LOCUS       sequence-253348-        1623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC1A5
FEATURES             Location/Qualifiers
     source          1..1623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      793..802
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtggccg acccccctag agattccaag ggactggcag cagcagagcc tacagccaac
       61 ggcggcctgg ccctggccag catcgaggac cagggagcag cagcaggagg atactgcggc
      121 tccagggatc aggtgcggag atgtctgcgc gccaatctgc tggtgctgct gaccgtggtg
      181 gccgtggtgg ccggcgtggc cctgggcctg ggcgtgtctg gagcaggagg cgccctggcc
      241 ctgggacctg agcggctgag cgccttcgtg tttccaggag agctgctgct gaggctgctg
      301 aggatgatca tcctgcctct ggtggtgtgc agcctgatcg gaggagcagc atccctggac
      361 ccaggcgccc tgggcagact gggcgcctgg gccctgctgt tctttctggt gaccacactg
      421 ctggccagcg ccctgggagt gggactggcc ctggccctgc agccaggcgc cgcctccgcc
      481 gccatcaacg cctctgtggg cgccgccggc agcgccgaga atgccccctc caaggaagtc
      541 ctggactctt ttcttgatct ggccaggaac atctttccta gcaatctggt gtccgccgcc
      601 ttccgcagct actccaccac atatgaggag cggaacatca ccggcacaag agtgaaggtg
      661 ccagtgggac aggaggtgga gggcatgaat atcctgggcc tggtggtgtt cgccatcgtg
      721 tttggagtgg ccctgaggaa gctgggacca gagggagagc tgctgatcag attctttaac
      781 tctttcaatg aggccaccat ggtgctggtg agctggatca tgtggtacgc ccctgtgggc
      841 atcatgttcc tggtggccgg caagatcgtg gagatggagg acgtgggact gctgtttgca
      901 aggctgggca agtatatcct gtgctgtctg ctgggacacg caatccacgg actgctggtg
      961 ctgcccctga tctacttcct gtttacaagg aagaacccct atcgcttcct gtggggcatc
     1021 gtgacccctc tggccacagc ctttggcacc agctcctcta gcgccaccct gcctctgatg
     1081 atgaagtgcg tggaggagaa caatggcgtg gccaagcaca tctcccggtt catcctgcca
     1141 atcggcgcca cagtgaatat ggacggagcc gccctgttcc agtgcgtggc agccgtgttt
     1201 atcgcccagc tgtcccagca gtctctggat tttgtgaaga tcatcacaat cctggtgacc
     1261 gccacagcct cctctgtggg agcagcagga atcccagcag gaggcgtgct gaccctggca
     1321 atcatcctgg aggccgtgaa cctgcccgtg gatcacatct ccctgatcct ggccgtggac
     1381 tggctggtgg ataggtcttg taccgtgctg aacgtggagg gcgacgcact gggagcagga
     1441 ctgctgcaga attacgtgga tcggacagag tctagaagca ccgagccaga gctgatccag
     1501 gtgaagagcg agctgccact ggacccactg ccagtgccta cagaggaggg caatccactg
     1561 ctgaagcact ataggggacc agcaggcgat gcaaccgtgg catctgagaa ggagagcgtg
     1621 atg
//
