 aagacccctc agatgggcac cttcatgggc gtgtacctgc cttgcctgca gaatatcttc
      601 ggcgtgatcc tgtttctgcg gctgacatgg gtggtgggaa ccgcaggcgt gctgcaggcc
      661 tttgccatcg tgctgatctg ctgttgctgt acaatgctga ccgccatcag catgtccgcc
      721 atcgcaacca acggagtggt gccagcagga ggcagctatt tcatgatctc cagagccctg
      781 ggacctgagt ttggaggagc agtgggcctg tgcttctatc tgggcacaac ctttgccgcc
      841 gccatgtaca tcctgggcgc catcgagatc ttcctggtgt acatcgtgcc aagggccgcc
      901 atctttcact ctgacgatgc cctgaaggag agcgccgcca tgctgaacaa tatgagagtg
      961 tatggcaccg ccttcctggt gctgatggtg ctggtggtgt ttatcggcgt gaggtacgtg
     1021 aacaagttcg cctctctgtt tctggcctgc gtgatcgtga gcatcctggc catctatgcc
     1081 ggcgccatca agagctcctt cgcccctcca cactttcccg tgtgcatgct gggcaatcgc
     1141 accctgtcta gccggcacat cgacgtgtgc agcaagacaa aggagatcaa caatatgacc
     1201 gtgccatcca agctgtgggg cttcttttgc aactcctctc agttctttaa tgccacatgt
     1261 gatgagtact tcgtgcacaa caatgtgacc tccatccagg gaatcccagg actggcatct
     1321 ggcatcatca ccgagaacct gtggagcaat tatctgccaa agggcgagat catcgagaag
     1381 ccatccgcca agagctccga cgtgctgggc tctctgaacc acgagtacgt gctggtggat
     1441 atcacaacct cctttacact gctggtgggc atcttctttc cttctgtgac cggcatcatg
     1501 gccggctcca atcgctctgg cgacctgaag gatgcccaga agagcatccc aatcggcaca
     1561 atcctggcca tcctgacaac ctctttcgtg tacctgagca acgtggtgct gtttggagca
     1621 tgcatcgagg gagtggtgct gagggacaag ttcggcgatg ccgtgaaggg caatctggtg
     1681 gtgggcaccc tgagctggcc ttccccatgg gtcatcgtga tcggctcttt ctttagcaca
     1741 tgtggagcag gactgcagag cctgaccgga gcacctagac tgctgcaggc aatcgccaag
     1801 gacaacatca tcccattcct gagggtgttt ggccactcca aggccaatgg cgagccaaca
     1861 tgggcactgc tgctgaccgc agcaatcgca gagctgggca tcctgatcgc cagcctggat
     1921 ctggtggccc ctatcctgtc catgttcttt ctgatgtgct atctgttcgt gaacctggcc
     1981 tgtgccctgc agacactgct gcgcacccca aattggcggc ccagattcag gtactatcac
     2041 tgggccctgt cctttatggg catgtctatc tgcctggccc tgatgtttat ctctagctgg
     2101 tactatgcca tcgtggccat ggtcatcgcc ggcatgatct acaagtatat cgagtaccag
     2161 ggagcagaga aggagtgggg cgacggcatc cggggcctgt ctctgagcgc cgcaagattc
     2221 gcactgctga ggctggagga gggaccacct cacaccaaga actggaggcc acagctgctg
     2281 gtgctgctga agctggacga ggatctgcac gtgaagcacc cccggctgct gacattcgcc
     2341 tctcagctga aggcaggcaa gggactgacc atcgtgggaa gcgtgatcgt gggcaacttt
     2401 ctggagaatt acggagaggc cctggcagca gagcagacaa tcaagcacct gatggaggcc
     2461 gagaaggtga agggcttctg ccagctggtg gtggccgcca agctgagaga gggcatcagc
     2521 cacctgatcc agtcctgtgg cctgggcggc atgaagcaca acaccgtggt catgggctgg
     2581 cctaatggat ggagacagag cgaggacgca agggcatgga agacattcat cggaaccgtg
     2641 agggtgacaa ccgcagcaca cctggccctg ctggtggcaa agaacatctc cttctttcca
     2701 tctaatgtgg agcagttttc cgagggcaac atcgacgtgt ggtggatcgt gcacgatggc
     2761 ggcatgctga tgctgctgcc cttcctgctg aagcagcaca aagtgtggcg caagtgttcc
     2821 atccggatct ttacagtggc ccagctggag gacaattcta tccagatgaa gaaggatctg
     2881 gccaccttcc tgtaccacct gagaatcgag gccgaggtgg aggtggtgga gatgcacgac
     2941 tccgatatct ctgcctacac atatgagcgc accctgatga tggagcagcg gtctcagatg
     3001 ctgagacaca tgaggctgag caagacagag agggacaggg aggcacagct ggtgaaggat
     3061 agaaacagca tgctgaggct gaccagcatc ggctccgacg aggatgagga gacagagacc
     3121 tatcaggaga aggtgcacat gacatggacc aaggacaagt acatggcctc ccgcggccag
     3181 aaggccaagt ctatggaggg cttccaggac ctgctgaaca tgcggcccga tcagagcaat
     3241 gtgcggagaa tgcacaccgc cgtgaagctg aacgaagtga tcgtgaataa gtcccacgag
     3301 gccaagctgg tgctgctgaa tatgcctgga ccaccaagga acccagaggg cgatgagaat
     3361 tatatggagt ttctggaggt gctgacagag ggcctggagc gcgtgctgct ggtgcgggga
     3421 ggaggaagcg aagtgatcac catctactcc
//
