LOCUS       sequence-253352-        1665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC22A20P
FEATURES             Location/Qualifiers
     source          1..1665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1555..1564
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggccttca ccgacctgct ggatgcactg ggaagcatgg gccggtttca gctgaaccac
       61 acagccctgc tgctgctgcc atgcggactg ctggcatgtc acaacttcct gcagaacttc
      121 accgcagcag tgccacctca ccactgcagg ggaccagcaa accacaccga ggccagcaca
      181 aatgactccg gagcatggct gagggcaacc atccctctgg atcagctggg agcacctgag
      241 ccatgtcgga gattcacaaa gccacagtgg gccctgctgt ccccaaacag ctccatccct
      301 ggagcagcaa ccgagggatg caaggacgga tgggtgtaca atcggagcgt gttcccaagc
      361 accatcgtga tggagtggga cctggtgtgc gaggcacgga cactgagaga tctggcccag
      421 tccgtgtaca tggcaggcgt gctggtggga gcagccgtgt tcggctctct ggcagatagg
      481 ctgggatgca agggaccact ggtgtggtct tatctgcagc tggcagcaag cggagcagca
      541 accgcctact tctctagctt ttctgcctat tgcgtgttcc ggttcctgat gggcatgaca
      601 ttcagcggca tcatcctgaa tagcgtgtcc ctggtggtgg agtggatgcc taccagggga
      661 aggacagtgg caggaatcct gctgggctac tcttttaccc tgggacagct gatcctggca
      721 ggagtggcct atctgatcag gccatggagg tgcctgcagt tcgcaatcag cgcccccttt
      781 ctgatcttct ttctgtactc ctggtggctg ccagagtcct ctaggtggct gctgctgcac
      841 ggcaagtccc agctggccgt gcagaacctg cagaaggtgg ccgccatgaa tggaaggaag
      901 gaggagggag agcgcctgac caaggaagtg atgagctcct atatccagtc cgagttcgcc
      961 agcgtgtgca catctaacag catcctggac ctgtttcgga cccccgccat cagaaaggtg
     1021 acatgctgtc tgatggtcat ctggttctcc aattctgtgg cctactatgg cctggccatg
     1081 gatctgcaga agttcggcct gagcctgtac ctggtgcagg ccctgtttgg catcatcaac
     1141 acccctgcaa tgctggtggc aaccgcaaca atgatctatg tgggcaggcg cgcaacagtg
     1201 gcatccttcc tgatcctggc cggcctgatg gtcatcgcca atatgtttgt gccagagggc
     1261 acccagatcc tgtgcacagc acaggccgcc ctgggcaagg gatgcctggc ctctagcttc
     1321 atctgcgtgt acctgttcac cggcgagctg tatcccacag agatccggca gatgggaatg
     1381 ggattcgcaa gcgtgcacgc aagactggga ggactgaccg cacctctggt gaccacactg
     1441 ggcgagtact ccaccatcct gccacccgtg tcttttggcg caacagcaat cctggcagga
     1501 ctggccgtgt gcgtgctgac cgagacaagg aacatgcccc tggtggagac catcgccgcc
     1561 atggagcgga gagtgaagga gggctcctct aagaagcacg tggaggagaa gagcgaggag
     1621 atctccctgc agcagctgag ggcaagccct ctgaaggaga caatc
//
