LOCUS       sequence-253468-        1662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Mocking TAM, Sijben HJ, Vermeulen YW, IJzerman AP, Heitman LH
  TITLE     MPP(+)-Induced Changes in Cellular Impedance as a Measure for 
            Organic Cation Transporter (SLC22A1-3) Activity and Inhibition.
  JOURNAL   Int J Mol Sci. 2022 Jan 21;23(3):1203. doi: 10.3390/ijms23031203.
  PUBMED    35163125
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-01-21"; volume: "23(3):1203. doi"; pages: " 
            10.3390/ijms23031203"
COMMENT     Sequence Label: pDONR221_SLC22A1
FEATURES             Location/Qualifiers
     source          1..1662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcccaccg tggacgatat cctggagcaa gtgggcgaga gcggctggtt ccagaagcag
       61 gcctttctga tcctgtgcct gctgtccgcc gccttcgccc ctatctgcgt gggcatcgtg
      121 ttcctgggct ttaccccaga ccaccactgc cagagcccag gagtggcaga gctgtctcag
      181 agatgtggct ggagcccagc cgaggagctg aactacacag tgccaggact gggacctgca
      241 ggagaggcct ttctgggcca gtgccggaga tatgaggtgg actggaacca gtctgccctg
      301 agctgcgtgg atcctctggc ctccctggcc accaataggt ctcacctgcc actgggacca
      361 tgccaggacg gatgggtgta cgatacccca ggcagctcca tcgtgacaga gttcaacctg
      421 gtgtgcgccg actcttggaa gctggatctg tttcagagct gtctgaatgc cggcttcctg
      481 tttggcagcc tgggcgtggg ctatttcgcc gatagatttg gcaggaagct gtgcctgctg
      541 ggaaccgtgc tggtgaacgc cgtgtccggc gtgctgatgg ccttcagccc caattacatg
      601 tccatgctgc tgtttcgcct gctgcagggc ctggtgtcta agggcaattg gatggccggc
      661 tataccctga tcacagagtt cgtgggatcc ggatctagga ggacagtggc catcatgtac
      721 cagatggcct ttaccgtggg actggtggcc ctgacaggac tggcatatgc actgccacac
      781 tggaggtggc tgcagctggc cgtgtccctg cccaccttcc tgtttctgct gtactattgg
      841 tgcgtgcctg agagcccaag atggctgctg tcccagaaga ggaacacaga ggccatcaag
      901 atcatggacc acatcgccca gaagaatggc aagctgcccc ctgccgacct gaagatgctg
      961 agcctggagg aggatgtgac cgagaagctg agcccctcct tcgccgacct gtttcggacc
     1021 cctcggctga gaaagaggac attcatcctg atgtacctgt ggtttaccga ttccgtgctg
     1081 tatcagggcc tgatcctgca catgggcgcc acatccggca acctgtacct ggacttcctg
     1141 tattctgccc tggtggagat cccaggcgcc tttatcgccc tgatcacaat cgatcgcgtg
     1201 ggccggatct accccatggc aatgtctaac ctgctggcag gagcagcatg tctggtcatg
     1261 atcttcatca gccccgacct gcactggctg aatatcatca tcatgtgcgt gggccgcatg
     1321 ggcatcacca tcgccatcca gatgatctgt ctggtgaacg ccgagctgta tcctacattt
     1381 gtgcggaatc tgggcgtgat ggtgtgctct agcctgtgcg atatcggcgg catcatcacc
     1441 cctttcatcg tgtttcgcct gagggaagtg tggcaggccc tgccactgat cctgttcgcc
     1501 gtgctgggac tgctggcagc aggagtgacc ctgctgctgc ctgagacaaa gggcgtggcc
     1561 ctgccagaga ccatgaagga tgccgagaac ctgggcagaa aggccaagcc caaggagaat
     1621 acaatctacc tgaaggtgca gacctccgag ccttctggca ca
//
