LOCUS       sequence-253446-        1731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC22A16
FEATURES             Location/Qualifiers
     source          1..1731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      647..656
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgggcagcc gccacttcga gggcatctac gaccacgtgg gccacttcgg ccggtttcag
       61 agagtgctgt atttcatctg cgcctttcag aacatctctt gtggcatcca ctacctggcc
      121 agcgtgttca tgggagtgac ccctcaccac gtgtgcaggc cacctggaaa cgtgagccag
      181 gtggtgtttc acaaccactc caattggtct ctggaggaca caggcgccct gctgagctcc
      241 ggacagaagg attacgtgac agttcaactg cagaatggtg agatctggga gctgagcagg
      301 tgttcccgca acaagcggga gaatacatct agcctgggct acgagtatac cggctctaag
      361 aaggagttcc cttgcgtgga cggctacatc tatgatcaga acacctggaa gagcaccgcc
      421 gtgacacagt ggaacctggt gtgcgatcgc aagtggctgg ccatgctgat ccagccactg
      481 ttcatgtttg gcgtgctgct gggctccgtg acattcggct atttttctga caggctgggc
      541 cggagagtgg tgctgtgggc cacctcctct agcatgttcc tgtttggcat cgccgccgcc
      601 ttcgccgtgg attactatac cttcatggcc gcccggttct ttctggccat ggtggccagc
      661 ggctacctgg tggtgggctt cgtgtatgtg atggagttta tcggcatgaa gtctagaacc
      721 tgggccagcg tgcacctgca ctccttcttt gcagtgggca ccctgctggt ggccctgaca
      781 ggctacctgg tgaggacctg gtggctgtat cagatgatcc tgtccaccgt gacagtgcca
      841 ttcatcctgt gctgttgggt gctgccagag acaccctttt ggctgctgtc tgagggccgc
      901 tacgaggagg cccagaagat cgtggacatc atggccaagt ggaaccgggc ctcctcttgc
      961 aagctgtctg agctgctgag cctggatctg cagggccccg tgagcaattc tccaaccgag
     1021 gtgcagaagc acaacctgag ctacctgttc tataattggt ccatcacaaa gagaaccctg
     1081 acagtgtggc tgatctggtt taccggctcc ctgggcttct atagcttttc cctgaactct
     1141 gtgaatctgg gcggcaacga gtacctgaat ctgttcctgc tgggcgtggt ggagatcccc
     1201 gcctatacct tcgtgtgcat cgccatggac aaagtgggca ggcgcaccgt gctggcatac
     1261 tccctgttct gctctgccct ggcctgtggc gtggtcatgg tcatcccaca gaagcactat
     1321 atcctgggcg tggtgacagc catggtgggc aagttcgcca tcggcgccgc ctttggcctg
     1381 atctacctgt atacagccga gctgtacccc accatcgtga gaagcctggc cgtgggctct
     1441 ggcagcatgg tgtgcaggct ggcctctatc ctggccccct ttagcgtgga cctgagctcc
     1501 atctggatct tcatccctca gctgtttgtg ggaaccatgg cactgctgtc cggcgtgctg
     1561 acactgaagc tgcccgagac cctgggcaag agactggcca ccacatggga ggaggccgcc
     1621 aagctggaga gcgagaatga gtccaagtct agcaagctgc tgctgaccac aaacaatagc
     1681 ggcctggaga agaccgaggc catcacacct agggattccg gcctgggcga g
//
