LOCUS       sequence-253436-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC25A42
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      283..292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgggcaacg gcgtgaagga gggacccgtg cggctgcacg aggacgcaga ggccgtgctg
       61 agctcctctg tgagctccaa gcgcgatcac cggcaggtgc tgtctagcct gctgagcggc
      121 gccctggcag gcgccctggc aaagaccgca gtggcacctc tggacagaac aaagatcatc
      181 ttccaggtgt cctctaagcg gttcagcgcc aaggaggcct ttcgggtgct gtactatacc
      241 tacctgaacg agggcttcct gtctctgtgg agaggcaata gcgccaccat ggtgagggtg
      301 gtgccttacg ccgccatcca gttttctgcc cacgaggagt ataagcgcat cctgggcagc
      361 tactatggct tccggggaga ggccctgcca ccttggccaa gactgtttgc aggcgccctg
      421 gcaggaacca cagcagcatc cctgacctac ccactggatc tggtgagagc caggatggcc
      481 gtgacaccta aggagatgta tagcaacatc ttccacgtgt ttatcagaat ctccagggag
      541 gagggcctga agaccctgta ccacggcttt atgcctacag tgctgggcgt gatcccatac
      601 gccggcctga gcttctttac ctatgagaca ctgaagtccc tgcacaggga gtactctggc
      661 cggagacagc catatccctt cgagcgcatg atctttggag catgcgcagg actgatcgga
      721 cagagcgcct cctacccact ggacgtggtg aggcgccgga tgcagaccgc aggagtgaca
      781 ggatatccaa gggcatctat cgcacgcacc ctgaggacaa tcgtgagaga ggagggagcc
      841 gtgaggggac tgtataaggg cctgtccatg aattgggtga agggccccat cgccgtgggc
      901 atctctttca ccacatttga tctgatgcag atcctgctgc ggcacctgca gagc
//
