LOCUS       sequence-253690-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC35E4
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      493..502
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgtgcaggt gtccacctga gcaccacgac ggaaggatga ccagcgccga agtgggagca
       61 gcagcaggag gagcacaggc agccggccca cccgagtggc ctccaggctc ccctcaggcc
      121 ctgaggcagc caggaagggc cagagtggca atggccgccc tggtgtggct gctggcagga
      181 gcatccatga gctccctgaa caagtggatc ttcaccgtgc acggctttgg caggcctctg
      241 ctgctgtctg ccctgcacat gctggtggcc gccctggcat gccacagggg agcacggaga
      301 ccaatgccag gaggaacaag gtgtcgcgtg ctgctgctgt ccctgacctt cggcacatct
      361 atggcatgcg gaaatgtggg actgagggca gtgccactgg atctggccca gctggtgacc
      421 acaaccacac ccctgtttac cctggccctg tctgccctgc tgctgggcag gcgccaccac
      481 cccctgcagc tggccgccat gggccctctg tgcctgggag cagcatgtag cctggcagga
      541 gagttcagaa ccccccctac aggctgcggc tttctgctgg cagcaacatg tctgaggggc
      601 ctgaagagcg tgcagcagtc cgccctgctg caggaggaga gactggacgc cgtgaccctg
      661 ctgtacgcca catccctgcc ttctttctgc ctgctggcag gagccgccct ggtgctggag
      721 gcaggcgtgg ccccaccccc taccgccggc gattcccggc tgtgggcctg catcctgctg
      781 tcttgtctgc tgagcgtgct gtataacctg gcctctttca gcctgctggc cctgacctct
      841 gccctgacag tgcacgtgct gggcaacctg accgtggtgg gcaatctgat cctgagccgg
      901 ctgctgtttg gcagcagact gtccgccctg tcttacgtgg gcatcgccct gacactgagc
      961 ggcatgttcc tgtatcacaa ttgtgagttt gtggcctcct gggcagcaag gagaggactg
     1021 tggaggaggg accagccaag caagggcctg
//
