LOCUS       sequence-253376-        2058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC22A23
FEATURES             Location/Qualifiers
     source          1..2058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      953..962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggccatcg atcggagaag ggaggcagca ggaggaggac caggccgcca gcctgcacca
       61 gcagaggaga acggatctct gccacctggc gacgccgccg ccagcgcccc actgggagga
      121 agggcaggcc caggaggagg agccgagatc cagccactgc caccactgca ccctggagga
      181 ggcccccacc cttcttgctg tagcgccgca gcagcacctt ccctgctgct gctggactac
      241 gatggctctg tgctgccatt cctgggagga ctgggaggag gatatcagaa gacactggtg
      301 ctgctgacct ggatccccgc cctgttcatc ggctttagcc agttctctga cagcttcctg
      361 ctggatcagc ctaacttttg gtgcagggga gcaggcaagg gaaccgagct ggcaggagtg
      421 accacaaccg gaaggggagg cgatatgggc aattggacaa gcctgccaac aacccccttc
      481 gccaccgcac catgggaggc agcaggaaac cgctccaata gctccggagc agacggaggc
      541 gatacccctc cactgccaag cccacctgac aagggcgata acgcctccaa ttgcgactgt
      601 agagcctggg attacggcat cagggccggc ctggtgcaga acgtggtgtc taagtgggac
      661 ctggtgtgcg ataatgcctg gaaggtgcac atcgccaagt tcagcctgct ggtgggcctg
      721 atctttggct atctgatcac aggctgtatc gcagactggg tgggccgccg gcctgtgctg
      781 ctgttttcta tcatcttcat cctgatcttt ggcctgaccg tggccctgag cgtgaacgtg
      841 acaatgttct ccaccctgag attctttgag ggcttttgcc tggccggcat catcctgaca
      901 ctgtacgcac tgaggatcga gctgtgccca ccaggcaagc ggttcatgat caccatggtg
      961 gcatccttcg tggcaatggc aggacagttt ctgatgccag gactggccgc cctgtgcagg
     1021 gattggcagg tgctgcaggc cctgatcatc tgtccattcc tgctgatgct gctgtattgg
     1081 agcatctttc ccgagtccct gcggtggctg atggccacac agcagttcga gagcgccaag
     1141 agactgatcc tgcactttac ccagaagaac aggatgaatc ccgagggcga catcaagggc
     1201 gtgatccctg agctggagaa ggagctgtcc agaaggccaa agaaggtgtg catcgtgaag
     1261 gtggtgggca caagaaacct gtggaagaat atcgtggtgc tgtgcgtgaa ctccctgacc
     1321 ggctacggca tccaccactg tttcgccagg tctatgatgg gccacgaggt gaaggtgccc
     1381 ctgctggaga acttctacgc cgattactat acaaccgcct ctatcgccct ggtgagctgc
     1441 ctggccatgt gcgtggtggt gagattcctg ggccggagag gaggactgct gctgtttatg
     1501 atcctgaccg ccctggcaag cctgctgcag ctgggactgc tgaatctgat cggcaagtac
     1561 tcccagcacc ctgactctgg catgtccgac tctgtgaagg ataagttctc catcgccttt
     1621 tctatcgtgg gaatgttcgc atcccacgca gtgggaagcc tgtccgtgtt cttttgcgcc
     1681 gagatcacac ctaccgtgat cagatgtgga ggactgggac tggtgctggc atctgccgga
     1741 tttggcatgc tgacagcccc aatcatcgag ctgcacaacc agaagggcta tttcctgcac
     1801 cacatcatct ttgcctgctg taccctgatc tgcatcatct gtatcctgct gctgcctgag
     1861 tctagagatc agaatctgcc agagaacatc agcaatggcg agcactacac aaggcagcct
     1921 ctgctgccac acaagaaggg agagcagcca ctgctgctga ccaacgccga gctgaaggac
     1981 tattccggac tgcacgatgc agcagcagca ggcgacacac tgcctgaggg cgccaccgcc
     2041 aatggcatga aggccatg
//
