LOCUS       sequence-25365-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Deans TL, Cantor CR, Collins JJ
  TITLE     A tunable genetic switch based on RNAi and repressor proteins for 
            regulating gene expression in mammalian cells.
  JOURNAL   Cell. 2007 Jul 27. 130(2):363-72.
  PUBMED    17662949
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jul 27. 130(2):363-72."
COMMENT     Sequence Label: TLD_026
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..680
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(133..152)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     811..830
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gagctggttt agtgaaccgt cagatcggat cctctagagt cgacctgcag gccgccacca
       61 tgtctagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
      121 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
      181 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag
      241 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta
      301 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt
      361 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat
      421 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta
      481 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac
      541 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag
      601 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac
      661 ttaaatgtga aagtgggtcc gcgtacagcg gatcccggga attcagatct tattaaagca
      721 gaanttgttt attgcagctt ataatggttn acaaataaag catagcatca caatttcaca
      781 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatg
//
