LOCUS       sequence-25368-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Deans TL, Cantor CR, Collins JJ
  TITLE     A tunable genetic switch based on RNAi and repressor proteins for 
            regulating gene expression in mammalian cells.
  JOURNAL   Cell. 2007 Jul 27. 130(2):363-72.
  PUBMED    17662949
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jul 27. 130(2):363-72."
COMMENT     Sequence Label: TLD_062
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     116..136
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     287..306
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    318..336
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(430..449)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(431..449)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(456..473)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(456..472)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(465..487)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(700..719)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agctctccca tatggtcgac ctgcaggcgg ccgcgaattc actagtgatt aggcaaaacg
       61 caccacgtga cggagcgtga ccgcgcgccg agcgcgcgcc aaggtcgggc aggaagaggg
      121 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
      181 tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
      241 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
      301 taccgtaact tgaaagtact ctatcattga tagagttata tatcttgtgg aaaggacgaa
      361 acaccgctac acaaatcagc gatttttcaa gagaaaatcg ctgatttgtg tagaaaaagg
      421 cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
      481 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      541 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      601 aatggcgaan ggacgcgccc tgtagcggcg cantaagcgc ggcgggtgtg gtggttacgc
      661 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      721 cctttctcgc cacgttcgcc ggctttcccc gtcanctcta aatcgggggc tccctttagg
      781 gttccnattt agtgctttnn ggcncctcga ccccaaaaan ttgattnagg g
//
