LOCUS       sequence-25370-e         567 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 567)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP1
FEATURES             Location/Qualifiers
     source          1..567
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..33
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             46..90
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    124..248
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(273..278,279..296,297..303)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(423..442)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggcgggggcg gggagaatct ttattttcag ggcggcnngn nnnggaaaga aaccgctgct
       61 gctaaattcg aacgccagca catggacagc ggaggtggag gtattaacta taacggtcat
      121 caaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
      181 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
      241 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
      301 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
      361 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
      421 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
      481 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
      541 ttcttgcccg cctgatgaat gctcatc
//
