LOCUS       sequence-25364-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Deans TL, Cantor CR, Collins JJ
  TITLE     A tunable genetic switch based on RNAi and repressor proteins for 
            regulating gene expression in mammalian cells.
  JOURNAL   Cell. 2007 Jul 27. 130(2):363-72.
  PUBMED    17662949
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jul 27. 130(2):363-72."
COMMENT     Sequence Label: TLD_009
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    81..100
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     primer_bind     complement(501..522)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtgcnccnga tctaagcttg gacaaactac ctacagagat ttaaagctct aaggtaaata
       61 taaaatttac taggttgtgg aattgtgagc gctcacaatt ccacagtcga ccctaggttg
      121 tggaatgtta aactacggat ccgtctccca ttaggcctac aatggtgaga caagtagcca
      181 acagggaagg gttgcaaata tcatttgggc acacctatga taatattgat gaagcagaca
      241 gtattcagca agtaactgag aggtgggaag ctcaaagcca aagtcctaat gtgcagtcag
      301 gtgaatttat tgaaaaattt gaggctcctg gtggtgcaaa tcaaagaact gctcctcagg
      361 gatcctaatt gtttgtgtat tttagattcc aaccaagctt gcggccgctc aggagctaag
      421 gaattgatcc tctagagtcg acctgcagca caaccatggt gagcaagggc gaggagctgt
      481 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      541 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      601 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      661 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      721 tgcccgaagg ntacgtccag gagcgcacca tcttcttcaa ggacganggc aactacaaga
      781 cccgcgccga
//
