LOCUS       sequence-253328-        1665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Mocking TAM, Sijben HJ, Vermeulen YW, IJzerman AP, Heitman LH
  TITLE     MPP(+)-Induced Changes in Cellular Impedance as a Measure for 
            Organic Cation Transporter (SLC22A1-3) Activity and Inhibition.
  JOURNAL   Int J Mol Sci. 2022 Jan 21;23(3):1203. doi: 10.3390/ijms23031203.
  PUBMED    35163125
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-01-21"; volume: "23(3):1203. doi"; pages: " 
            10.3390/ijms23031203"
COMMENT     Sequence Label: pDONR221_SLC22A2
FEATURES             Location/Qualifiers
     source          1..1665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcctacca cagtggacga tgtgctggag cacggcggcg agtttcactt ctttcagaag
       61 cagatgttct ttctgctggc cctgctgagc gccacattcg ccccaatcta cgtgggcatc
      121 gtgtttctgg gcttcacccc tgaccacagg tgcagatccc caggagtggc agagctgagc
      181 ctgagatgtg gatggtcccc agcagaggag ctgaactaca ccgtgccagg acctggacca
      241 gcaggagagg catctcctag acagtgccgg agatatgagg tggattggaa ccagagcaca
      301 tttgactgcg tggatccact ggcctctctg gacaccaata ggagcaggct gccactggga
      361 ccttgcaggg atggctgggt gtatgagacc cccggcagct ccatcgtgac agagttcaac
      421 ctggtgtgcg ccaatagctg gatgctggac ctgttccagt ctagcgtgaa cgtgggcttc
      481 tttatcggct ctatgagcat cggctacatc gccgatcggt ttggcagaaa gctgtgcctg
      541 ctgaccacag tgctgatcaa tgcagcagca ggcgtgctga tggcaatctc ccccacctat
      601 acatggatgc tgatcttcag gctgatccag ggcctggtgt ctaaggccgg ctggctgatc
      661 ggctacatcc tgatcaccga gtttgtgggc aggcgctatc ggagaacagt gggcatcttc
      721 taccaggtgg cctataccgt gggactgctg gtgctggcag gagtggccta cgccctgcct
      781 cactggcgct ggctgcagtt cacagtgtcc ctgccaaact tctttttcct gctgtactat
      841 tggtgtatcc cagagtcccc ccggtggctg atctctcaga acaagaatgc cgaggccatg
      901 agaatcatca agcacatcgc caagaagaac ggcaagtccc tgccagcatc tctgcagcgg
      961 ctgagactgg aggaggagac aggcaagaag ctgaatccat cttttctgga cctggtgagg
     1021 actccccaga tccgcaagca cacaatgatc ctgatgtaca actggttcac ctcctctgtg
     1081 ctgtatcagg gcctgatcat gcacatgggc ctggccggcg acaatatcta cctggacttc
     1141 ttctactccg ccctggtgga gtttccagcc gccttcatga tcatcctgac catcgacagg
     1201 atcggcaggc gctacccatg ggcagcaagc aatatggtgg caggagcagc atgcctggca
     1261 tccgtgttta tccccggcga tctgcagtgg ctgaagatca tcatcagctg cctgggcagg
     1321 atgggcatca caatggccta cgagatcgtg tgcctggtga acgccgagct gtatcctacc
     1381 ttcatccgca atctgggcgt gcacatctgc agctccatgt gcgacatcgg cggcatcatc
     1441 accccctttc tggtgtacag actgacaaac atctggctgg agctgcctct gatggtgttc
     1501 ggcgtgctgg gactggtggc aggaggactg gtgctgctgc tgccagagac caagggcaag
     1561 gccctgcctg agacaatcga ggaggccgag aacatgcaga ggcctcgcaa gaataaggag
     1621 aagatgatct atctgcaggt gcagaagctg gatatcccac tgaat
//
