LOCUS       sequence-253724-        2919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC8A1
FEATURES             Location/Qualifiers
     source          1..2919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtacaaca tgcggagact gtccctgtct cctaccttca gcatgggctt tcacctgctg
       61 gtgacagtga gcctgctgtt ctcccacgtg gatcacgtga tcgccgagac cgagatggag
      121 ggcgagggca atgagacagg cgagtgcacc ggctcctact attgtaagaa gggcgtgatc
      181 ctgccaatct gggagccaca ggatccctct tttggcgaca agatcgccag agccacagtg
      241 tatttcgtgg ccatggtgta catgtttctg ggcgtgtcta tcatcgccga caggttcatg
      301 agctccatcg aagtgatcac aagccaggag aaggagatca ccatcaagaa gccaaacggc
      361 gagaccacaa agaccacagt gagaatctgg aacgagacag tgagcaatct gaccctgatg
      421 gcactgggat ctagcgcccc agagatcctg ctgtccgtga tcgaggtgtg cggccacaac
      481 tttacagccg gcgatctggg accttctacc atcgtgggaa gcgccgcctt caatatgttt
      541 atcatcatcg ccctgtgcgt gtacgtggtg cctgacggcg agaccagaaa gatcaagcac
      601 ctgagggtgt tctttgtgac cgccgcctgg agcatcttcg cctatacatg gctgtacatc
      661 atcctgtccg tgatctctcc aggagtggtg gaagtgtggg agggactgct gacattcttt
      721 ttctttccca tctgcgtggt gttcgcatgg gtggcagaca ggcgcctgct gttttacaag
      781 tacgtgtaca agagatacag ggccggcaag cagagaggca tgatcatcga gcacgagggc
      841 gataggccct cctctaagac cgagatcgag atggacggca aggtggtgaa ctcccacgtg
      901 gagaattttc tggatggcgc cctggtgctg gaggtggatg agagggacca ggacgatgag
      961 gaggcaagga gagagatggc ccggatcctg aaggagctga agcagaagca ccctgacaag
     1021 gagatcgagc agctgatcga gctggccaac tatcaggtgc tgtcccagca gcagaagtct
     1081 cgcgccttct accggatcca ggcaacaagg ctgatgaccg gagcaggcaa tatcctgaag
     1141 aggcacgcag cagatcaggc aaggaaggcc gtgtccatgc acgaggtgaa cacagaggtg
     1201 accgagaatg accccgtgtc taagatcttc tttgagcagg gcacctatca gtgcctggag
     1261 aactgtggca cagtggccct gaccatcatc aggaggggag gcgatctgac caacacagtg
     1321 ttcgtggatt ttcgcacaga ggacggaacc gcaaatgcag gatccgatta cgagttcaca
     1381 gagggcaccg tggtgtttaa gcctggcgac acccagaagg agatccgggt gggcatcatc
     1441 gacgatgaca tcttcgagga ggatgagaac tttctggtgc acctgtctaa tgtgaaggtg
     1501 agctccgagg caagcgagga cggaatcctg gaggccaacc acgtgtccac cctggcatgc
     1561 ctgggaagcc catccaccgc cacagtgacc atcttcgatg acgatcacgc cggcatcttc
     1621 acatttgagg agcccgtgac ccacgtgtct gagagcatcg gcatcatgga ggtgaaggtg
     1681 ctgagaacct ctggcgccag gggcaatgtg atcgtgccat ataagacaat cgagggaacc
     1741 gcaaggggag gaggagagga tttcgaggac acctgtggcg agctggagtt tcagaacgac
     1801 gagatcgtga agacaatcag cgtgaaagtg atcgacgatg aggagtacga gaagaataag
     1861 accttctttc tggagatcgg agagccaagg ctggtggaga tgtccgagaa gaaggccctg
     1921 ctgctgaatg agctgggcgg cttcacaatc accggcaagt atctgttcgg ccagcccgtg
     1981 tttcgcaagg tgcacgcccg ggagcaccct atcctgtcta cagtgatcac catcgccgat
     2041 gagtacgacg ataagcagcc tctgaccagc aaggaggagg aggagcggag aatcgccgag
     2101 atgggcagac caatcctggg cgagcacaca aagctggaag tgatcatcga ggagagctat
     2161 gagttcaagt ccacagtgga caagctgatc aagaagacca acctggccct ggtggtgggc
     2221 acaaatagct ggagggagca gtttatcgag gcaatcaccg tgtccgccgg agaggacgat
     2281 gacgatgacg agtgcggcga ggagaagctg ccctcttgtt tcgattatgt gatgcacttc
     2341 ctgaccgtgt tttggaaggt gctgttcgcc tttgtgcccc ctacagagta ctggaacggc
     2401 tgggcctgct tcatcgtgag catcctgatg atcggcctgc tgaccgcctt catcggcgat
     2461 ctggcctccc actttggctg taccatcggc ctgaaggact ctgtgacagc cgtggtgttc
     2521 gtggccctgg gcacatctgt gcctgacacc tttgccagca aggtggccgc cacccaggat
     2581 cagtatgccg acgcctccat cggcaatgtg acaggctcta acgccgtgaa cgtgttcctg
     2641 ggaatcggag tggcatggag catcgcagca atctaccacg cagcaaacgg agagcagttt
     2701 aaggtgagcc caggcaccct ggccttctcc gtgacactgt ttaccatctt cgcctttatc
     2761 aatgtgggcg tgctgctgta caggcgccgg cctgagatcg gaggagagct gggaggacca
     2821 aggacagcaa agctgctgac ctcctgcctg ttcgtgctgc tgtggctgct gtatatcttc
     2881 ttttctagcc tggaggccta ctgtcacatc aagggcttt
//
