LOCUS       sequence-253356-        1932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC24A3
FEATURES             Location/Qualifiers
     source          1..1932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(724..741)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 atgaggccaa gcggcgatga ggacagagca cggagaaggc gccggagaag gcgccggaga
       61 gatctgctgc tgtcccagct gtgcttcctg gcctctgtgg ccctgctgct gtggagcctg
      121 agctccctga gggagcagaa ggagctggat ctgatggacc tggtgggcga ggaccggaag
      181 tggatgatgg ccagaaagct gatgcaagtg aatgataccc tgacaagcga ggacgcagga
      241 ctgaggaact ccaagaattg cacagagcca gccctgcacg agttccccaa cgatatcttt
      301 accaatgagg acaggcgcca gggagcagtg gtgctgcacg tgctgtgcgc catctacatg
      361 ttctatgccc tggccatcgt gtgcgacgat ttctttgtgc caagcctgga gaagatctgc
      421 gagaggctgc acctgtccga ggatgtggca ggagccacct tcatggcagc aggatctagc
      481 gcccccgagc tgttcacctc cgtgatcggc gtgtttatca caaagggcga cgtgggagtg
      541 ggaaccatcg tgggatctgc cgtgttcaat atcctgtgca tcatcggcgt gtgcggactg
      601 tttgcaggac aggtggtggc cctgtcctct tggtgtctgc tgcgggattc tatctactat
      661 accctgagcg tgatcgccct gatcgtgttc atctacgacg agaaggtgag ctggtgggag
      721 tccctggtgc tggtgctgat gtacctgatc tatatcgtga tcatgaagta taacgcctgc
      781 atccaccagt gttttgagcg gagaacaaag ggcgccggca acatggtgaa tggcctggcc
      841 aacaatgccg agatcgacga tagctccaac tgcgatgcca ccgtggtgct gctgaagaag
      901 gccaatttcc acaggaaggc ctccgtgatc atggtggacg agctgctgtc tgcctaccct
      961 caccagctga gcttctccga ggcaggactg cgcatcatga tcacctccca ctttccccct
     1021 aagacaaggc tgtccatggc ctctcgcatg ctgatcaacg agcggcagag actgatcaac
     1081 tctcgggcct acaccaatgg cgagagcgag gtggccatca agatcccaat caagcacacc
     1141 gtggagaacg gcacaggccc ttctagcgcc ccagatagag gcgtgaatgg cacaaggagg
     1201 gacgatgtgg tggcagaggc aggaaacgag accgagaacg agaatgagga taacgagaat
     1261 gacgaggagg aggaggagga tgaggacgat gacgagggcc catacacccc cttcgacaca
     1321 ccttctggca agctggagac cgtgaagtgg gcctttacat ggccactgag cttcgtgctg
     1381 tacttcaccg tgcccaactg taataagcct cggtgggaga agtggttcat ggtgaccttt
     1441 gcctcctcta cactgtggat cgccgccttc agctacatga tggtgtggat ggtgaccatc
     1501 atcggctata cactgggcat ccccgatgtg atcatgggca tcacctttct ggcagcaggc
     1561 acatccgtgc ctgactgcat ggcctctctg atcgtggcca gacagggcat gggcgatatg
     1621 gccgtgtcta acagcatcgg ctccaacgtg ttcgacatcc tgatcggact gggactgcca
     1681 tgggcactgc agacactggc cgtggactac ggctcctata tcaggctgaa ttctcgcggc
     1741 ctgatctaca gcgtgggact gctgctggca agcgtgttcg tgaccgtgtt tggcgtgcac
     1801 ctgaacaagt ggcagctgga taagaagctg ggctgcggct gtctgctgct gtatggcgtg
     1861 ttcctgtgct tttccatcat gaccgagttt aacgtgttca catttgtgaa tctgcctatg
     1921 tgcggcgacc ac
//
