LOCUS       sequence-253536-        1974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC28A2
FEATURES             Location/Qualifiers
     source          1..1974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1433..1442
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggagaagg cctctggcag gcagagcatc gccctgtcca ccgtggagac cggcacagtg
       61 aacccaggcc tggagctgat ggagaaggag gtggagcccg agggcagcaa gaggaccgac
      121 gcacagggac actccctggg cgatggactg ggaccatcta cataccagcg gagaagcagg
      181 tggcctttct ctaaggcccg cagcttttgc aagacccacg cctccctgtt caagaagatc
      241 ctgctgggac tgctgtgcct ggcatacgca gcatatctgc tggcagcatg tatcctgaac
      301 ttccagcggg ccctggccct gtttgtgatc acctgcctgg tcatcttcgt gctggtgcac
      361 agctttctga agaagctgct gggcaagaag ctgaccagat gtctgaagcc cttcgagaat
      421 agcaggctgc gcctgtggac aaagtgggtg tttgcaggcg tgtccctggt gggactgatc
      481 ctgtggctgg ccctggacac cgcacagagg ccagagcagc tgatcccctt cgccggcatc
      541 tgcatgttca tcctgatcct gtttgcctgt tccaagcacc actctgccgt gagctggcgg
      601 acagtgtttt ctggcctggg cctgcagttc gtgtttggca tcctggtcat cagaaccgat
      661 ctgggctaca cagtgttcca gtggctgggc gagcaggtgc agatctttct gaactatacc
      721 gtggccggca gctccttcgt gtttggcgac acactggtga aggacgtgtt cgcctttcag
      781 gccctgccta tcatcatctt ctttggctgc gtggtgtcca tcctgtacta tctgggcctg
      841 gtgcagtggg tggtgcagaa ggtggcctgg ttcctgcaga tcaccatggg caccacagca
      901 accgagacac tggcagtggc aggcaatatc tttgtgggca tgacagaggc ccctctgctg
      961 atccggccat acctgggcga catgaccctg tctgagatcc acgccgtgat gacaggcggc
     1021 ttcgcaacca tcagcggaac agtgctggga gccttcatcg cctttggcgt ggatgcctct
     1081 agcctgatct ctgccagcgt gatggcagca ccttgtgcac tggcatcctc taagctggcc
     1141 tatccagagg tggaggagtc caagtttaag tctgaggagg gagtgaagct gccaaggggc
     1201 aaggagagaa acgtgctgga ggcagcaagc aatggagcag tggacgcaat cggactggca
     1261 accaacgtgg cagccaatct gatcgccttc ctggccgtgc tggcctttat caacgccgcc
     1321 ctgtcctggc tgggagagct ggtggacatc cagggactga ccttccaggt catctgctct
     1381 tacctgctgc ggccaatggt gtttatgatg ggcgtggagt ggacagattg tcccatggtg
     1441 gccgagatgg tgggcatcaa gttctttatc aacgagttcg tggcctacca gcagctgtcc
     1501 cagtataaga ataagaggct gtctggcatg gaggagtgga tcgagggcga gaagcagtgg
     1561 atctctgtgc gcgccgagat catcaccaca ttcagcctgt gcggctttgc caatctgagc
     1621 tccatcggca tcaccctggg cggcctgaca tccatcgtgc ctcacaggaa gtccgacctg
     1681 tctaaggtgg tggtgcgcgc cctgttcacc ggagcatgcg tgagcctgat ctccgcctgt
     1741 atggcaggaa tcctgtacgt gccaagagga gcagaggcag attgcgtgag cttccctaac
     1801 acctccttta caaatcggac ctacgagaca tatatgtgct gtagaggcct gttccagtcc
     1861 acctctctga acggcacaaa tccccctagc ttctccggcc catgggagga taaggagttt
     1921 agcgccatgg ccctgaccaa ttgctgtggc ttttataaca atacagtgtg cgcc
//
