LOCUS       sequence-253516-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC39A1
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(319..330)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgggaccat ggggagagcc agagctgctg gtgtggagac cagaggcagt ggcaagcgag
       61 ccacctgtgc ctgtgggact ggaggtgaag ctgggcgccc tggtgctgct gctggtgctg
      121 accctgctgt gctccctggt gcctatctgc gtgctgcgga gaccaggagc aaaccacgag
      181 ggatctgcca gcaggcagaa ggccctgagc ctggtgtcct gcttcgcagg cggcgtgttt
      241 ctggccacct gtctgctgga cctgctgcct gattacctgg cagcaatcga cgaggccctg
      301 gccgccctgc acgtgacact gcagttccca ctgcaggagt ttatcctggc catgggcttc
      361 ttcctggtgc tggtcatgga gcagatcacc ctggcctata aggagcagtc tggaccaagc
      421 ccactggagg agacccgcgc cctgctggga acagtgaatg gaggccccca gcactggcac
      481 gacggccctg gagtgccaca ggcaagcgga gcaccagcaa caccttccgc cctgagggca
      541 tgcgtgctgg tgttctccct ggccctgcac agcgtgttcg agggactggc agtgggactg
      601 cagagggata gggcaagggc aatggagctg tgcctggccc tgctgctgca caagggcatc
      661 ctggccgtgt ccctgtctct gagactgctg cagtcccacc tgagggcaca ggtggtggca
      721 ggatgcggca tcctgttctc ttgtatgacc ccactgggca tcggcctggg agccgccctg
      781 gcagagtctg ccggacccct gcaccagctg gcacagagcg tgctggaggg aatggcagcc
      841 ggcaccttcc tgtacatcac atttctggag atcctgcctc aggagctggc aagctccgag
      901 cagcggatcc tgaaagtgat cctgctgctg gcaggattcg cactgctgac aggcctgctg
      961 tttatccaga tc
//
