LOCUS       sequence-25337-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Hageman J, Kampinga HH
  TITLE     Computational analysis of the human HSPH/HSPA/DNAJ family and 
            cloning of a human HSPH/HSPA/DNAJ expression library.
  JOURNAL   Cell Stress Chaperones. 2009 Jan;14(1):1-21. doi: 
            10.1007/s12192-008-0060-2. Epub 2008 Aug 7.
  PUBMED    18686016
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12192-008-0060-2"; journalName: "Cell Stress Chaperones"; 
            date: "2009-01"; volume: "14"; issue: "1"; pages: "1-21"
COMMENT     Sequence Label: pcDNA5/FRT/TO HIS DNAJB10
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             84..101
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             248..256
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccggact ctagcgttta
       61 aacttaagct tctgcagacc atgcatcatc accatcacca tggatccacc atggcatcct
      121 actacgagat tctagacgta ccgcggagtg cgttccctga tgacatcaag aaggcgtacc
      181 gaaagaaggc tctgcagtgg cacccagaca agaacccgga taataaagaa tttgctgaaa
      241 aaaaatttaa ggaggtggca gaggcctatg aagtactatc tgacaagcac aaacgggaga
      301 tctatgaccg ctatggccgg gaagggctga ccggggcagg aagtggtcct tctcgatcgg
      361 aaactggtgg tgcggggcct ggcttcacat tcaccttccg tagccccgag gaagtcttcc
      421 gggagttctt cgggagcgga gacccttttt cagagctctt tgatgacttg ggtgtcttct
      481 cggagcttca gaaccagggt ccccgactca cgggcccttt cttcactttc tcttcttcct
      541 ttcctgccaa ctccgatttc tcctcctcat ctttctcctt cagcccgggg gctggtgctt
      601 tccgctccgt ttctacgtcc accacctttg tccaaggccg ccgcatcacc acacgcagaa
      661 tcatggagaa cgggcaggag cgggtagaag tggaagagga tggacaactg aagtcagtgt
      721 caatcaatgg tgtcccagat gacctggcac tangcttgga gctgagccgt cgtgagcagc
      781 aaccttcagt tgcccctggg ctgggggtca tgcaggtccc ggccgacctc tctctctcgt
      841 ccccctgacc atgatct
//
