LOCUS       sequence-253448-        1668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Mocking TAM, Sijben HJ, Vermeulen YW, IJzerman AP, Heitman LH
  TITLE     MPP(+)-Induced Changes in Cellular Impedance as a Measure for 
            Organic Cation Transporter (SLC22A1-3) Activity and Inhibition.
  JOURNAL   Int J Mol Sci. 2022 Jan 21;23(3):1203. doi: 10.3390/ijms23031203.
  PUBMED    35163125
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-01-21"; volume: "23(3):1203. doi"; pages: " 
            10.3390/ijms23031203"
COMMENT     Sequence Label: pDONR221_SLC22A3
FEATURES             Location/Qualifiers
     source          1..1668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcctagct ttgacgaggc cctgcagagg gtgggagagt ttggcagatt ccagcggaga
       61 gtgttcctgc tgctgtgcct gaccggcgtg acattcgcct ttctgttcgt gggagtggtg
      121 ttcctgggaa cccagccaga tcactactgg tgcaggggac catccgccgc cgccctggca
      181 gagagatgtg gctggtctcc cgaggaggag tggaacagga cagcaccagc atccagggga
      241 ccagagccac ctgagaggag gggcaggtgc cagcggtacc tgctggaggc agcaaacgac
      301 tctgccagcg ccaccagcgc cctgtcctgc gcagatcctc tggcagcctt tccaaatagg
      361 tctgccccac tggtgccatg taggggagga tggagatatg cacaggcaca ctctaccatc
      421 gtgagcgagt tcgacctggt gtgcgtgaac gcctggatgc tggatctgac acaggccatc
      481 ctgaatctgg gctttctgac cggagccttc acactgggat acgcagcaga ccggtatggc
      541 agaatcgtga tctacctgct gtcttgcctg ggagtgggag tgaccggagt ggtggtggcc
      601 tttgccccta actttccagt gttcgtgatc tttcggttcc tgcagggcgt gttcggcaag
      661 ggcacctgga tgacatgtta cgtgatcgtg accgagatcg tgggctccaa gcagcggaga
      721 atcgtgggca tcgtgatcca gatgttcttt acactgggca tcatcatcct gcccggcatc
      781 gcctacttca tccctaattg gcagggcatc cagctggcca tcaccctgcc cagctttctg
      841 ttcctgctgt actattgggt ggtgccagag tcccctaggt ggctgatcac aagaaagaag
      901 ggcgacaagg ccctgcagat cctgaggcgc atcgccaagt gcaacggcaa gtacctgagc
      961 tccaattata gcgagatcac cgtgacagac gaggaggtgt ctaaccccag cttcctggat
     1021 ctggtgagga cccctcagat gcgcaagtgt acactgatcc tgatgtttgc ctggttcacc
     1081 tctgccgtgg tgtaccaggg cctggtcatg cggctgggca tcatcggcgg caacctgtat
     1141 atcgatttct ttatcagcgg agtggtggag ctgccaggcg ccctgctgat cctgctgacc
     1201 atcgagagac tgggccggag actgcctttt gccgcaagca atatcgtggc aggagtggca
     1261 tgcctggtga cagccttcct gccagaggga atcgcatggc tgaggaccac agtggccacc
     1321 ctgggcagac tgggcatcac aatggccttt gagatcgtgt acctggtgaa ctccgagctg
     1381 tatcccacca cactgaggaa ttttggcgtg tccctgtgct ctggcctgtg cgatttcggc
     1441 ggcatcatcg ccccttttct gctgttccgc ctggcagccg tgtggctgga gctgccactg
     1501 atcatcttcg gcatcctggc ctccatctgt ggcggcctgg tcatgctgct gcctgagacc
     1561 aagggcatcg ccctgccaga gacagtggac gatgtggaga agctgggcag cccacactcc
     1621 tgcaagtgtg gcaggaataa gaagaccccc gtgtcccgct ctcacctg
//
